Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livF and livG are separated by 1 nucleotides livG and livM overlap by 4 nucleotides livM and livH overlap by 4 nucleotides livH and livK are separated by 47 nucleotides
b3454: livF - ATP-binding component of leucine transport (VIMSS), at 3,590,747 to 3,591,460
livF
b3455: livG - leucine/isoleucine/valine transporter subunit (NCBI), at 3,591,462 to 3,592,229
livG
b3456: livM - leucine/isoleucine/valine transporter subunit (NCBI), at 3,592,226 to 3,593,503
livM
b3457: livH - leucine/isoleucine/valine transporter subunit (NCBI), at 3,593,500 to 3,594,426
livH
b3458: livK - leucine transporter subunit (NCBI), at 3,594,474 to 3,595,583
livK
Position (kb)
3592
3593
3594 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3591.252 kb on + strand, within livF at 3591.252 kb on + strand, within livF at 3591.381 kb on - strand, within livF at 3591.381 kb on - strand, within livF at 3591.419 kb on - strand at 3591.479 kb on - strand at 3591.479 kb on - strand at 3591.547 kb on - strand, within livG at 3591.584 kb on + strand, within livG at 3591.592 kb on - strand, within livG at 3591.748 kb on - strand, within livG at 3591.748 kb on - strand, within livG at 3591.748 kb on - strand, within livG at 3591.765 kb on + strand, within livG at 3591.768 kb on - strand, within livG at 3591.834 kb on + strand, within livG at 3591.834 kb on + strand, within livG at 3591.881 kb on + strand, within livG at 3591.907 kb on + strand, within livG at 3591.937 kb on - strand, within livG at 3591.943 kb on - strand, within livG at 3591.962 kb on - strand, within livG at 3591.974 kb on - strand, within livG at 3592.053 kb on + strand, within livG at 3592.148 kb on - strand, within livG at 3592.229 kb on + strand at 3592.321 kb on - strand at 3592.436 kb on + strand, within livM at 3592.436 kb on + strand, within livM at 3592.590 kb on - strand, within livM at 3592.640 kb on + strand, within livM at 3592.763 kb on + strand, within livM at 3592.763 kb on + strand, within livM at 3592.768 kb on + strand, within livM at 3592.768 kb on + strand, within livM at 3592.822 kb on + strand, within livM at 3592.822 kb on + strand, within livM at 3592.834 kb on + strand, within livM at 3593.081 kb on + strand, within livM at 3593.081 kb on + strand, within livM at 3593.103 kb on - strand, within livM at 3593.103 kb on - strand, within livM at 3593.139 kb on + strand, within livM at 3593.210 kb on + strand, within livM at 3593.210 kb on + strand, within livM at 3593.244 kb on + strand, within livM at 3593.339 kb on + strand, within livM at 3593.375 kb on - strand, within livM at 3593.383 kb on - strand at 3593.383 kb on - strand at 3593.401 kb on + strand at 3593.409 kb on - strand at 3593.424 kb on - strand at 3593.438 kb on - strand at 3593.438 kb on - strand at 3593.524 kb on + strand at 3593.620 kb on - strand, within livH at 3593.620 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.655 kb on - strand, within livH at 3593.660 kb on + strand, within livH at 3593.660 kb on + strand, within livH at 3593.749 kb on + strand, within livH at 3593.825 kb on + strand, within livH at 3593.833 kb on - strand, within livH at 3593.869 kb on + strand, within livH at 3593.942 kb on + strand, within livH at 3593.942 kb on + strand, within livH at 3593.966 kb on - strand, within livH at 3594.055 kb on - strand, within livH at 3594.098 kb on - strand, within livH at 3594.150 kb on + strand, within livH at 3594.150 kb on + strand, within livH at 3594.220 kb on + strand, within livH at 3594.232 kb on - strand, within livH at 3594.232 kb on - strand, within livH at 3594.236 kb on + strand, within livH at 3594.236 kb on + strand, within livH at 3594.346 kb on - strand at 3594.350 kb on - strand at 3594.350 kb on - strand at 3594.404 kb on - strand at 3594.404 kb on - strand at 3594.417 kb on + strand at 3594.469 kb on + strand at 3594.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 3,591,252 + livF b3454 0.71 +0.6 3,591,252 + livF b3454 0.71 +1.6 3,591,381 - livF b3454 0.89 +1.2 3,591,381 - livF b3454 0.89 +0.7 3,591,419 - +1.1 3,591,479 - +0.5 3,591,479 - +0.8 3,591,547 - livG b3455 0.11 +0.8 3,591,584 + livG b3455 0.16 +2.6 3,591,592 - livG b3455 0.17 +0.9 3,591,748 - livG b3455 0.37 +1.7 3,591,748 - livG b3455 0.37 +2.0 3,591,748 - livG b3455 0.37 +1.7 3,591,765 + livG b3455 0.39 +1.5 3,591,768 - livG b3455 0.40 +1.5 3,591,834 + livG b3455 0.48 +1.7 3,591,834 + livG b3455 0.48 +2.1 3,591,881 + livG b3455 0.55 +1.4 3,591,907 + livG b3455 0.58 +2.9 3,591,937 - livG b3455 0.62 +1.5 3,591,943 - livG b3455 0.63 +1.1 3,591,962 - livG b3455 0.65 +1.3 3,591,974 - livG b3455 0.67 +0.7 3,592,053 + livG b3455 0.77 +1.5 3,592,148 - livG b3455 0.89 +1.1 3,592,229 + +1.1 3,592,321 - +1.1 3,592,436 + livM b3456 0.16 +1.3 3,592,436 + livM b3456 0.16 +1.0 3,592,590 - livM b3456 0.28 +1.5 3,592,640 + livM b3456 0.32 +0.8 3,592,763 + livM b3456 0.42 +0.5 3,592,763 + livM b3456 0.42 +2.2 3,592,768 + livM b3456 0.42 +1.5 3,592,768 + livM b3456 0.42 +1.2 3,592,822 + livM b3456 0.47 +1.8 3,592,822 + livM b3456 0.47 +1.4 3,592,834 + livM b3456 0.48 +1.7 3,593,081 + livM b3456 0.67 +1.5 3,593,081 + livM b3456 0.67 +1.2 3,593,103 - livM b3456 0.69 +1.5 3,593,103 - livM b3456 0.69 +1.8 3,593,139 + livM b3456 0.71 +1.1 3,593,210 + livM b3456 0.77 +1.6 3,593,210 + livM b3456 0.77 +1.4 3,593,244 + livM b3456 0.80 +1.1 3,593,339 + livM b3456 0.87 +1.3 3,593,375 - livM b3456 0.90 +1.5 3,593,383 - +0.4 3,593,383 - +0.9 3,593,401 + +1.8 3,593,409 - +1.4 3,593,424 - +0.4 3,593,438 - +1.7 3,593,438 - +1.0 3,593,524 + +1.6 3,593,620 - livH b3457 0.13 +0.7 3,593,620 - livH b3457 0.13 +1.1 3,593,655 - livH b3457 0.17 -0.3 3,593,655 - livH b3457 0.17 +1.7 3,593,655 - livH b3457 0.17 +1.0 3,593,655 - livH b3457 0.17 +1.6 3,593,660 + livH b3457 0.17 +1.7 3,593,660 + livH b3457 0.17 +0.7 3,593,749 + livH b3457 0.27 +1.3 3,593,825 + livH b3457 0.35 +1.0 3,593,833 - livH b3457 0.36 +1.7 3,593,869 + livH b3457 0.40 +1.7 3,593,942 + livH b3457 0.48 +1.6 3,593,942 + livH b3457 0.48 +1.3 3,593,966 - livH b3457 0.50 +1.3 3,594,055 - livH b3457 0.60 +1.0 3,594,098 - livH b3457 0.65 +1.6 3,594,150 + livH b3457 0.70 +0.9 3,594,150 + livH b3457 0.70 +1.4 3,594,220 + livH b3457 0.78 +1.1 3,594,232 - livH b3457 0.79 +1.3 3,594,232 - livH b3457 0.79 +1.8 3,594,236 + livH b3457 0.79 +1.2 3,594,236 + livH b3457 0.79 +1.4 3,594,346 - +1.2 3,594,350 - +1.2 3,594,350 - +0.6 3,594,404 - +0.8 3,594,404 - +1.2 3,594,417 + +2.5 3,594,469 + +1.6 3,594,485 - -2.3
Or see this region's nucleotide sequence