Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intZ and yffL are separated by 190 nucleotides yffL and yffM are separated by 469 nucleotides yffM and yffN are separated by 11 nucleotides
b2442: intZ - CPZ-55 prophage; predicted integrase (RefSeq), at 2,556,880 to 2,558,088
intZ
b2443: yffL - CPZ-55 prophage; predicted protein (NCBI), at 2,558,279 to 2,558,920
yffL
b2444: yffM - CPZ-55 prophage; predicted protein (NCBI), at 2,559,390 to 2,559,635
yffM
b2445: yffN - CPZ-55 prophage; predicted protein (RefSeq), at 2,559,647 to 2,560,015
yffN
Position (kb)
2558
2559 Strain fitness (log2 ratio)
-1
0
1
2 at 2557.406 kb on - strand, within intZ at 2557.475 kb on - strand, within intZ at 2557.764 kb on + strand, within intZ at 2557.808 kb on - strand, within intZ at 2557.864 kb on + strand, within intZ at 2558.028 kb on - strand at 2558.342 kb on + strand at 2558.504 kb on + strand, within yffL at 2558.504 kb on + strand, within yffL at 2558.561 kb on + strand, within yffL at 2558.678 kb on + strand, within yffL at 2558.734 kb on + strand, within yffL at 2558.749 kb on - strand, within yffL at 2558.754 kb on - strand, within yffL at 2558.812 kb on + strand, within yffL at 2558.941 kb on - strand at 2559.256 kb on + strand at 2559.410 kb on - strand at 2559.410 kb on - strand at 2559.536 kb on - strand, within yffM at 2559.536 kb on - strand, within yffM at 2559.699 kb on + strand, within yffN at 2559.794 kb on - strand, within yffN at 2559.794 kb on - strand, within yffN at 2559.897 kb on + strand, within yffN at 2559.897 kb on + strand, within yffN at 2559.915 kb on - strand, within yffN
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 2,557,406 - intZ b2442 0.44 +0.2 2,557,475 - intZ b2442 0.49 +0.8 2,557,764 + intZ b2442 0.73 +1.0 2,557,808 - intZ b2442 0.77 +0.3 2,557,864 + intZ b2442 0.81 +0.3 2,558,028 - -0.8 2,558,342 + +0.7 2,558,504 + yffL b2443 0.35 +0.5 2,558,504 + yffL b2443 0.35 +0.6 2,558,561 + yffL b2443 0.44 -0.1 2,558,678 + yffL b2443 0.62 +0.7 2,558,734 + yffL b2443 0.71 -0.0 2,558,749 - yffL b2443 0.73 +1.2 2,558,754 - yffL b2443 0.74 +2.1 2,558,812 + yffL b2443 0.83 -0.0 2,558,941 - +0.9 2,559,256 + +0.2 2,559,410 - -0.8 2,559,410 - +0.5 2,559,536 - yffM b2444 0.59 -0.1 2,559,536 - yffM b2444 0.59 +0.9 2,559,699 + yffN b2445 0.14 +0.0 2,559,794 - yffN b2445 0.40 +0.9 2,559,794 - yffN b2445 0.40 +0.2 2,559,897 + yffN b2445 0.68 -1.0 2,559,897 + yffN b2445 0.68 +0.2 2,559,915 - yffN b2445 0.73 +0.8
Or see this region's nucleotide sequence