Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt erfK and cobT are separated by 64 nucleotides cobT and cobS are separated by 11 nucleotides cobS and cobU overlap by 4 nucleotides
b1990: erfK - conserved protein with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,060,415 to 2,061,347
erfK
b1991: cobT - nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (NCBI), at 2,061,412 to 2,062,491
cobT
b1992: cobS - cobalamin synthase (NCBI), at 2,062,503 to 2,063,246
cobS
b1993: cobU - adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI), at 2,063,243 to 2,063,788
cobU
Position (kb)
2061
2062
2063 Strain fitness (log2 ratio)
-1
0
1 at 2060.520 kb on + strand, within erfK at 2060.520 kb on + strand, within erfK at 2060.530 kb on - strand, within erfK at 2060.536 kb on + strand, within erfK at 2060.573 kb on + strand, within erfK at 2060.610 kb on - strand, within erfK at 2060.630 kb on - strand, within erfK at 2060.630 kb on - strand, within erfK at 2060.715 kb on + strand, within erfK at 2060.730 kb on + strand, within erfK at 2060.730 kb on - strand, within erfK at 2060.743 kb on - strand, within erfK at 2060.743 kb on - strand, within erfK at 2060.821 kb on - strand, within erfK at 2060.976 kb on - strand, within erfK at 2060.976 kb on - strand, within erfK at 2060.983 kb on + strand, within erfK at 2060.989 kb on - strand, within erfK at 2061.007 kb on - strand, within erfK at 2061.078 kb on - strand, within erfK at 2061.200 kb on - strand, within erfK at 2061.211 kb on - strand, within erfK at 2061.216 kb on - strand, within erfK at 2061.365 kb on - strand at 2061.365 kb on - strand at 2061.426 kb on - strand at 2061.426 kb on - strand at 2061.517 kb on + strand at 2061.734 kb on + strand, within cobT at 2061.734 kb on + strand, within cobT at 2061.871 kb on + strand, within cobT at 2061.871 kb on + strand, within cobT at 2061.890 kb on - strand, within cobT at 2061.955 kb on - strand, within cobT at 2061.955 kb on - strand, within cobT at 2061.974 kb on + strand, within cobT at 2062.013 kb on - strand, within cobT at 2062.105 kb on + strand, within cobT at 2062.134 kb on - strand, within cobT at 2062.134 kb on - strand, within cobT at 2062.238 kb on - strand, within cobT at 2062.238 kb on - strand, within cobT at 2062.333 kb on + strand, within cobT at 2062.333 kb on + strand, within cobT at 2062.357 kb on - strand, within cobT at 2062.416 kb on - strand at 2062.704 kb on + strand, within cobS at 2063.068 kb on + strand, within cobS at 2063.182 kb on + strand at 2063.182 kb on - strand at 2063.203 kb on - strand at 2063.378 kb on - strand, within cobU at 2063.378 kb on - strand, within cobU at 2063.390 kb on + strand, within cobU
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 2,060,520 + erfK b1990 0.11 -0.4 2,060,520 + erfK b1990 0.11 +0.0 2,060,530 - erfK b1990 0.12 -0.0 2,060,536 + erfK b1990 0.13 +0.5 2,060,573 + erfK b1990 0.17 -0.2 2,060,610 - erfK b1990 0.21 +0.5 2,060,630 - erfK b1990 0.23 -0.3 2,060,630 - erfK b1990 0.23 -0.3 2,060,715 + erfK b1990 0.32 -0.1 2,060,730 + erfK b1990 0.34 +0.3 2,060,730 - erfK b1990 0.34 +0.2 2,060,743 - erfK b1990 0.35 -0.1 2,060,743 - erfK b1990 0.35 +0.3 2,060,821 - erfK b1990 0.44 +0.9 2,060,976 - erfK b1990 0.60 -0.6 2,060,976 - erfK b1990 0.60 +0.8 2,060,983 + erfK b1990 0.61 -0.7 2,060,989 - erfK b1990 0.62 -0.2 2,061,007 - erfK b1990 0.63 +0.1 2,061,078 - erfK b1990 0.71 +0.0 2,061,200 - erfK b1990 0.84 -0.3 2,061,211 - erfK b1990 0.85 +1.2 2,061,216 - erfK b1990 0.86 -0.4 2,061,365 - -0.4 2,061,365 - +0.6 2,061,426 - +0.3 2,061,426 - -0.1 2,061,517 + +0.9 2,061,734 + cobT b1991 0.30 -0.7 2,061,734 + cobT b1991 0.30 -1.4 2,061,871 + cobT b1991 0.42 +0.1 2,061,871 + cobT b1991 0.42 -0.2 2,061,890 - cobT b1991 0.44 -0.1 2,061,955 - cobT b1991 0.50 +1.6 2,061,955 - cobT b1991 0.50 +1.7 2,061,974 + cobT b1991 0.52 -0.1 2,062,013 - cobT b1991 0.56 +1.2 2,062,105 + cobT b1991 0.64 -0.3 2,062,134 - cobT b1991 0.67 +0.4 2,062,134 - cobT b1991 0.67 +1.4 2,062,238 - cobT b1991 0.76 +0.7 2,062,238 - cobT b1991 0.76 +1.5 2,062,333 + cobT b1991 0.85 +0.0 2,062,333 + cobT b1991 0.85 +0.5 2,062,357 - cobT b1991 0.88 +0.5 2,062,416 - +1.0 2,062,704 + cobS b1992 0.27 -0.0 2,063,068 + cobS b1992 0.76 +0.1 2,063,182 + +0.1 2,063,182 - +0.8 2,063,203 - +0.5 2,063,378 - cobU b1993 0.25 +0.6 2,063,378 - cobU b1993 0.25 +0.0 2,063,390 + cobU b1993 0.27 +0.5
Or see this region's nucleotide sequence