Strain Fitness in Escherichia coli BW25113 around b2049

Experiment: 48 hr rep2_Tiberius_no_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpsG and cpsB are separated by 104 nucleotidescpsB and wcaI are separated by 2 nucleotides b2048: cpsG - phosphomannomutase (NCBI), at 2,119,633 to 2,121,003 cpsG b2049: cpsB - mannose-1-phosphate guanyltransferase (NCBI), at 2,121,108 to 2,122,544 cpsB b2050: wcaI - predicted glycosyl transferase (NCBI), at 2,122,547 to 2,123,770 wcaI Position (kb) 2121 2122 2123Strain fitness (log2 ratio) -1 0 1 2at 2120.189 kb on + strand, within cpsGat 2120.628 kb on - strand, within cpsGat 2120.645 kb on - strand, within cpsGat 2120.645 kb on - strand, within cpsGat 2120.689 kb on + strand, within cpsGat 2120.759 kb on + strand, within cpsGat 2120.759 kb on + strand, within cpsGat 2120.782 kb on - strand, within cpsGat 2120.814 kb on + strand, within cpsGat 2120.823 kb on - strand, within cpsGat 2120.880 kb on - strandat 2120.880 kb on - strandat 2121.026 kb on + strandat 2121.026 kb on + strandat 2121.027 kb on + strandat 2121.354 kb on + strand, within cpsBat 2121.354 kb on + strand, within cpsBat 2121.452 kb on + strand, within cpsBat 2121.452 kb on + strand, within cpsBat 2121.504 kb on + strand, within cpsBat 2121.605 kb on + strand, within cpsBat 2121.742 kb on + strand, within cpsBat 2121.742 kb on + strand, within cpsBat 2121.969 kb on + strand, within cpsBat 2122.061 kb on + strand, within cpsBat 2122.061 kb on + strand, within cpsBat 2122.748 kb on + strand, within wcaIat 2122.748 kb on + strand, within wcaIat 2123.290 kb on + strand, within wcaIat 2123.290 kb on + strand, within wcaIat 2123.479 kb on + strand, within wcaI

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Per-strain Table

Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution
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2,120,189 + cpsG b2048 0.41 +0.5
2,120,628 - cpsG b2048 0.73 -0.2
2,120,645 - cpsG b2048 0.74 +0.3
2,120,645 - cpsG b2048 0.74 +0.6
2,120,689 + cpsG b2048 0.77 -0.1
2,120,759 + cpsG b2048 0.82 -0.2
2,120,759 + cpsG b2048 0.82 +1.9
2,120,782 - cpsG b2048 0.84 +0.4
2,120,814 + cpsG b2048 0.86 +0.1
2,120,823 - cpsG b2048 0.87 -1.3
2,120,880 - +0.2
2,120,880 - -1.0
2,121,026 + -0.6
2,121,026 + +1.5
2,121,027 + +0.3
2,121,354 + cpsB b2049 0.17 +0.9
2,121,354 + cpsB b2049 0.17 +0.6
2,121,452 + cpsB b2049 0.24 +0.4
2,121,452 + cpsB b2049 0.24 +0.6
2,121,504 + cpsB b2049 0.28 +0.4
2,121,605 + cpsB b2049 0.35 +1.4
2,121,742 + cpsB b2049 0.44 +0.3
2,121,742 + cpsB b2049 0.44 +0.4
2,121,969 + cpsB b2049 0.60 +1.2
2,122,061 + cpsB b2049 0.66 +0.3
2,122,061 + cpsB b2049 0.66 +0.6
2,122,748 + wcaI b2050 0.16 +0.4
2,122,748 + wcaI b2050 0.16 -0.3
2,123,290 + wcaI b2050 0.61 -1.3
2,123,290 + wcaI b2050 0.61 +0.1
2,123,479 + wcaI b2050 0.76 +0.4

Or see this region's nucleotide sequence