Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metC and yghB are separated by 139 nucleotides yghB and yqhC are separated by 39 nucleotides
b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445
metC
b3009: yghB - conserved inner membrane protein (NCBI), at 3,151,585 to 3,152,244
yghB
b3010: yqhC - putative ARAC-type regulatory protein (VIMSS), at 3,152,284 to 3,153,240
yqhC
Position (kb)
3151
3152
3153 Strain fitness (log2 ratio)
-1
0
1
2 at 3150.640 kb on - strand, within metC at 3150.654 kb on + strand, within metC at 3150.669 kb on + strand, within metC at 3150.706 kb on - strand, within metC at 3150.791 kb on - strand, within metC at 3150.820 kb on + strand, within metC at 3150.820 kb on + strand, within metC at 3150.912 kb on + strand, within metC at 3150.914 kb on + strand, within metC at 3150.915 kb on + strand, within metC at 3150.932 kb on - strand, within metC at 3151.015 kb on + strand at 3151.015 kb on + strand, within metC at 3151.028 kb on + strand, within metC at 3151.038 kb on - strand, within metC at 3151.038 kb on - strand, within metC at 3151.074 kb on - strand, within metC at 3151.074 kb on - strand, within metC at 3151.120 kb on + strand, within metC at 3151.125 kb on + strand, within metC at 3151.138 kb on - strand, within metC at 3151.216 kb on + strand, within metC at 3151.216 kb on + strand, within metC at 3151.237 kb on + strand, within metC at 3151.274 kb on - strand, within metC at 3151.376 kb on + strand at 3151.655 kb on - strand, within yghB at 3151.859 kb on + strand, within yghB at 3151.859 kb on + strand, within yghB at 3151.867 kb on - strand, within yghB at 3151.889 kb on + strand, within yghB at 3151.889 kb on + strand, within yghB at 3152.046 kb on + strand, within yghB at 3152.046 kb on + strand, within yghB at 3152.076 kb on + strand, within yghB at 3152.084 kb on - strand, within yghB at 3152.101 kb on - strand, within yghB at 3152.101 kb on - strand, within yghB at 3152.130 kb on + strand, within yghB at 3152.133 kb on + strand, within yghB at 3152.152 kb on - strand, within yghB at 3152.153 kb on - strand, within yghB at 3152.186 kb on + strand at 3152.256 kb on + strand at 3152.256 kb on + strand at 3152.280 kb on + strand at 3152.290 kb on + strand at 3152.290 kb on + strand at 3152.372 kb on + strand at 3152.377 kb on + strand at 3152.608 kb on - strand, within yqhC at 3152.608 kb on - strand, within yqhC at 3152.615 kb on + strand, within yqhC at 3152.615 kb on + strand, within yqhC at 3152.628 kb on - strand, within yqhC at 3152.630 kb on - strand, within yqhC at 3152.635 kb on - strand, within yqhC at 3152.728 kb on - strand, within yqhC at 3152.738 kb on - strand, within yqhC at 3152.837 kb on - strand, within yqhC at 3152.922 kb on - strand, within yqhC at 3153.056 kb on - strand, within yqhC
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 3,150,640 - metC b3008 0.32 +1.9 3,150,654 + metC b3008 0.33 +1.2 3,150,669 + metC b3008 0.35 +1.6 3,150,706 - metC b3008 0.38 +1.3 3,150,791 - metC b3008 0.45 +1.5 3,150,820 + metC b3008 0.47 +1.3 3,150,820 + metC b3008 0.47 +1.7 3,150,912 + metC b3008 0.55 +1.4 3,150,914 + metC b3008 0.55 +0.9 3,150,915 + metC b3008 0.55 -1.5 3,150,932 - metC b3008 0.57 +1.3 3,151,015 + +1.3 3,151,015 + metC b3008 0.64 +2.0 3,151,028 + metC b3008 0.65 +1.2 3,151,038 - metC b3008 0.66 +1.9 3,151,038 - metC b3008 0.66 +0.8 3,151,074 - metC b3008 0.69 +1.4 3,151,074 - metC b3008 0.69 +1.0 3,151,120 + metC b3008 0.73 +0.9 3,151,125 + metC b3008 0.73 +0.9 3,151,138 - metC b3008 0.74 +1.7 3,151,216 + metC b3008 0.81 +1.2 3,151,216 + metC b3008 0.81 +1.4 3,151,237 + metC b3008 0.82 +1.1 3,151,274 - metC b3008 0.86 +1.1 3,151,376 + +1.4 3,151,655 - yghB b3009 0.11 +1.8 3,151,859 + yghB b3009 0.42 +0.5 3,151,859 + yghB b3009 0.42 -0.6 3,151,867 - yghB b3009 0.43 +0.5 3,151,889 + yghB b3009 0.46 +0.2 3,151,889 + yghB b3009 0.46 +0.1 3,152,046 + yghB b3009 0.70 -0.8 3,152,046 + yghB b3009 0.70 +0.2 3,152,076 + yghB b3009 0.74 -0.1 3,152,084 - yghB b3009 0.76 +0.5 3,152,101 - yghB b3009 0.78 +0.4 3,152,101 - yghB b3009 0.78 +1.1 3,152,130 + yghB b3009 0.83 +0.7 3,152,133 + yghB b3009 0.83 +1.3 3,152,152 - yghB b3009 0.86 +0.6 3,152,153 - yghB b3009 0.86 +0.2 3,152,186 + +0.5 3,152,256 + +0.0 3,152,256 + +0.8 3,152,280 + -0.3 3,152,290 + +1.5 3,152,290 + -0.1 3,152,372 + +0.4 3,152,377 + -0.8 3,152,608 - yqhC b3010 0.34 +1.0 3,152,608 - yqhC b3010 0.34 +1.2 3,152,615 + yqhC b3010 0.35 +0.4 3,152,615 + yqhC b3010 0.35 -0.1 3,152,628 - yqhC b3010 0.36 +0.3 3,152,630 - yqhC b3010 0.36 +0.2 3,152,635 - yqhC b3010 0.37 -0.5 3,152,728 - yqhC b3010 0.46 -0.0 3,152,738 - yqhC b3010 0.47 -1.0 3,152,837 - yqhC b3010 0.58 -0.1 3,152,922 - yqhC b3010 0.67 +0.2 3,153,056 - yqhC b3010 0.81 -0.4
Or see this region's nucleotide sequence