Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pssA and yfiM are separated by 45 nucleotides yfiM and kgtP overlap by 4 nucleotides kgtP and rrfG are separated by 322 nucleotides rrfG and rrlG are separated by 92 nucleotides
b2585: pssA - phosphatidylserine synthase; phospholipid synthesis (VIMSS), at 2,720,749 to 2,722,104
pssA
b2586: yfiM - predicted protein (RefSeq), at 2,722,150 to 2,722,473
yfiM
b2587: kgtP - alpha-ketoglutarate transporter (NCBI), at 2,722,470 to 2,723,768
kgtP
b2588: rrfG - 5S ribosomal RNA (NCBI), at 2,724,091 to 2,724,210
rrfG
b2589: rrlG - 23S ribosomal RNA (NCBI), at 2,724,303 to 2,727,206
rrlG
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-1
0
1
2 at 2722.110 kb on + strand at 2722.113 kb on - strand at 2722.115 kb on + strand at 2722.171 kb on + strand at 2722.227 kb on + strand, within yfiM at 2722.332 kb on + strand, within yfiM at 2722.387 kb on - strand, within yfiM at 2722.415 kb on + strand, within yfiM at 2722.435 kb on + strand, within yfiM at 2722.539 kb on - strand at 2722.539 kb on - strand at 2722.557 kb on + strand at 2722.557 kb on + strand at 2722.570 kb on + strand at 2722.604 kb on - strand, within kgtP at 2722.604 kb on - strand, within kgtP at 2722.633 kb on + strand, within kgtP at 2722.641 kb on - strand, within kgtP at 2722.783 kb on + strand, within kgtP at 2722.801 kb on + strand, within kgtP at 2722.943 kb on + strand, within kgtP at 2722.943 kb on + strand, within kgtP at 2723.060 kb on - strand, within kgtP at 2723.060 kb on - strand, within kgtP at 2723.145 kb on + strand, within kgtP at 2723.210 kb on - strand, within kgtP at 2723.235 kb on + strand, within kgtP at 2723.348 kb on - strand, within kgtP at 2723.408 kb on - strand, within kgtP at 2723.418 kb on + strand, within kgtP at 2723.418 kb on + strand, within kgtP at 2723.436 kb on + strand, within kgtP at 2723.457 kb on - strand, within kgtP at 2723.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 2,722,110 + +0.4 2,722,113 - -1.0 2,722,115 + +1.0 2,722,171 + -0.2 2,722,227 + yfiM b2586 0.24 +0.5 2,722,332 + yfiM b2586 0.56 +0.7 2,722,387 - yfiM b2586 0.73 +0.4 2,722,415 + yfiM b2586 0.82 +0.6 2,722,435 + yfiM b2586 0.88 +0.7 2,722,539 - +0.0 2,722,539 - +1.0 2,722,557 + -0.1 2,722,557 + +0.3 2,722,570 + -0.8 2,722,604 - kgtP b2587 0.10 -0.4 2,722,604 - kgtP b2587 0.10 -0.2 2,722,633 + kgtP b2587 0.13 +0.3 2,722,641 - kgtP b2587 0.13 +0.6 2,722,783 + kgtP b2587 0.24 +0.1 2,722,801 + kgtP b2587 0.25 +0.7 2,722,943 + kgtP b2587 0.36 -0.6 2,722,943 + kgtP b2587 0.36 +0.9 2,723,060 - kgtP b2587 0.45 +0.4 2,723,060 - kgtP b2587 0.45 +0.3 2,723,145 + kgtP b2587 0.52 +1.8 2,723,210 - kgtP b2587 0.57 +0.4 2,723,235 + kgtP b2587 0.59 +0.7 2,723,348 - kgtP b2587 0.68 +1.0 2,723,408 - kgtP b2587 0.72 +0.7 2,723,418 + kgtP b2587 0.73 +0.6 2,723,418 + kgtP b2587 0.73 +0.9 2,723,436 + kgtP b2587 0.74 +1.0 2,723,457 - kgtP b2587 0.76 +0.5 2,723,698 - +0.9
Or see this region's nucleotide sequence