Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcC and yfcD are separated by 32 nucleotides yfcD and yfcE are separated by 57 nucleotides yfcE and yfcF are separated by 52 nucleotides yfcF and yfcG are separated by 135 nucleotides
b2298: yfcC - putative S-transferase (VIMSS), at 2,415,103 to 2,416,623
yfcC
b2299: yfcD - predicted NUDIX hydrolase (NCBI), at 2,416,656 to 2,417,198
yfcD
b2300: yfcE - orf, hypothetical protein (VIMSS), at 2,417,256 to 2,417,810
yfcE
b2301: yfcF - predicted enzyme (NCBI), at 2,417,863 to 2,418,507
yfcF
b2302: yfcG - predicted glutathione S-transferase (NCBI), at 2,418,643 to 2,419,290
yfcG
Position (kb)
2417
2418 Strain fitness (log2 ratio)
-1
0
1 at 2416.315 kb on - strand, within yfcC at 2416.315 kb on - strand, within yfcC at 2416.355 kb on + strand, within yfcC at 2416.358 kb on - strand, within yfcC at 2416.358 kb on - strand, within yfcC at 2416.420 kb on - strand, within yfcC at 2416.456 kb on + strand, within yfcC at 2416.475 kb on + strand at 2416.502 kb on + strand at 2416.507 kb on + strand at 2416.512 kb on + strand at 2416.512 kb on + strand at 2416.516 kb on - strand at 2416.532 kb on - strand at 2416.532 kb on - strand at 2416.552 kb on + strand at 2416.605 kb on + strand at 2416.606 kb on - strand at 2416.609 kb on + strand at 2416.736 kb on + strand, within yfcD at 2416.811 kb on - strand, within yfcD at 2416.829 kb on + strand, within yfcD at 2416.973 kb on + strand, within yfcD at 2416.977 kb on - strand, within yfcD at 2416.977 kb on - strand, within yfcD at 2416.977 kb on - strand, within yfcD at 2417.064 kb on + strand, within yfcD at 2417.124 kb on + strand, within yfcD at 2417.124 kb on + strand, within yfcD at 2417.162 kb on - strand at 2417.177 kb on + strand at 2417.225 kb on + strand at 2417.235 kb on - strand at 2417.408 kb on + strand, within yfcE at 2417.730 kb on + strand, within yfcE at 2417.745 kb on + strand, within yfcE at 2417.745 kb on + strand, within yfcE at 2417.753 kb on - strand, within yfcE at 2417.753 kb on - strand, within yfcE at 2417.759 kb on - strand at 2417.965 kb on + strand, within yfcF at 2417.965 kb on + strand, within yfcF at 2417.970 kb on - strand, within yfcF at 2417.970 kb on - strand, within yfcF at 2418.048 kb on - strand, within yfcF at 2418.133 kb on + strand, within yfcF at 2418.153 kb on + strand, within yfcF at 2418.184 kb on - strand, within yfcF at 2418.247 kb on + strand, within yfcF at 2418.247 kb on + strand, within yfcF at 2418.269 kb on + strand, within yfcF at 2418.269 kb on + strand, within yfcF at 2418.445 kb on - strand at 2418.572 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.607 kb on - strand at 2418.714 kb on + strand, within yfcG at 2418.736 kb on + strand, within yfcG at 2418.739 kb on - strand, within yfcG at 2418.739 kb on - strand, within yfcG at 2418.744 kb on - strand, within yfcG
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 2,416,315 - yfcC b2298 0.80 +0.5 2,416,315 - yfcC b2298 0.80 -0.1 2,416,355 + yfcC b2298 0.82 +0.8 2,416,358 - yfcC b2298 0.83 +0.6 2,416,358 - yfcC b2298 0.83 -0.1 2,416,420 - yfcC b2298 0.87 +0.9 2,416,456 + yfcC b2298 0.89 -0.6 2,416,475 + -0.2 2,416,502 + -0.6 2,416,507 + -0.3 2,416,512 + +0.0 2,416,512 + +0.4 2,416,516 - +0.1 2,416,532 - +0.5 2,416,532 - +0.1 2,416,552 + -0.5 2,416,605 + +0.5 2,416,606 - -0.2 2,416,609 + -0.1 2,416,736 + yfcD b2299 0.15 +0.8 2,416,811 - yfcD b2299 0.29 +0.8 2,416,829 + yfcD b2299 0.32 +0.9 2,416,973 + yfcD b2299 0.58 +0.6 2,416,977 - yfcD b2299 0.59 -1.0 2,416,977 - yfcD b2299 0.59 +1.0 2,416,977 - yfcD b2299 0.59 +0.4 2,417,064 + yfcD b2299 0.75 -0.3 2,417,124 + yfcD b2299 0.86 +0.6 2,417,124 + yfcD b2299 0.86 -0.2 2,417,162 - +1.1 2,417,177 + +0.7 2,417,225 + +0.3 2,417,235 - -0.0 2,417,408 + yfcE b2300 0.27 +1.2 2,417,730 + yfcE b2300 0.85 +0.0 2,417,745 + yfcE b2300 0.88 +0.5 2,417,745 + yfcE b2300 0.88 -0.3 2,417,753 - yfcE b2300 0.90 +0.6 2,417,753 - yfcE b2300 0.90 +0.8 2,417,759 - -0.1 2,417,965 + yfcF b2301 0.16 +0.4 2,417,965 + yfcF b2301 0.16 +0.4 2,417,970 - yfcF b2301 0.17 +0.1 2,417,970 - yfcF b2301 0.17 -0.2 2,418,048 - yfcF b2301 0.29 -0.5 2,418,133 + yfcF b2301 0.42 -0.8 2,418,153 + yfcF b2301 0.45 +0.4 2,418,184 - yfcF b2301 0.50 +0.2 2,418,247 + yfcF b2301 0.60 -0.4 2,418,247 + yfcF b2301 0.60 -1.7 2,418,269 + yfcF b2301 0.63 -0.6 2,418,269 + yfcF b2301 0.63 -1.0 2,418,445 - -0.2 2,418,572 - +1.0 2,418,607 - +0.2 2,418,607 - +0.8 2,418,607 - +0.5 2,418,607 - -0.4 2,418,714 + yfcG b2302 0.11 -0.8 2,418,736 + yfcG b2302 0.14 +0.7 2,418,739 - yfcG b2302 0.15 -0.1 2,418,739 - yfcG b2302 0.15 +0.0 2,418,744 - yfcG b2302 0.16 -0.3
Or see this region's nucleotide sequence