Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfjQ and yfjR are separated by 216 nucleotides yfjR and ypjK are separated by 40 nucleotides ypjK and b2636 overlap by 1 nucleotides b2636 and yfjT are separated by 23 nucleotides
b2633: yfjQ - CP4-57 prophage; predicted protein (NCBI), at 2,766,687 to 2,767,508
yfjQ
b2634: yfjR - CP4-57 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 2,767,725 to 2,768,426
yfjR
b2635: ypjK - CP4-57 prophage; predicted inner membrane protein (NCBI), at 2,768,467 to 2,768,703
ypjK
b2636: b2636 - orf, hypothetical protein (VIMSS), at 2,768,703 to 2,769,146
b2636
b2637: yfjT - CP4-57 prophage; predicted protein (NCBI), at 2,769,170 to 2,769,637
yfjT
Position (kb)
2767
2768
2769 Strain fitness (log2 ratio)
-1
0
1 at 2766.741 kb on + strand at 2766.779 kb on - strand, within yfjQ at 2766.816 kb on + strand, within yfjQ at 2766.905 kb on - strand, within yfjQ at 2767.046 kb on - strand, within yfjQ at 2767.051 kb on - strand, within yfjQ at 2767.051 kb on - strand, within yfjQ at 2767.051 kb on - strand, within yfjQ at 2767.055 kb on + strand, within yfjQ at 2767.134 kb on - strand, within yfjQ at 2767.150 kb on - strand, within yfjQ at 2767.229 kb on - strand, within yfjQ at 2767.328 kb on - strand, within yfjQ at 2767.342 kb on - strand, within yfjQ at 2767.342 kb on - strand, within yfjQ at 2767.420 kb on + strand, within yfjQ at 2767.519 kb on + strand at 2767.563 kb on + strand at 2767.605 kb on + strand at 2767.649 kb on + strand at 2767.658 kb on - strand at 2767.904 kb on + strand, within yfjR at 2768.029 kb on - strand, within yfjR at 2768.215 kb on + strand, within yfjR at 2768.258 kb on - strand, within yfjR at 2768.258 kb on - strand, within yfjR at 2768.388 kb on - strand at 2768.429 kb on - strand at 2768.429 kb on - strand at 2768.437 kb on + strand at 2768.445 kb on - strand at 2768.460 kb on - strand at 2768.470 kb on - strand at 2768.582 kb on - strand, within ypjK at 2768.582 kb on - strand, within ypjK at 2768.582 kb on - strand, within ypjK at 2768.791 kb on - strand, within b2636 at 2768.810 kb on + strand, within b2636 at 2768.843 kb on - strand, within b2636 at 2768.845 kb on - strand, within b2636 at 2768.919 kb on - strand, within b2636 at 2768.941 kb on - strand, within b2636 at 2768.941 kb on - strand, within b2636 at 2769.051 kb on - strand, within b2636 at 2769.064 kb on - strand, within b2636 at 2769.064 kb on - strand, within b2636 at 2769.099 kb on + strand, within b2636 at 2769.099 kb on + strand, within b2636 at 2769.118 kb on - strand at 2769.242 kb on - strand, within yfjT at 2769.288 kb on - strand, within yfjT
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 2,766,741 + +0.3 2,766,779 - yfjQ b2633 0.11 -0.3 2,766,816 + yfjQ b2633 0.16 -0.6 2,766,905 - yfjQ b2633 0.27 -0.2 2,767,046 - yfjQ b2633 0.44 +0.3 2,767,051 - yfjQ b2633 0.44 +0.1 2,767,051 - yfjQ b2633 0.44 +0.4 2,767,051 - yfjQ b2633 0.44 -0.7 2,767,055 + yfjQ b2633 0.45 -0.4 2,767,134 - yfjQ b2633 0.54 -0.1 2,767,150 - yfjQ b2633 0.56 -0.7 2,767,229 - yfjQ b2633 0.66 +1.4 2,767,328 - yfjQ b2633 0.78 +0.6 2,767,342 - yfjQ b2633 0.80 -0.4 2,767,342 - yfjQ b2633 0.80 -0.6 2,767,420 + yfjQ b2633 0.89 -0.0 2,767,519 + +1.0 2,767,563 + +1.4 2,767,605 + -0.0 2,767,649 + +0.6 2,767,658 - +0.4 2,767,904 + yfjR b2634 0.25 +0.0 2,768,029 - yfjR b2634 0.43 -0.3 2,768,215 + yfjR b2634 0.70 +1.4 2,768,258 - yfjR b2634 0.76 +0.8 2,768,258 - yfjR b2634 0.76 +0.5 2,768,388 - -0.6 2,768,429 - +0.1 2,768,429 - -0.6 2,768,437 + +0.2 2,768,445 - -0.5 2,768,460 - -0.5 2,768,470 - -0.1 2,768,582 - ypjK b2635 0.49 -1.3 2,768,582 - ypjK b2635 0.49 -0.9 2,768,582 - ypjK b2635 0.49 -0.3 2,768,791 - b2636 b2636 0.20 +0.2 2,768,810 + b2636 b2636 0.24 -0.0 2,768,843 - b2636 b2636 0.32 +0.1 2,768,845 - b2636 b2636 0.32 +0.3 2,768,919 - b2636 b2636 0.49 +0.0 2,768,941 - b2636 b2636 0.54 +0.7 2,768,941 - b2636 b2636 0.54 +0.9 2,769,051 - b2636 b2636 0.78 -0.2 2,769,064 - b2636 b2636 0.81 +0.6 2,769,064 - b2636 b2636 0.81 -0.5 2,769,099 + b2636 b2636 0.89 -0.1 2,769,099 + b2636 b2636 0.89 +0.6 2,769,118 - +0.2 2,769,242 - yfjT b2637 0.15 +0.0 2,769,288 - yfjT b2637 0.25 +0.4
Or see this region's nucleotide sequence