Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygaZ and ygaH overlap by 11 nucleotides ygaH and mprA are separated by 90 nucleotides mprA and emrA are separated by 126 nucleotides
b2682: ygaZ - predicted transporter (NCBI), at 2,807,639 to 2,808,376
ygaZ
b2683: ygaH - predicted inner membrane protein (NCBI), at 2,808,366 to 2,808,701
ygaH
b2684: mprA - DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI), at 2,808,792 to 2,809,322
mprA
b2685: emrA - multidrug efflux system (NCBI), at 2,809,449 to 2,810,621
emrA
Position (kb)
2808
2809
2810 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2807.853 kb on - strand, within ygaZ at 2807.917 kb on - strand, within ygaZ at 2807.917 kb on - strand, within ygaZ at 2807.919 kb on - strand, within ygaZ at 2808.115 kb on - strand, within ygaZ at 2808.115 kb on - strand, within ygaZ at 2808.190 kb on + strand, within ygaZ at 2808.190 kb on + strand, within ygaZ at 2808.198 kb on - strand, within ygaZ at 2808.322 kb on - strand at 2808.322 kb on - strand at 2808.379 kb on - strand at 2808.379 kb on - strand at 2808.399 kb on - strand at 2808.515 kb on + strand, within ygaH at 2808.517 kb on - strand, within ygaH at 2808.533 kb on + strand, within ygaH at 2808.533 kb on + strand, within ygaH at 2808.534 kb on - strand, within ygaH at 2808.534 kb on - strand, within ygaH at 2808.560 kb on - strand, within ygaH at 2808.565 kb on - strand at 2808.645 kb on + strand, within ygaH at 2808.645 kb on + strand, within ygaH at 2808.645 kb on + strand, within ygaH at 2808.648 kb on - strand, within ygaH at 2808.655 kb on + strand, within ygaH at 2808.658 kb on - strand, within ygaH at 2808.660 kb on - strand, within ygaH at 2808.662 kb on + strand, within ygaH at 2808.662 kb on + strand, within ygaH at 2808.671 kb on - strand at 2808.876 kb on - strand, within mprA at 2809.004 kb on - strand, within mprA at 2809.100 kb on + strand, within mprA at 2809.100 kb on + strand, within mprA at 2809.107 kb on + strand, within mprA at 2809.200 kb on + strand, within mprA at 2809.287 kb on + strand at 2809.433 kb on - strand at 2809.497 kb on + strand at 2809.497 kb on + strand at 2809.546 kb on - strand at 2809.548 kb on - strand at 2809.748 kb on - strand, within emrA at 2809.748 kb on - strand, within emrA at 2809.806 kb on - strand, within emrA at 2809.931 kb on - strand, within emrA at 2810.041 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.100 kb on - strand, within emrA at 2810.100 kb on - strand, within emrA at 2810.103 kb on - strand, within emrA at 2810.127 kb on - strand, within emrA at 2810.147 kb on - strand, within emrA at 2810.204 kb on - strand, within emrA at 2810.320 kb on + strand, within emrA
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 2,807,853 - ygaZ b2682 0.29 -0.6 2,807,917 - ygaZ b2682 0.38 +0.5 2,807,917 - ygaZ b2682 0.38 +0.1 2,807,919 - ygaZ b2682 0.38 -0.6 2,808,115 - ygaZ b2682 0.64 -0.4 2,808,115 - ygaZ b2682 0.64 -1.7 2,808,190 + ygaZ b2682 0.75 +0.1 2,808,190 + ygaZ b2682 0.75 -1.3 2,808,198 - ygaZ b2682 0.76 +0.4 2,808,322 - -0.7 2,808,322 - +0.0 2,808,379 - +0.8 2,808,379 - +0.4 2,808,399 - -0.7 2,808,515 + ygaH b2683 0.44 -0.4 2,808,517 - ygaH b2683 0.45 -0.2 2,808,533 + ygaH b2683 0.50 -0.2 2,808,533 + ygaH b2683 0.50 -0.9 2,808,534 - ygaH b2683 0.50 -0.1 2,808,534 - ygaH b2683 0.50 +0.3 2,808,560 - ygaH b2683 0.58 -0.2 2,808,565 - -2.1 2,808,645 + ygaH b2683 0.83 +0.2 2,808,645 + ygaH b2683 0.83 -0.4 2,808,645 + ygaH b2683 0.83 -0.4 2,808,648 - ygaH b2683 0.84 -1.1 2,808,655 + ygaH b2683 0.86 -0.1 2,808,658 - ygaH b2683 0.87 -0.6 2,808,660 - ygaH b2683 0.88 -0.4 2,808,662 + ygaH b2683 0.88 -0.2 2,808,662 + ygaH b2683 0.88 +0.3 2,808,671 - -0.2 2,808,876 - mprA b2684 0.16 +1.4 2,809,004 - mprA b2684 0.40 +1.4 2,809,100 + mprA b2684 0.58 +2.1 2,809,100 + mprA b2684 0.58 +0.1 2,809,107 + mprA b2684 0.59 +0.1 2,809,200 + mprA b2684 0.77 +0.8 2,809,287 + +0.1 2,809,433 - +0.6 2,809,497 + +0.5 2,809,497 + -1.0 2,809,546 - -0.2 2,809,548 - -0.6 2,809,748 - emrA b2685 0.25 -0.2 2,809,748 - emrA b2685 0.25 +1.1 2,809,806 - emrA b2685 0.30 -0.1 2,809,931 - emrA b2685 0.41 -0.2 2,810,041 + emrA b2685 0.50 -1.3 2,810,097 + emrA b2685 0.55 +0.2 2,810,097 + emrA b2685 0.55 -0.0 2,810,097 + emrA b2685 0.55 +0.6 2,810,100 - emrA b2685 0.55 +0.4 2,810,100 - emrA b2685 0.55 +0.5 2,810,103 - emrA b2685 0.56 +0.4 2,810,127 - emrA b2685 0.58 +0.4 2,810,147 - emrA b2685 0.60 +0.3 2,810,204 - emrA b2685 0.64 +0.1 2,810,320 + emrA b2685 0.74 -0.0
Or see this region's nucleotide sequence