Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speG and ynfC are separated by 2 nucleotides ynfC and b1586 are separated by 107 nucleotides b1586 and ynfE are separated by 198 nucleotides
b1584: speG - spermidine N1-acetyltransferase (NCBI), at 1,654,208 to 1,654,768
speG
b1585: ynfC - orf, hypothetical protein (VIMSS), at 1,654,771 to 1,655,481
ynfC
b1586: b1586 - orf, hypothetical protein (VIMSS), at 1,655,589 to 1,655,894
b1586
b1587: ynfE - oxidoreductase subunit (NCBI), at 1,656,093 to 1,658,519
ynfE
Position (kb)
1655
1656 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1654.690 kb on + strand, within speG at 1654.690 kb on + strand, within speG at 1654.711 kb on + strand, within speG at 1654.711 kb on + strand, within speG at 1654.719 kb on - strand at 1654.723 kb on + strand at 1654.723 kb on + strand at 1654.731 kb on - strand at 1654.745 kb on + strand at 1654.745 kb on + strand at 1654.753 kb on - strand at 1654.799 kb on + strand at 1654.807 kb on - strand at 1654.807 kb on - strand at 1654.812 kb on - strand at 1654.812 kb on - strand at 1655.022 kb on - strand, within ynfC at 1655.058 kb on + strand, within ynfC at 1655.077 kb on - strand, within ynfC at 1655.077 kb on - strand, within ynfC at 1655.102 kb on + strand, within ynfC at 1655.114 kb on + strand, within ynfC at 1655.123 kb on - strand, within ynfC at 1655.123 kb on - strand, within ynfC at 1655.147 kb on - strand, within ynfC at 1655.175 kb on - strand, within ynfC at 1655.199 kb on - strand, within ynfC at 1655.208 kb on + strand, within ynfC at 1655.216 kb on - strand, within ynfC at 1655.394 kb on + strand, within ynfC at 1655.402 kb on - strand at 1655.402 kb on - strand, within ynfC at 1655.413 kb on - strand at 1655.428 kb on + strand at 1655.500 kb on - strand at 1655.500 kb on - strand at 1655.626 kb on + strand, within b1586 at 1655.634 kb on - strand, within b1586 at 1655.643 kb on + strand, within b1586 at 1655.645 kb on + strand, within b1586 at 1655.655 kb on + strand, within b1586 at 1655.655 kb on + strand, within b1586 at 1655.786 kb on - strand, within b1586 at 1655.786 kb on - strand, within b1586 at 1655.841 kb on + strand, within b1586 at 1655.858 kb on + strand, within b1586 at 1655.955 kb on - strand at 1656.025 kb on - strand at 1656.291 kb on - strand at 1656.291 kb on - strand at 1656.418 kb on - strand, within ynfE at 1656.569 kb on - strand, within ynfE at 1656.673 kb on - strand, within ynfE at 1656.712 kb on - strand, within ynfE at 1656.712 kb on - strand, within ynfE at 1656.800 kb on + strand, within ynfE at 1656.800 kb on + strand, within ynfE
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 1,654,690 + speG b1584 0.86 +0.5 1,654,690 + speG b1584 0.86 +0.2 1,654,711 + speG b1584 0.90 -1.6 1,654,711 + speG b1584 0.90 -1.1 1,654,719 - +0.5 1,654,723 + +0.8 1,654,723 + +0.5 1,654,731 - +0.8 1,654,745 + -2.1 1,654,745 + -0.2 1,654,753 - -0.3 1,654,799 + +0.6 1,654,807 - -1.9 1,654,807 - -0.9 1,654,812 - -2.5 1,654,812 - +0.2 1,655,022 - ynfC b1585 0.35 +0.2 1,655,058 + ynfC b1585 0.40 -0.9 1,655,077 - ynfC b1585 0.43 +0.6 1,655,077 - ynfC b1585 0.43 +0.9 1,655,102 + ynfC b1585 0.47 -0.9 1,655,114 + ynfC b1585 0.48 +2.0 1,655,123 - ynfC b1585 0.50 -0.3 1,655,123 - ynfC b1585 0.50 +0.5 1,655,147 - ynfC b1585 0.53 +1.3 1,655,175 - ynfC b1585 0.57 +0.2 1,655,199 - ynfC b1585 0.60 +1.5 1,655,208 + ynfC b1585 0.61 -0.3 1,655,216 - ynfC b1585 0.63 -0.0 1,655,394 + ynfC b1585 0.88 +0.6 1,655,402 - +0.2 1,655,402 - ynfC b1585 0.89 -0.1 1,655,413 - +0.6 1,655,428 + -0.0 1,655,500 - -0.4 1,655,500 - -0.9 1,655,626 + b1586 b1586 0.12 +0.2 1,655,634 - b1586 b1586 0.15 +0.3 1,655,643 + b1586 b1586 0.18 +1.0 1,655,645 + b1586 b1586 0.18 +0.5 1,655,655 + b1586 b1586 0.22 -0.0 1,655,655 + b1586 b1586 0.22 +0.9 1,655,786 - b1586 b1586 0.64 +0.6 1,655,786 - b1586 b1586 0.64 +0.9 1,655,841 + b1586 b1586 0.82 +0.1 1,655,858 + b1586 b1586 0.88 +0.2 1,655,955 - +0.2 1,656,025 - +0.4 1,656,291 - -0.3 1,656,291 - +0.1 1,656,418 - ynfE b1587 0.13 +0.5 1,656,569 - ynfE b1587 0.20 +0.7 1,656,673 - ynfE b1587 0.24 -0.2 1,656,712 - ynfE b1587 0.26 +0.8 1,656,712 - ynfE b1587 0.26 -0.3 1,656,800 + ynfE b1587 0.29 +0.4 1,656,800 + ynfE b1587 0.29 +0.2
Or see this region's nucleotide sequence