Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and uvrY overlap by 4 nucleotides uvrY and yecF are separated by 458 nucleotides yecF and sdiA are separated by 67 nucleotides
b1913: uvrC - excinuclease ABC, subunit C; repair of UV damage to DNA (VIMSS), at 1,990,898 to 1,992,730
uvrC
b1914: uvrY - response regulator (NCBI), at 1,992,727 to 1,993,383
uvrY
b1915: yecF - hypothetical protein (NCBI), at 1,993,842 to 1,994,066
yecF
b1916: sdiA - DNA-binding transcriptional activator (NCBI), at 1,994,134 to 1,994,856
sdiA
Position (kb)
1992
1993
1994 Strain fitness (log2 ratio)
-1
0
1
2 at 1991.798 kb on - strand, within uvrC at 1991.798 kb on - strand, within uvrC at 1991.857 kb on + strand, within uvrC at 1991.922 kb on + strand, within uvrC at 1991.948 kb on - strand, within uvrC at 1992.150 kb on + strand, within uvrC at 1992.202 kb on + strand, within uvrC at 1992.227 kb on - strand, within uvrC at 1992.461 kb on - strand, within uvrC at 1992.461 kb on - strand, within uvrC at 1992.523 kb on - strand, within uvrC at 1992.523 kb on - strand, within uvrC at 1992.721 kb on + strand at 1992.862 kb on + strand, within uvrY at 1993.136 kb on - strand, within uvrY at 1993.136 kb on - strand, within uvrY at 1993.220 kb on - strand, within uvrY at 1993.236 kb on + strand, within uvrY at 1993.236 kb on + strand, within uvrY at 1993.244 kb on - strand, within uvrY at 1993.244 kb on - strand, within uvrY at 1993.268 kb on - strand, within uvrY at 1993.309 kb on + strand, within uvrY at 1993.317 kb on + strand, within uvrY at 1993.328 kb on + strand at 1993.345 kb on + strand at 1993.350 kb on + strand at 1993.350 kb on + strand at 1993.358 kb on - strand at 1993.415 kb on - strand at 1993.419 kb on + strand at 1993.419 kb on - strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.534 kb on + strand at 1993.537 kb on + strand at 1993.540 kb on - strand at 1993.542 kb on - strand at 1993.548 kb on - strand at 1993.548 kb on - strand at 1993.549 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.560 kb on + strand at 1993.560 kb on + strand at 1993.565 kb on + strand at 1993.571 kb on + strand at 1993.571 kb on + strand at 1993.571 kb on + strand at 1993.717 kb on + strand at 1993.717 kb on + strand at 1993.771 kb on + strand at 1993.771 kb on + strand at 1993.771 kb on + strand at 1993.772 kb on + strand at 1993.775 kb on + strand at 1993.775 kb on + strand at 1993.775 kb on + strand at 1993.777 kb on + strand at 1993.777 kb on + strand at 1993.777 kb on + strand at 1993.777 kb on + strand at 1993.786 kb on - strand at 1993.864 kb on - strand at 1993.864 kb on - strand at 1993.894 kb on - strand, within yecF at 1993.903 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.922 kb on - strand, within yecF at 1993.922 kb on - strand, within yecF at 1993.928 kb on + strand, within yecF at 1993.929 kb on - strand, within yecF at 1993.934 kb on + strand, within yecF at 1993.934 kb on + strand, within yecF at 1993.937 kb on + strand, within yecF at 1993.961 kb on + strand, within yecF at 1993.961 kb on + strand, within yecF at 1994.001 kb on + strand, within yecF at 1994.015 kb on - strand, within yecF at 1994.100 kb on + strand at 1994.110 kb on - strand at 1994.110 kb on - strand at 1994.120 kb on - strand at 1994.154 kb on - strand at 1994.154 kb on - strand at 1994.155 kb on - strand at 1994.249 kb on + strand, within sdiA at 1994.262 kb on + strand, within sdiA at 1994.268 kb on - strand, within sdiA at 1994.283 kb on - strand, within sdiA at 1994.332 kb on + strand, within sdiA at 1994.344 kb on - strand, within sdiA at 1994.344 kb on - strand, within sdiA at 1994.345 kb on - strand, within sdiA at 1994.357 kb on - strand, within sdiA at 1994.357 kb on - strand, within sdiA at 1994.364 kb on + strand, within sdiA at 1994.367 kb on - strand, within sdiA at 1994.367 kb on - strand, within sdiA at 1994.367 kb on - strand, within sdiA at 1994.378 kb on - strand, within sdiA at 1994.378 kb on - strand, within sdiA at 1994.381 kb on - strand, within sdiA
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 1,991,798 - uvrC b1913 0.49 +0.5 1,991,798 - uvrC b1913 0.49 +1.1 1,991,857 + uvrC b1913 0.52 +0.1 1,991,922 + uvrC b1913 0.56 -0.1 1,991,948 - uvrC b1913 0.57 +0.3 1,992,150 + uvrC b1913 0.68 +0.5 1,992,202 + uvrC b1913 0.71 -0.1 1,992,227 - uvrC b1913 0.73 -1.0 1,992,461 - uvrC b1913 0.85 -0.3 1,992,461 - uvrC b1913 0.85 -0.8 1,992,523 - uvrC b1913 0.89 -1.2 1,992,523 - uvrC b1913 0.89 -0.5 1,992,721 + -0.6 1,992,862 + uvrY b1914 0.21 +1.3 1,993,136 - uvrY b1914 0.62 +0.7 1,993,136 - uvrY b1914 0.62 +0.7 1,993,220 - uvrY b1914 0.75 +0.6 1,993,236 + uvrY b1914 0.77 -0.6 1,993,236 + uvrY b1914 0.77 -0.2 1,993,244 - uvrY b1914 0.79 +1.0 1,993,244 - uvrY b1914 0.79 +1.8 1,993,268 - uvrY b1914 0.82 +0.5 1,993,309 + uvrY b1914 0.89 -0.4 1,993,317 + uvrY b1914 0.90 +0.3 1,993,328 + +0.0 1,993,345 + -0.8 1,993,350 + -0.7 1,993,350 + -0.7 1,993,358 - +1.0 1,993,415 - -0.1 1,993,419 + -0.1 1,993,419 - +0.7 1,993,522 + +0.7 1,993,522 + -0.2 1,993,522 + -0.6 1,993,522 + -0.3 1,993,534 + -0.8 1,993,537 + +0.8 1,993,540 - -0.1 1,993,542 - +1.9 1,993,548 - +0.9 1,993,548 - +0.9 1,993,549 + -0.5 1,993,559 + -0.5 1,993,559 + +0.4 1,993,559 + -1.2 1,993,559 + +0.6 1,993,559 + -0.2 1,993,559 + -0.3 1,993,560 + +0.4 1,993,560 + +0.1 1,993,565 + +0.5 1,993,571 + +1.3 1,993,571 + -0.0 1,993,571 + +0.8 1,993,717 + +1.4 1,993,717 + +0.9 1,993,771 + -0.1 1,993,771 + +0.6 1,993,771 + +1.1 1,993,772 + -0.6 1,993,775 + +1.4 1,993,775 + +0.9 1,993,775 + +1.9 1,993,777 + -0.0 1,993,777 + +0.7 1,993,777 + -0.1 1,993,777 + +0.0 1,993,786 - +0.9 1,993,864 - +0.6 1,993,864 - -0.7 1,993,894 - yecF b1915 0.23 -0.0 1,993,903 + yecF b1915 0.27 -0.1 1,993,921 + yecF b1915 0.35 +0.5 1,993,921 + yecF b1915 0.35 +0.0 1,993,921 + yecF b1915 0.35 +0.6 1,993,921 + yecF b1915 0.35 +0.7 1,993,922 - yecF b1915 0.36 -0.4 1,993,922 - yecF b1915 0.36 -0.9 1,993,928 + yecF b1915 0.38 -0.1 1,993,929 - yecF b1915 0.39 -0.5 1,993,934 + yecF b1915 0.41 -0.8 1,993,934 + yecF b1915 0.41 +0.3 1,993,937 + yecF b1915 0.42 +0.0 1,993,961 + yecF b1915 0.53 -1.1 1,993,961 + yecF b1915 0.53 +0.2 1,994,001 + yecF b1915 0.71 -0.7 1,994,015 - yecF b1915 0.77 +0.3 1,994,100 + +0.3 1,994,110 - +0.6 1,994,110 - -0.4 1,994,120 - -0.5 1,994,154 - -0.5 1,994,154 - -0.8 1,994,155 - +0.3 1,994,249 + sdiA b1916 0.16 -0.3 1,994,262 + sdiA b1916 0.18 -0.1 1,994,268 - sdiA b1916 0.19 -0.3 1,994,283 - sdiA b1916 0.21 +0.1 1,994,332 + sdiA b1916 0.27 +0.9 1,994,344 - sdiA b1916 0.29 -1.1 1,994,344 - sdiA b1916 0.29 -0.6 1,994,345 - sdiA b1916 0.29 +1.0 1,994,357 - sdiA b1916 0.31 +0.5 1,994,357 - sdiA b1916 0.31 -0.0 1,994,364 + sdiA b1916 0.32 +0.3 1,994,367 - sdiA b1916 0.32 -1.5 1,994,367 - sdiA b1916 0.32 -1.1 1,994,367 - sdiA b1916 0.32 +0.8 1,994,378 - sdiA b1916 0.34 +0.4 1,994,378 - sdiA b1916 0.34 +0.5 1,994,381 - sdiA b1916 0.34 +0.7
Or see this region's nucleotide sequence