Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydbK and ydbJ are separated by 273 nucleotides ydbJ and hslJ overlap by 4 nucleotides hslJ and ldhA are separated by 110 nucleotides
b1378: ydbK - fused predicted pyruvate-flavodoxin oxidoreductase: conserved protein/conserved protein/FeS binding protein (NCBI), at 1,435,284 to 1,438,808
ydbK
b4529: ydbJ - hypothetical protein (NCBI), at 1,439,082 to 1,439,348
ydbJ
b1379: hslJ - heat-inducible protein (NCBI), at 1,439,345 to 1,439,767
hslJ
b1380: ldhA - D-lactate dehydrogenase (NCBI), at 1,439,878 to 1,440,867
ldhA
Position (kb)
1439
1440 Strain fitness (log2 ratio)
-1
0
1 at 1438.460 kb on + strand at 1438.501 kb on + strand at 1438.540 kb on - strand at 1438.709 kb on - strand at 1438.815 kb on + strand at 1438.823 kb on - strand at 1438.926 kb on - strand at 1438.926 kb on - strand at 1438.997 kb on + strand at 1438.998 kb on - strand at 1439.084 kb on - strand at 1439.084 kb on - strand at 1439.137 kb on - strand, within ydbJ at 1439.365 kb on + strand at 1439.401 kb on - strand, within hslJ at 1439.561 kb on - strand, within hslJ at 1439.561 kb on - strand, within hslJ at 1439.591 kb on + strand, within hslJ at 1439.591 kb on + strand, within hslJ at 1439.654 kb on + strand, within hslJ at 1439.836 kb on - strand at 1439.836 kb on - strand at 1439.865 kb on - strand at 1439.929 kb on + strand at 1439.995 kb on - strand, within ldhA at 1440.256 kb on + strand, within ldhA at 1440.401 kb on - strand, within ldhA at 1440.401 kb on - strand, within ldhA
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 1,438,460 + -0.1 1,438,501 + +0.4 1,438,540 - +0.1 1,438,709 - -0.2 1,438,815 + +0.4 1,438,823 - +0.1 1,438,926 - +0.5 1,438,926 - +0.8 1,438,997 + +0.4 1,438,998 - +0.7 1,439,084 - +0.2 1,439,084 - +0.5 1,439,137 - ydbJ b4529 0.21 +0.4 1,439,365 + +0.1 1,439,401 - hslJ b1379 0.13 +0.0 1,439,561 - hslJ b1379 0.51 +0.7 1,439,561 - hslJ b1379 0.51 +0.5 1,439,591 + hslJ b1379 0.58 +0.8 1,439,591 + hslJ b1379 0.58 +1.4 1,439,654 + hslJ b1379 0.73 -0.1 1,439,836 - -0.8 1,439,836 - +0.1 1,439,865 - +0.4 1,439,929 + -0.1 1,439,995 - ldhA b1380 0.12 +0.1 1,440,256 + ldhA b1380 0.38 -1.0 1,440,401 - ldhA b1380 0.53 -1.0 1,440,401 - ldhA b1380 0.53 +0.1
Or see this region's nucleotide sequence