Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaL and rfaK are separated by 31 nucleotides rfaK and rfaZ are separated by 32 nucleotides rfaZ and rfaY are separated by 70 nucleotides
b3622: rfaL - O-antigen ligase (NCBI), at 3,794,971 to 3,796,230
rfaL
b3623: rfaK - lipopolysaccharide core biosynthesis (NCBI), at 3,796,262 to 3,797,335
rfaK
b3624: rfaZ - lipopolysaccharide core biosynthesis protein (NCBI), at 3,797,368 to 3,798,219
rfaZ
b3625: rfaY - lipopolysaccharide core biosynthesis protein (NCBI), at 3,798,290 to 3,798,988
rfaY
Position (kb)
3796
3797
3798 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3795.275 kb on - strand, within rfaL at 3795.423 kb on + strand, within rfaL at 3795.423 kb on + strand, within rfaL at 3795.463 kb on + strand, within rfaL at 3795.474 kb on + strand, within rfaL at 3795.489 kb on + strand, within rfaL at 3795.489 kb on + strand, within rfaL at 3795.489 kb on + strand, within rfaL at 3795.494 kb on + strand, within rfaL at 3795.494 kb on + strand, within rfaL at 3795.514 kb on + strand, within rfaL at 3795.521 kb on - strand, within rfaL at 3795.522 kb on - strand, within rfaL at 3795.597 kb on + strand, within rfaL at 3795.609 kb on + strand, within rfaL at 3795.609 kb on + strand, within rfaL at 3795.627 kb on - strand, within rfaL at 3795.628 kb on - strand, within rfaL at 3795.706 kb on - strand, within rfaL at 3795.747 kb on - strand, within rfaL at 3795.773 kb on - strand, within rfaL at 3795.792 kb on - strand, within rfaL at 3795.792 kb on - strand, within rfaL at 3795.794 kb on + strand, within rfaL at 3795.801 kb on - strand, within rfaL at 3795.801 kb on - strand, within rfaL at 3795.818 kb on + strand, within rfaL at 3795.835 kb on - strand, within rfaL at 3795.835 kb on - strand, within rfaL at 3795.835 kb on - strand, within rfaL at 3795.835 kb on - strand, within rfaL at 3795.841 kb on - strand, within rfaL at 3795.893 kb on + strand, within rfaL at 3795.901 kb on - strand, within rfaL at 3795.901 kb on - strand, within rfaL at 3795.901 kb on - strand, within rfaL at 3795.936 kb on + strand, within rfaL at 3795.938 kb on + strand, within rfaL at 3795.939 kb on + strand, within rfaL at 3795.939 kb on + strand, within rfaL at 3795.939 kb on + strand, within rfaL at 3795.939 kb on + strand, within rfaL at 3795.939 kb on + strand, within rfaL at 3795.942 kb on - strand, within rfaL at 3795.943 kb on + strand, within rfaL at 3795.944 kb on + strand, within rfaL at 3795.944 kb on + strand, within rfaL at 3795.944 kb on + strand, within rfaL at 3795.944 kb on + strand, within rfaL at 3795.944 kb on + strand, within rfaL at 3795.945 kb on + strand, within rfaL at 3795.945 kb on + strand, within rfaL at 3795.950 kb on + strand, within rfaL at 3795.950 kb on + strand, within rfaL at 3795.955 kb on + strand, within rfaL at 3795.955 kb on + strand, within rfaL at 3795.970 kb on + strand, within rfaL at 3795.970 kb on + strand, within rfaL at 3795.971 kb on + strand, within rfaL at 3795.971 kb on + strand, within rfaL at 3795.978 kb on + strand, within rfaL at 3795.978 kb on - strand, within rfaL at 3795.981 kb on + strand, within rfaL at 3795.997 kb on - strand, within rfaL at 3795.997 kb on - strand, within rfaL at 3796.005 kb on + strand, within rfaL at 3796.015 kb on + strand, within rfaL at 3796.059 kb on + strand, within rfaL at 3796.107 kb on + strand at 3796.117 kb on + strand at 3796.117 kb on + strand at 3796.121 kb on + strand at 3796.122 kb on + strand at 3796.126 kb on + strand at 3796.130 kb on + strand at 3796.130 kb on + strand at 3796.131 kb on + strand at 3796.135 kb on + strand at 3796.135 kb on + strand at 3796.136 kb on + strand at 3796.139 kb on - strand at 3796.140 kb on + strand at 3796.140 kb on + strand at 3796.140 kb on + strand at 3796.140 kb on + strand at 3796.140 kb on + strand at 3796.140 kb on + strand at 3796.140 kb on + strand at 3796.142 kb on + strand at 3796.146 kb on - strand at 3796.149 kb on + strand at 3796.171 kb on + strand at 3796.171 kb on + strand at 3796.186 kb on + strand at 3796.261 kb on - strand at 3796.265 kb on + strand at 3796.296 kb on - strand at 3796.339 kb on - strand at 3796.339 kb on - strand at 3796.340 kb on - strand at 3796.346 kb on - strand at 3796.346 kb on - strand at 3796.405 kb on - strand, within rfaK at 3796.481 kb on - strand, within rfaK at 3796.526 kb on - strand, within rfaK at 3796.529 kb on - strand, within rfaK at 3796.529 kb on - strand, within rfaK at 3796.552 kb on - strand, within rfaK at 3796.667 kb on - strand, within rfaK at 3796.667 kb on - strand, within rfaK at 3796.701 kb on - strand, within rfaK at 3796.705 kb on - strand, within rfaK at 3796.830 kb on - strand, within rfaK at 3796.889 kb on - strand, within rfaK at 3796.955 kb on + strand, within rfaK at 3796.981 kb on - strand, within rfaK at 3796.981 kb on - strand, within rfaK at 3796.981 kb on - strand, within rfaK at 3796.991 kb on - strand, within rfaK at 3796.999 kb on - strand, within rfaK at 3796.999 kb on - strand, within rfaK at 3797.015 kb on - strand, within rfaK at 3797.015 kb on - strand, within rfaK at 3797.015 kb on - strand, within rfaK at 3797.015 kb on - strand, within rfaK at 3797.015 kb on - strand, within rfaK at 3797.015 kb on - strand, within rfaK at 3797.017 kb on - strand, within rfaK at 3797.025 kb on - strand, within rfaK at 3797.190 kb on - strand, within rfaK at 3797.190 kb on - strand, within rfaK at 3797.354 kb on - strand at 3797.441 kb on - strand at 3797.538 kb on - strand, within rfaZ at 3797.538 kb on - strand, within rfaZ at 3797.620 kb on - strand, within rfaZ at 3797.716 kb on - strand, within rfaZ at 3797.716 kb on - strand, within rfaZ at 3797.716 kb on - strand, within rfaZ at 3797.720 kb on - strand, within rfaZ at 3797.726 kb on - strand, within rfaZ at 3797.727 kb on - strand, within rfaZ at 3797.727 kb on - strand, within rfaZ at 3797.775 kb on + strand, within rfaZ at 3797.775 kb on + strand, within rfaZ at 3797.778 kb on + strand, within rfaZ at 3797.783 kb on - strand, within rfaZ at 3797.783 kb on - strand, within rfaZ at 3797.786 kb on - strand, within rfaZ at 3797.786 kb on - strand, within rfaZ at 3797.791 kb on - strand, within rfaZ at 3797.793 kb on + strand, within rfaZ at 3797.793 kb on + strand, within rfaZ at 3797.793 kb on - strand, within rfaZ at 3797.877 kb on - strand, within rfaZ at 3797.932 kb on - strand, within rfaZ at 3797.932 kb on - strand, within rfaZ at 3797.939 kb on - strand, within rfaZ at 3797.939 kb on - strand, within rfaZ at 3797.956 kb on - strand, within rfaZ at 3797.960 kb on - strand, within rfaZ at 3798.269 kb on - strand at 3798.277 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 3,795,275 - rfaL b3622 0.24 +2.1 3,795,423 + rfaL b3622 0.36 +2.1 3,795,423 + rfaL b3622 0.36 +2.1 3,795,463 + rfaL b3622 0.39 +2.1 3,795,474 + rfaL b3622 0.40 +2.1 3,795,489 + rfaL b3622 0.41 +2.1 3,795,489 + rfaL b3622 0.41 +2.1 3,795,489 + rfaL b3622 0.41 +2.1 3,795,494 + rfaL b3622 0.42 +2.1 3,795,494 + rfaL b3622 0.42 +2.1 3,795,514 + rfaL b3622 0.43 +2.1 3,795,521 - rfaL b3622 0.44 +2.1 3,795,522 - rfaL b3622 0.44 +2.1 3,795,597 + rfaL b3622 0.50 +2.1 3,795,609 + rfaL b3622 0.51 +2.1 3,795,609 + rfaL b3622 0.51 +2.1 3,795,627 - rfaL b3622 0.52 +2.1 3,795,628 - rfaL b3622 0.52 +2.1 3,795,706 - rfaL b3622 0.58 +2.1 3,795,747 - rfaL b3622 0.62 +2.1 3,795,773 - rfaL b3622 0.64 +2.1 3,795,792 - rfaL b3622 0.65 +2.1 3,795,792 - rfaL b3622 0.65 +2.1 3,795,794 + rfaL b3622 0.65 +2.1 3,795,801 - rfaL b3622 0.66 +2.7 3,795,801 - rfaL b3622 0.66 +0.5 3,795,818 + rfaL b3622 0.67 +2.1 3,795,835 - rfaL b3622 0.69 +2.1 3,795,835 - rfaL b3622 0.69 +2.1 3,795,835 - rfaL b3622 0.69 +2.1 3,795,835 - rfaL b3622 0.69 +2.1 3,795,841 - rfaL b3622 0.69 +1.1 3,795,893 + rfaL b3622 0.73 +2.1 3,795,901 - rfaL b3622 0.74 +2.1 3,795,901 - rfaL b3622 0.74 +1.1 3,795,901 - rfaL b3622 0.74 +2.1 3,795,936 + rfaL b3622 0.77 -0.5 3,795,938 + rfaL b3622 0.77 +2.1 3,795,939 + rfaL b3622 0.77 +0.5 3,795,939 + rfaL b3622 0.77 +0.9 3,795,939 + rfaL b3622 0.77 +1.5 3,795,939 + rfaL b3622 0.77 -0.9 3,795,939 + rfaL b3622 0.77 +2.1 3,795,942 - rfaL b3622 0.77 -0.5 3,795,943 + rfaL b3622 0.77 +1.1 3,795,944 + rfaL b3622 0.77 +1.1 3,795,944 + rfaL b3622 0.77 +2.1 3,795,944 + rfaL b3622 0.77 +1.1 3,795,944 + rfaL b3622 0.77 +2.7 3,795,944 + rfaL b3622 0.77 +2.1 3,795,945 + rfaL b3622 0.77 +1.7 3,795,945 + rfaL b3622 0.77 -1.3 3,795,950 + rfaL b3622 0.78 +0.1 3,795,950 + rfaL b3622 0.78 +0.1 3,795,955 + rfaL b3622 0.78 +2.1 3,795,955 + rfaL b3622 0.78 +1.1 3,795,970 + rfaL b3622 0.79 -0.9 3,795,970 + rfaL b3622 0.79 +0.5 3,795,971 + rfaL b3622 0.79 +1.1 3,795,971 + rfaL b3622 0.79 +0.5 3,795,978 + rfaL b3622 0.80 -1.6 3,795,978 - rfaL b3622 0.80 -1.2 3,795,981 + rfaL b3622 0.80 -1.5 3,795,997 - rfaL b3622 0.81 +2.1 3,795,997 - rfaL b3622 0.81 +2.1 3,796,005 + rfaL b3622 0.82 -0.4 3,796,015 + rfaL b3622 0.83 +1.3 3,796,059 + rfaL b3622 0.86 +0.6 3,796,107 + +0.2 3,796,117 + -0.0 3,796,117 + +0.5 3,796,121 + -0.5 3,796,122 + +0.1 3,796,126 + +1.7 3,796,130 + +0.3 3,796,130 + +0.4 3,796,131 + -0.4 3,796,135 + -0.1 3,796,135 + -0.1 3,796,136 + +0.4 3,796,139 - +1.1 3,796,140 + +1.3 3,796,140 + +0.4 3,796,140 + +1.3 3,796,140 + -1.2 3,796,140 + +0.5 3,796,140 + +1.1 3,796,140 + -0.2 3,796,142 + +0.2 3,796,146 - +2.1 3,796,149 + +0.4 3,796,171 + -1.8 3,796,171 + -0.1 3,796,186 + -0.8 3,796,261 - +2.1 3,796,265 + +2.1 3,796,296 - -0.7 3,796,339 - +0.2 3,796,339 - +0.3 3,796,340 - +0.1 3,796,346 - +0.3 3,796,346 - -0.7 3,796,405 - rfaK b3623 0.13 +1.1 3,796,481 - rfaK b3623 0.20 -0.4 3,796,526 - rfaK b3623 0.25 +0.5 3,796,529 - rfaK b3623 0.25 +2.1 3,796,529 - rfaK b3623 0.25 +2.1 3,796,552 - rfaK b3623 0.27 -1.6 3,796,667 - rfaK b3623 0.38 +1.1 3,796,667 - rfaK b3623 0.38 -0.7 3,796,701 - rfaK b3623 0.41 +2.1 3,796,705 - rfaK b3623 0.41 +2.1 3,796,830 - rfaK b3623 0.53 +2.1 3,796,889 - rfaK b3623 0.58 -0.2 3,796,955 + rfaK b3623 0.65 +2.1 3,796,981 - rfaK b3623 0.67 -0.2 3,796,981 - rfaK b3623 0.67 +1.1 3,796,981 - rfaK b3623 0.67 +0.5 3,796,991 - rfaK b3623 0.68 +1.1 3,796,999 - rfaK b3623 0.69 +0.5 3,796,999 - rfaK b3623 0.69 +2.1 3,797,015 - rfaK b3623 0.70 -1.5 3,797,015 - rfaK b3623 0.70 +2.1 3,797,015 - rfaK b3623 0.70 +0.9 3,797,015 - rfaK b3623 0.70 +0.1 3,797,015 - rfaK b3623 0.70 +0.3 3,797,015 - rfaK b3623 0.70 +0.5 3,797,017 - rfaK b3623 0.70 +0.1 3,797,025 - rfaK b3623 0.71 +0.3 3,797,190 - rfaK b3623 0.86 +2.1 3,797,190 - rfaK b3623 0.86 +0.3 3,797,354 - +2.1 3,797,441 - +1.0 3,797,538 - rfaZ b3624 0.20 -2.1 3,797,538 - rfaZ b3624 0.20 -1.1 3,797,620 - rfaZ b3624 0.30 +1.5 3,797,716 - rfaZ b3624 0.41 +1.7 3,797,716 - rfaZ b3624 0.41 +0.1 3,797,716 - rfaZ b3624 0.41 +2.1 3,797,720 - rfaZ b3624 0.41 +2.1 3,797,726 - rfaZ b3624 0.42 -0.2 3,797,727 - rfaZ b3624 0.42 +1.1 3,797,727 - rfaZ b3624 0.42 +1.5 3,797,775 + rfaZ b3624 0.48 +1.1 3,797,775 + rfaZ b3624 0.48 +0.6 3,797,778 + rfaZ b3624 0.48 -1.6 3,797,783 - rfaZ b3624 0.49 +0.9 3,797,783 - rfaZ b3624 0.49 -0.2 3,797,786 - rfaZ b3624 0.49 +0.1 3,797,786 - rfaZ b3624 0.49 +0.1 3,797,791 - rfaZ b3624 0.50 +1.7 3,797,793 + rfaZ b3624 0.50 +1.1 3,797,793 + rfaZ b3624 0.50 +0.5 3,797,793 - rfaZ b3624 0.50 -0.7 3,797,877 - rfaZ b3624 0.60 -0.7 3,797,932 - rfaZ b3624 0.66 +2.1 3,797,932 - rfaZ b3624 0.66 +1.1 3,797,939 - rfaZ b3624 0.67 +1.1 3,797,939 - rfaZ b3624 0.67 +1.1 3,797,956 - rfaZ b3624 0.69 +2.1 3,797,960 - rfaZ b3624 0.69 +1.1 3,798,269 - +0.5 3,798,277 - +0.4
Or see this region's nucleotide sequence