Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysW and cysU overlap by 1 nucleotides cysU and cysP overlap by 1 nucleotides cysP and ucpA are separated by 303 nucleotides
b2423: cysW - sulfate/thiosulfate transporter subunit (NCBI), at 2,538,826 to 2,539,701
cysW
b2424: cysU - sulfate/thiosulfate transporter subunit (NCBI), at 2,539,701 to 2,540,534
cysU
b2425: cysP - thiosulfate transporter subunit (NCBI), at 2,540,534 to 2,541,550
cysP
b2426: ucpA - putative oxidoreductase (VIMSS), at 2,541,854 to 2,542,645
ucpA
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-1
0
1
2 at 2539.694 kb on - strand at 2540.062 kb on + strand, within cysU at 2540.110 kb on - strand, within cysU at 2540.110 kb on - strand, within cysU at 2540.129 kb on + strand, within cysU at 2540.492 kb on + strand at 2540.535 kb on + strand at 2540.563 kb on - strand at 2540.677 kb on - strand, within cysP at 2540.677 kb on - strand, within cysP at 2540.695 kb on - strand, within cysP at 2540.752 kb on - strand, within cysP at 2540.816 kb on - strand, within cysP at 2540.858 kb on + strand, within cysP at 2540.872 kb on + strand, within cysP at 2541.064 kb on + strand, within cysP at 2541.066 kb on + strand, within cysP at 2541.077 kb on - strand, within cysP at 2541.159 kb on + strand, within cysP at 2541.181 kb on - strand, within cysP at 2541.194 kb on + strand, within cysP at 2541.229 kb on - strand, within cysP at 2541.421 kb on - strand, within cysP at 2541.621 kb on + strand at 2541.699 kb on + strand at 2541.733 kb on + strand at 2542.113 kb on + strand, within ucpA at 2542.215 kb on - strand, within ucpA at 2542.432 kb on - strand, within ucpA
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 2,539,694 - -0.7 2,540,062 + cysU b2424 0.43 +2.3 2,540,110 - cysU b2424 0.49 +0.4 2,540,110 - cysU b2424 0.49 +0.5 2,540,129 + cysU b2424 0.51 +0.7 2,540,492 + +1.1 2,540,535 + +0.9 2,540,563 - +0.8 2,540,677 - cysP b2425 0.14 +1.0 2,540,677 - cysP b2425 0.14 +0.3 2,540,695 - cysP b2425 0.16 +1.4 2,540,752 - cysP b2425 0.21 +1.3 2,540,816 - cysP b2425 0.28 +1.2 2,540,858 + cysP b2425 0.32 +1.3 2,540,872 + cysP b2425 0.33 +0.4 2,541,064 + cysP b2425 0.52 +1.6 2,541,066 + cysP b2425 0.52 +1.7 2,541,077 - cysP b2425 0.53 +1.4 2,541,159 + cysP b2425 0.61 +0.8 2,541,181 - cysP b2425 0.64 +1.2 2,541,194 + cysP b2425 0.65 +1.2 2,541,229 - cysP b2425 0.68 +1.9 2,541,421 - cysP b2425 0.87 +1.3 2,541,621 + +0.3 2,541,699 + +0.5 2,541,733 + -1.3 2,542,113 + ucpA b2426 0.33 +0.8 2,542,215 - ucpA b2426 0.46 +0.7 2,542,432 - ucpA b2426 0.73 +0.1
Or see this region's nucleotide sequence