Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pstA and pstC overlap by 1 nucleotides pstC and pstS are separated by 86 nucleotides
b3726: pstA - phosphate transporter subunit (NCBI), at 3,906,572 to 3,907,462
pstA
b3727: pstC - phosphate transporter subunit (NCBI), at 3,907,462 to 3,908,421
pstC
b3728: pstS - phosphate transporter subunit (NCBI), at 3,908,508 to 3,909,548
pstS
Position (kb)
3907
3908
3909 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3906.468 kb on - strand at 3906.483 kb on + strand at 3906.493 kb on - strand at 3906.493 kb on - strand at 3906.524 kb on - strand at 3906.526 kb on + strand at 3906.538 kb on + strand at 3906.538 kb on + strand at 3906.538 kb on + strand at 3906.538 kb on + strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.546 kb on - strand at 3906.574 kb on - strand at 3906.574 kb on - strand at 3906.579 kb on + strand at 3906.587 kb on - strand at 3906.587 kb on - strand at 3906.614 kb on + strand at 3906.642 kb on - strand at 3906.642 kb on - strand at 3906.644 kb on - strand at 3906.779 kb on - strand, within pstA at 3906.835 kb on - strand, within pstA at 3906.836 kb on - strand, within pstA at 3906.888 kb on - strand, within pstA at 3906.905 kb on - strand, within pstA at 3906.918 kb on + strand, within pstA at 3906.928 kb on - strand, within pstA at 3906.951 kb on - strand, within pstA at 3906.992 kb on - strand, within pstA at 3907.077 kb on - strand, within pstA at 3907.084 kb on - strand, within pstA at 3907.084 kb on - strand, within pstA at 3907.089 kb on - strand, within pstA at 3907.199 kb on - strand, within pstA at 3907.218 kb on - strand, within pstA at 3907.218 kb on - strand, within pstA at 3907.236 kb on - strand, within pstA at 3907.236 kb on - strand, within pstA at 3907.337 kb on - strand, within pstA at 3907.459 kb on - strand at 3907.474 kb on + strand at 3907.477 kb on + strand at 3907.489 kb on + strand at 3907.561 kb on - strand, within pstC at 3907.580 kb on - strand, within pstC at 3907.619 kb on - strand, within pstC at 3907.619 kb on - strand, within pstC at 3907.634 kb on + strand, within pstC at 3907.634 kb on + strand, within pstC at 3907.634 kb on + strand, within pstC at 3907.645 kb on - strand, within pstC at 3907.647 kb on - strand, within pstC at 3907.675 kb on - strand, within pstC at 3907.675 kb on - strand, within pstC at 3907.727 kb on - strand, within pstC at 3907.769 kb on - strand, within pstC at 3907.818 kb on - strand, within pstC at 3908.058 kb on - strand, within pstC at 3908.304 kb on - strand, within pstC at 3908.435 kb on + strand at 3908.469 kb on - strand at 3908.484 kb on - strand at 3908.484 kb on - strand at 3908.509 kb on - strand at 3908.524 kb on + strand at 3908.540 kb on - strand at 3908.567 kb on + strand at 3908.570 kb on + strand at 3908.570 kb on + strand at 3908.575 kb on - strand at 3908.580 kb on + strand at 3908.580 kb on + strand at 3908.588 kb on + strand at 3908.588 kb on + strand at 3908.648 kb on - strand, within pstS at 3908.665 kb on - strand, within pstS at 3908.708 kb on + strand, within pstS at 3908.724 kb on - strand, within pstS at 3908.749 kb on - strand, within pstS at 3908.749 kb on - strand, within pstS at 3908.891 kb on - strand, within pstS at 3908.919 kb on - strand, within pstS at 3908.973 kb on + strand, within pstS at 3908.997 kb on - strand, within pstS at 3909.029 kb on + strand, within pstS at 3909.095 kb on - strand, within pstS at 3909.155 kb on + strand, within pstS at 3909.161 kb on + strand, within pstS at 3909.231 kb on - strand, within pstS at 3909.231 kb on - strand, within pstS
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 3,906,468 - +0.3 3,906,483 + -0.1 3,906,493 - +0.8 3,906,493 - +1.2 3,906,524 - +0.0 3,906,526 + -0.6 3,906,538 + +0.8 3,906,538 + +2.0 3,906,538 + +0.0 3,906,538 + -0.8 3,906,546 - +1.2 3,906,546 - -0.7 3,906,546 - +0.6 3,906,546 - +0.3 3,906,546 - +0.8 3,906,574 - -0.1 3,906,574 - +0.1 3,906,579 + +0.4 3,906,587 - -0.4 3,906,587 - +0.8 3,906,614 + -0.4 3,906,642 - +0.2 3,906,642 - +0.4 3,906,644 - -0.1 3,906,779 - pstA b3726 0.23 -0.9 3,906,835 - pstA b3726 0.30 +0.2 3,906,836 - pstA b3726 0.30 +0.1 3,906,888 - pstA b3726 0.35 -1.0 3,906,905 - pstA b3726 0.37 -0.8 3,906,918 + pstA b3726 0.39 +0.2 3,906,928 - pstA b3726 0.40 -1.1 3,906,951 - pstA b3726 0.43 +0.3 3,906,992 - pstA b3726 0.47 -0.2 3,907,077 - pstA b3726 0.57 +0.6 3,907,084 - pstA b3726 0.57 -0.4 3,907,084 - pstA b3726 0.57 -1.4 3,907,089 - pstA b3726 0.58 +1.9 3,907,199 - pstA b3726 0.70 +0.2 3,907,218 - pstA b3726 0.73 -0.7 3,907,218 - pstA b3726 0.73 -0.1 3,907,236 - pstA b3726 0.75 +0.0 3,907,236 - pstA b3726 0.75 +0.9 3,907,337 - pstA b3726 0.86 -0.6 3,907,459 - -0.2 3,907,474 + +0.2 3,907,477 + +0.2 3,907,489 + +0.0 3,907,561 - pstC b3727 0.10 -0.3 3,907,580 - pstC b3727 0.12 -0.5 3,907,619 - pstC b3727 0.16 -1.3 3,907,619 - pstC b3727 0.16 +0.2 3,907,634 + pstC b3727 0.18 +1.2 3,907,634 + pstC b3727 0.18 +0.9 3,907,634 + pstC b3727 0.18 +1.2 3,907,645 - pstC b3727 0.19 -0.1 3,907,647 - pstC b3727 0.19 -0.3 3,907,675 - pstC b3727 0.22 -2.0 3,907,675 - pstC b3727 0.22 -0.4 3,907,727 - pstC b3727 0.28 +0.6 3,907,769 - pstC b3727 0.32 -1.7 3,907,818 - pstC b3727 0.37 +0.2 3,908,058 - pstC b3727 0.62 +0.6 3,908,304 - pstC b3727 0.88 -1.9 3,908,435 + +0.6 3,908,469 - +1.4 3,908,484 - -0.0 3,908,484 - +0.2 3,908,509 - -0.2 3,908,524 + +0.1 3,908,540 - -0.2 3,908,567 + +1.6 3,908,570 + +2.2 3,908,570 + -0.8 3,908,575 - -1.9 3,908,580 + +2.9 3,908,580 + +2.2 3,908,588 + +0.6 3,908,588 + +0.6 3,908,648 - pstS b3728 0.13 +1.3 3,908,665 - pstS b3728 0.15 +1.6 3,908,708 + pstS b3728 0.19 +3.2 3,908,724 - pstS b3728 0.21 +0.4 3,908,749 - pstS b3728 0.23 -0.1 3,908,749 - pstS b3728 0.23 -2.0 3,908,891 - pstS b3728 0.37 -0.8 3,908,919 - pstS b3728 0.39 -0.4 3,908,973 + pstS b3728 0.45 +0.6 3,908,997 - pstS b3728 0.47 -0.6 3,909,029 + pstS b3728 0.50 +1.2 3,909,095 - pstS b3728 0.56 -0.8 3,909,155 + pstS b3728 0.62 +0.0 3,909,161 + pstS b3728 0.63 +0.6 3,909,231 - pstS b3728 0.69 +0.2 3,909,231 - pstS b3728 0.69 -0.1
Or see this region's nucleotide sequence