Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaA and hfq are separated by 85 nucleotides hfq and hflX are separated by 75 nucleotides hflX and hflK are separated by 85 nucleotides
b4171: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI), at 4,397,275 to 4,398,225
miaA
b4172: hfq - RNA-binding protein Hfq (NCBI), at 4,398,311 to 4,398,619
hfq
b4173: hflX - predicted GTPase (NCBI), at 4,398,695 to 4,399,975
hflX
b4174: hflK - modulator for HflB protease specific for phage lambda cII repressor (NCBI), at 4,400,061 to 4,401,320
hflK
Position (kb)
4398
4399
4400 Strain fitness (log2 ratio)
-1
0
1
2 at 4397.704 kb on - strand, within miaA at 4397.763 kb on - strand, within miaA at 4397.845 kb on - strand, within miaA at 4397.853 kb on + strand, within miaA at 4397.856 kb on + strand, within miaA at 4397.866 kb on + strand, within miaA at 4397.922 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4398.137 kb on + strand at 4398.137 kb on + strand at 4398.156 kb on + strand at 4398.233 kb on + strand at 4398.233 kb on + strand at 4398.433 kb on + strand, within hfq at 4398.562 kb on + strand, within hfq at 4398.565 kb on + strand, within hfq at 4398.593 kb on + strand at 4398.668 kb on - strand at 4398.675 kb on - strand at 4398.677 kb on + strand at 4398.677 kb on + strand at 4398.703 kb on + strand at 4398.714 kb on + strand at 4398.724 kb on - strand at 4398.728 kb on + strand at 4398.728 kb on + strand at 4398.793 kb on + strand at 4398.815 kb on - strand at 4398.815 kb on - strand at 4398.837 kb on + strand, within hflX at 4398.926 kb on - strand, within hflX at 4398.961 kb on + strand, within hflX at 4398.961 kb on + strand, within hflX at 4398.965 kb on + strand, within hflX at 4398.965 kb on + strand, within hflX at 4398.968 kb on - strand, within hflX at 4398.983 kb on - strand, within hflX at 4398.983 kb on - strand, within hflX at 4398.992 kb on - strand, within hflX at 4399.118 kb on + strand, within hflX at 4399.140 kb on + strand, within hflX at 4399.154 kb on + strand, within hflX at 4399.217 kb on - strand, within hflX at 4399.307 kb on + strand, within hflX at 4399.307 kb on + strand, within hflX at 4399.365 kb on - strand, within hflX at 4399.365 kb on - strand, within hflX at 4399.407 kb on + strand, within hflX at 4399.407 kb on + strand, within hflX at 4399.421 kb on - strand, within hflX at 4399.449 kb on - strand, within hflX at 4399.457 kb on - strand, within hflX at 4399.457 kb on - strand, within hflX at 4399.457 kb on - strand, within hflX at 4399.500 kb on - strand, within hflX at 4399.500 kb on - strand, within hflX at 4399.542 kb on + strand, within hflX at 4399.622 kb on + strand, within hflX at 4399.691 kb on + strand, within hflX at 4399.761 kb on + strand, within hflX at 4399.793 kb on - strand, within hflX at 4399.804 kb on + strand, within hflX at 4400.041 kb on + strand at 4400.041 kb on + strand at 4400.043 kb on - strand at 4400.126 kb on + strand at 4400.162 kb on + strand at 4400.162 kb on + strand at 4400.293 kb on - strand, within hflK at 4400.298 kb on + strand, within hflK at 4400.298 kb on + strand, within hflK at 4400.356 kb on + strand, within hflK at 4400.385 kb on - strand, within hflK at 4400.385 kb on - strand, within hflK at 4400.411 kb on + strand, within hflK at 4400.416 kb on + strand, within hflK at 4400.416 kb on + strand, within hflK at 4400.429 kb on - strand, within hflK at 4400.476 kb on - strand, within hflK at 4400.502 kb on - strand, within hflK at 4400.534 kb on - strand, within hflK at 4400.546 kb on - strand, within hflK at 4400.555 kb on - strand, within hflK at 4400.582 kb on + strand, within hflK at 4400.586 kb on + strand, within hflK at 4400.586 kb on + strand, within hflK at 4400.620 kb on - strand, within hflK at 4400.643 kb on + strand, within hflK at 4400.654 kb on + strand, within hflK at 4400.704 kb on - strand, within hflK at 4400.704 kb on - strand, within hflK at 4400.709 kb on - strand, within hflK at 4400.716 kb on + strand, within hflK at 4400.716 kb on + strand, within hflK at 4400.747 kb on + strand, within hflK at 4400.749 kb on + strand, within hflK at 4400.767 kb on - strand, within hflK at 4400.779 kb on - strand, within hflK at 4400.779 kb on - strand, within hflK at 4400.860 kb on + strand, within hflK at 4400.875 kb on - strand, within hflK at 4400.908 kb on - strand, within hflK at 4400.932 kb on + strand, within hflK at 4400.933 kb on + strand, within hflK at 4400.942 kb on + strand, within hflK at 4400.942 kb on + strand, within hflK at 4400.942 kb on - strand, within hflK at 4400.951 kb on - strand, within hflK at 4400.951 kb on - strand, within hflK at 4400.952 kb on + strand, within hflK at 4400.952 kb on + strand, within hflK at 4400.952 kb on + strand, within hflK at 4400.966 kb on + strand, within hflK
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 4,397,704 - miaA b4171 0.45 -0.8 4,397,763 - miaA b4171 0.51 -0.6 4,397,845 - miaA b4171 0.60 +0.2 4,397,853 + miaA b4171 0.61 +1.7 4,397,856 + miaA b4171 0.61 -0.1 4,397,866 + miaA b4171 0.62 -0.7 4,397,922 + miaA b4171 0.68 -0.4 4,397,935 + miaA b4171 0.69 -0.8 4,397,935 + miaA b4171 0.69 +0.2 4,398,137 + +0.2 4,398,137 + -1.2 4,398,156 + +0.9 4,398,233 + +0.3 4,398,233 + +0.1 4,398,433 + hfq b4172 0.39 +1.7 4,398,562 + hfq b4172 0.81 -1.6 4,398,565 + hfq b4172 0.82 +0.2 4,398,593 + +0.9 4,398,668 - +0.3 4,398,675 - -0.5 4,398,677 + -0.2 4,398,677 + +1.0 4,398,703 + -0.2 4,398,714 + +0.5 4,398,724 - +0.7 4,398,728 + +1.3 4,398,728 + +0.6 4,398,793 + +0.2 4,398,815 - -0.7 4,398,815 - +1.2 4,398,837 + hflX b4173 0.11 +0.3 4,398,926 - hflX b4173 0.18 +1.1 4,398,961 + hflX b4173 0.21 +0.4 4,398,961 + hflX b4173 0.21 +0.9 4,398,965 + hflX b4173 0.21 +0.3 4,398,965 + hflX b4173 0.21 +0.1 4,398,968 - hflX b4173 0.21 -0.1 4,398,983 - hflX b4173 0.22 -1.6 4,398,983 - hflX b4173 0.22 -1.3 4,398,992 - hflX b4173 0.23 +0.1 4,399,118 + hflX b4173 0.33 +0.7 4,399,140 + hflX b4173 0.35 +0.8 4,399,154 + hflX b4173 0.36 +1.1 4,399,217 - hflX b4173 0.41 -0.4 4,399,307 + hflX b4173 0.48 +0.4 4,399,307 + hflX b4173 0.48 +0.5 4,399,365 - hflX b4173 0.52 +0.2 4,399,365 - hflX b4173 0.52 -0.4 4,399,407 + hflX b4173 0.56 +1.9 4,399,407 + hflX b4173 0.56 +0.8 4,399,421 - hflX b4173 0.57 -0.0 4,399,449 - hflX b4173 0.59 +0.2 4,399,457 - hflX b4173 0.59 -0.2 4,399,457 - hflX b4173 0.59 +0.4 4,399,457 - hflX b4173 0.59 +0.1 4,399,500 - hflX b4173 0.63 -0.3 4,399,500 - hflX b4173 0.63 +1.3 4,399,542 + hflX b4173 0.66 +1.1 4,399,622 + hflX b4173 0.72 +0.9 4,399,691 + hflX b4173 0.78 +1.7 4,399,761 + hflX b4173 0.83 +1.2 4,399,793 - hflX b4173 0.86 -0.6 4,399,804 + hflX b4173 0.87 +0.7 4,400,041 + +1.0 4,400,041 + +0.5 4,400,043 - -0.3 4,400,126 + -1.0 4,400,162 + +0.5 4,400,162 + -0.2 4,400,293 - hflK b4174 0.18 -0.6 4,400,298 + hflK b4174 0.19 -0.4 4,400,298 + hflK b4174 0.19 +1.0 4,400,356 + hflK b4174 0.23 +0.1 4,400,385 - hflK b4174 0.26 +1.7 4,400,385 - hflK b4174 0.26 -1.7 4,400,411 + hflK b4174 0.28 +0.2 4,400,416 + hflK b4174 0.28 +1.0 4,400,416 + hflK b4174 0.28 -0.8 4,400,429 - hflK b4174 0.29 +0.0 4,400,476 - hflK b4174 0.33 +0.1 4,400,502 - hflK b4174 0.35 -0.6 4,400,534 - hflK b4174 0.38 +0.4 4,400,546 - hflK b4174 0.38 +0.3 4,400,555 - hflK b4174 0.39 +0.1 4,400,582 + hflK b4174 0.41 +0.3 4,400,586 + hflK b4174 0.42 +0.4 4,400,586 + hflK b4174 0.42 +0.1 4,400,620 - hflK b4174 0.44 +0.4 4,400,643 + hflK b4174 0.46 +0.3 4,400,654 + hflK b4174 0.47 +0.4 4,400,704 - hflK b4174 0.51 +0.0 4,400,704 - hflK b4174 0.51 -0.4 4,400,709 - hflK b4174 0.51 -1.4 4,400,716 + hflK b4174 0.52 -0.0 4,400,716 + hflK b4174 0.52 +0.7 4,400,747 + hflK b4174 0.54 -0.4 4,400,749 + hflK b4174 0.55 +0.7 4,400,767 - hflK b4174 0.56 +1.6 4,400,779 - hflK b4174 0.57 +0.3 4,400,779 - hflK b4174 0.57 +0.5 4,400,860 + hflK b4174 0.63 +0.7 4,400,875 - hflK b4174 0.65 +0.4 4,400,908 - hflK b4174 0.67 +0.3 4,400,932 + hflK b4174 0.69 -0.8 4,400,933 + hflK b4174 0.69 +0.2 4,400,942 + hflK b4174 0.70 +0.0 4,400,942 + hflK b4174 0.70 +0.4 4,400,942 - hflK b4174 0.70 +0.9 4,400,951 - hflK b4174 0.71 -0.6 4,400,951 - hflK b4174 0.71 -1.1 4,400,952 + hflK b4174 0.71 +0.3 4,400,952 + hflK b4174 0.71 -0.3 4,400,952 + hflK b4174 0.71 +0.5 4,400,966 + hflK b4174 0.72 +0.6
Or see this region's nucleotide sequence