Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhC and cfa are separated by 290 nucleotides cfa and ribC are separated by 39 nucleotides ribC and mdtK are separated by 214 nucleotides
b1660: ydhC - predicted transporter (NCBI), at 1,737,935 to 1,739,146
ydhC
b1661: cfa - cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI), at 1,739,437 to 1,740,585
cfa
b1662: ribC - riboflavin synthase subunit alpha (NCBI), at 1,740,625 to 1,741,266
ribC
b1663: mdtK - multidrug efflux protein NorM (NCBI), at 1,741,481 to 1,742,854
mdtK
Position (kb)
1739
1740
1741 Strain fitness (log2 ratio)
-1
0
1 at 1738.723 kb on - strand, within ydhC at 1738.723 kb on - strand, within ydhC at 1738.896 kb on - strand, within ydhC at 1738.945 kb on - strand, within ydhC at 1738.945 kb on - strand, within ydhC at 1738.966 kb on - strand, within ydhC at 1739.263 kb on + strand at 1739.431 kb on - strand at 1739.431 kb on - strand at 1739.458 kb on - strand at 1739.473 kb on - strand at 1739.487 kb on + strand at 1739.797 kb on + strand, within cfa at 1740.226 kb on - strand, within cfa at 1740.389 kb on + strand, within cfa at 1740.447 kb on + strand, within cfa at 1740.447 kb on + strand, within cfa at 1740.449 kb on + strand, within cfa at 1740.449 kb on + strand, within cfa at 1740.505 kb on - strand at 1740.538 kb on + strand at 1741.305 kb on + strand at 1741.305 kb on + strand at 1741.328 kb on - strand at 1741.333 kb on - strand at 1741.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 1,738,723 - ydhC b1660 0.65 -0.9 1,738,723 - ydhC b1660 0.65 +1.1 1,738,896 - ydhC b1660 0.79 -0.0 1,738,945 - ydhC b1660 0.83 -0.0 1,738,945 - ydhC b1660 0.83 +0.8 1,738,966 - ydhC b1660 0.85 -0.0 1,739,263 + +0.1 1,739,431 - +1.0 1,739,431 - +0.1 1,739,458 - +0.8 1,739,473 - -0.1 1,739,487 + +0.6 1,739,797 + cfa b1661 0.31 +0.1 1,740,226 - cfa b1661 0.69 +0.1 1,740,389 + cfa b1661 0.83 +0.5 1,740,447 + cfa b1661 0.88 +0.3 1,740,447 + cfa b1661 0.88 +1.6 1,740,449 + cfa b1661 0.88 +0.5 1,740,449 + cfa b1661 0.88 +0.4 1,740,505 - +1.7 1,740,538 + -0.5 1,741,305 + -0.1 1,741,305 + +0.5 1,741,328 - +0.1 1,741,333 - +0.0 1,741,342 + -0.7
Or see this region's nucleotide sequence