Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gapA and yeaD are separated by 83 nucleotides yeaD and yeaE are separated by 47 nucleotides yeaE and mipA are separated by 89 nucleotides
b1779: gapA - glyceraldehyde-3-phosphate dehydrogenase (NCBI), at 1,860,795 to 1,861,790
gapA
b1780: yeaD - orf, hypothetical protein (VIMSS), at 1,861,874 to 1,862,758
yeaD
b1781: yeaE - predicted oxidoreductase (NCBI), at 1,862,806 to 1,863,660
yeaE
b1782: mipA - scaffolding protein for murein synthesizing machinery (NCBI), at 1,863,750 to 1,864,496
mipA
Position (kb)
1861
1862
1863 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1861.874 kb on - strand at 1861.874 kb on - strand at 1861.922 kb on + strand at 1862.020 kb on - strand, within yeaD at 1862.020 kb on - strand, within yeaD at 1862.069 kb on - strand, within yeaD at 1862.153 kb on + strand, within yeaD at 1862.434 kb on + strand, within yeaD at 1862.442 kb on - strand, within yeaD at 1862.545 kb on + strand, within yeaD at 1862.658 kb on - strand, within yeaD at 1862.691 kb on - strand at 1862.801 kb on + strand at 1862.801 kb on + strand at 1862.851 kb on - strand at 1862.851 kb on - strand at 1862.985 kb on - strand, within yeaE at 1863.088 kb on - strand, within yeaE at 1863.147 kb on + strand, within yeaE at 1863.391 kb on - strand, within yeaE at 1863.454 kb on + strand, within yeaE at 1863.511 kb on + strand, within yeaE at 1863.597 kb on - strand at 1863.603 kb on - strand at 1863.609 kb on - strand at 1863.609 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 1,861,874 - +0.5 1,861,874 - +0.6 1,861,922 + -0.5 1,862,020 - yeaD b1780 0.16 +0.7 1,862,020 - yeaD b1780 0.16 -0.5 1,862,069 - yeaD b1780 0.22 +0.4 1,862,153 + yeaD b1780 0.32 -0.2 1,862,434 + yeaD b1780 0.63 +1.5 1,862,442 - yeaD b1780 0.64 +1.4 1,862,545 + yeaD b1780 0.76 +0.0 1,862,658 - yeaD b1780 0.89 +0.5 1,862,691 - +0.2 1,862,801 + +1.8 1,862,801 + +0.2 1,862,851 - +0.5 1,862,851 - +1.1 1,862,985 - yeaE b1781 0.21 +0.3 1,863,088 - yeaE b1781 0.33 +0.7 1,863,147 + yeaE b1781 0.40 +0.3 1,863,391 - yeaE b1781 0.68 +0.7 1,863,454 + yeaE b1781 0.76 +0.8 1,863,511 + yeaE b1781 0.82 +0.1 1,863,597 - +0.5 1,863,603 - -0.3 1,863,609 - -1.8 1,863,609 - +1.5
Or see this region's nucleotide sequence