Experiment: 48 hr rep2_Tiberius_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybfK and kdpE are separated by 215 nucleotides kdpE and kdpD overlap by 4 nucleotides kdpD and kdpC overlap by 8 nucleotides kdpC and kdpB are separated by 8 nucleotides
b4590: ybfK - hypothetical protein (RefSeq), at 719,806 to 720,063
ybfK
b0694: kdpE - DNA-binding response regulator in two-component regulatory system with KdpD (NCBI), at 720,279 to 720,956
kdpE
b0695: kdpD - fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein (NCBI), at 720,953 to 723,637
kdpD
b0696: kdpC - potassium-transporting ATPase subunit C (NCBI), at 723,630 to 724,202
kdpC
b0697: kdpB - potassium-transporting ATPase subunit B (NCBI), at 724,211 to 726,259
kdpB
Position (kb) 720
721
722
723
724 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 720.247 kb on - strand at 720.247 kb on - strand at 720.298 kb on - strand at 720.305 kb on - strand at 720.321 kb on - strand at 720.323 kb on + strand at 720.330 kb on - strand at 720.330 kb on - strand at 720.331 kb on - strand at 720.332 kb on - strand at 720.332 kb on - strand at 720.336 kb on - strand at 720.358 kb on - strand, within kdpE at 720.391 kb on - strand, within kdpE at 720.403 kb on - strand, within kdpE at 720.420 kb on - strand, within kdpE at 720.441 kb on + strand, within kdpE at 720.441 kb on + strand, within kdpE at 720.449 kb on - strand, within kdpE at 720.481 kb on - strand, within kdpE at 720.512 kb on + strand, within kdpE at 720.512 kb on + strand, within kdpE at 720.627 kb on + strand, within kdpE at 720.678 kb on + strand, within kdpE at 720.678 kb on + strand, within kdpE at 720.745 kb on - strand, within kdpE at 720.785 kb on - strand, within kdpE at 721.045 kb on + strand at 721.045 kb on + strand at 721.053 kb on - strand at 721.061 kb on + strand at 721.061 kb on + strand at 721.301 kb on + strand, within kdpD at 721.301 kb on + strand, within kdpD at 721.425 kb on - strand, within kdpD at 721.425 kb on - strand, within kdpD at 721.484 kb on - strand, within kdpD at 721.489 kb on + strand, within kdpD at 721.504 kb on + strand at 721.702 kb on - strand, within kdpD at 721.714 kb on + strand, within kdpD at 721.815 kb on - strand, within kdpD at 721.815 kb on - strand, within kdpD at 721.902 kb on + strand, within kdpD at 721.910 kb on - strand, within kdpD at 722.048 kb on + strand at 722.058 kb on + strand, within kdpD at 722.076 kb on - strand, within kdpD at 722.142 kb on - strand, within kdpD at 722.301 kb on - strand, within kdpD at 722.315 kb on - strand, within kdpD at 722.351 kb on + strand, within kdpD at 722.355 kb on - strand, within kdpD at 722.459 kb on - strand, within kdpD at 722.533 kb on - strand, within kdpD at 722.630 kb on + strand, within kdpD at 722.673 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.769 kb on + strand, within kdpD at 722.769 kb on + strand, within kdpD at 722.812 kb on - strand, within kdpD at 722.812 kb on - strand, within kdpD at 723.084 kb on + strand, within kdpD at 723.162 kb on - strand, within kdpD at 723.184 kb on - strand, within kdpD at 723.184 kb on - strand, within kdpD at 723.323 kb on - strand, within kdpD at 723.323 kb on - strand, within kdpD at 723.351 kb on - strand, within kdpD at 723.596 kb on + strand at 723.658 kb on - strand at 723.961 kb on - strand, within kdpC at 723.961 kb on - strand, within kdpC at 724.046 kb on - strand, within kdpC at 724.060 kb on + strand, within kdpC at 724.198 kb on + strand at 724.216 kb on - strand at 724.216 kb on - strand at 724.234 kb on + strand at 724.234 kb on + strand at 724.279 kb on + strand at 724.315 kb on - strand at 724.467 kb on - strand, within kdpB at 724.467 kb on - strand, within kdpB at 724.546 kb on - strand, within kdpB
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution remove 720,247 - -0.6 720,247 - -0.4 720,298 - -0.2 720,305 - +0.8 720,321 - +0.1 720,323 + +1.2 720,330 - +0.6 720,330 - -0.1 720,331 - -0.5 720,332 - +0.0 720,332 - +0.1 720,336 - -1.2 720,358 - kdpE b0694 0.12 +0.3 720,391 - kdpE b0694 0.17 +1.4 720,403 - kdpE b0694 0.18 +0.5 720,420 - kdpE b0694 0.21 +0.7 720,441 + kdpE b0694 0.24 +0.3 720,441 + kdpE b0694 0.24 +0.2 720,449 - kdpE b0694 0.25 -0.0 720,481 - kdpE b0694 0.30 +0.6 720,512 + kdpE b0694 0.34 +0.1 720,512 + kdpE b0694 0.34 -0.4 720,627 + kdpE b0694 0.51 -0.9 720,678 + kdpE b0694 0.59 -0.1 720,678 + kdpE b0694 0.59 +0.5 720,745 - kdpE b0694 0.69 -0.3 720,785 - kdpE b0694 0.75 +0.1 721,045 + +0.0 721,045 + +0.2 721,053 - -0.6 721,061 + -0.1 721,061 + -0.3 721,301 + kdpD b0695 0.13 -0.1 721,301 + kdpD b0695 0.13 +0.6 721,425 - kdpD b0695 0.18 -0.5 721,425 - kdpD b0695 0.18 +0.3 721,484 - kdpD b0695 0.20 -0.9 721,489 + kdpD b0695 0.20 +0.6 721,504 + -0.8 721,702 - kdpD b0695 0.28 +0.1 721,714 + kdpD b0695 0.28 +1.3 721,815 - kdpD b0695 0.32 -0.2 721,815 - kdpD b0695 0.32 -0.8 721,902 + kdpD b0695 0.35 +0.2 721,910 - kdpD b0695 0.36 -0.3 722,048 + +3.6 722,058 + kdpD b0695 0.41 +0.3 722,076 - kdpD b0695 0.42 -0.7 722,142 - kdpD b0695 0.44 -0.8 722,301 - kdpD b0695 0.50 -1.5 722,315 - kdpD b0695 0.51 -1.0 722,351 + kdpD b0695 0.52 -0.4 722,355 - kdpD b0695 0.52 -0.3 722,459 - kdpD b0695 0.56 -1.3 722,533 - kdpD b0695 0.59 -0.8 722,630 + kdpD b0695 0.62 -0.4 722,673 + kdpD b0695 0.64 -0.8 722,762 + kdpD b0695 0.67 -0.7 722,762 + kdpD b0695 0.67 -0.2 722,762 + kdpD b0695 0.67 -0.4 722,769 + kdpD b0695 0.68 -0.9 722,769 + kdpD b0695 0.68 -1.0 722,812 - kdpD b0695 0.69 -0.2 722,812 - kdpD b0695 0.69 -1.1 723,084 + kdpD b0695 0.79 -0.0 723,162 - kdpD b0695 0.82 -0.9 723,184 - kdpD b0695 0.83 +1.0 723,184 - kdpD b0695 0.83 -1.2 723,323 - kdpD b0695 0.88 -1.1 723,323 - kdpD b0695 0.88 -2.4 723,351 - kdpD b0695 0.89 +0.1 723,596 + +0.1 723,658 - +0.2 723,961 - kdpC b0696 0.58 +0.1 723,961 - kdpC b0696 0.58 +0.2 724,046 - kdpC b0696 0.73 -0.1 724,060 + kdpC b0696 0.75 -0.5 724,198 + +0.9 724,216 - -1.2 724,216 - +0.1 724,234 + +0.5 724,234 + +0.5 724,279 + -0.6 724,315 - -0.4 724,467 - kdpB b0697 0.12 +0.1 724,467 - kdpB b0697 0.12 -0.8 724,546 - kdpB b0697 0.16 +0.7
Or see this region's nucleotide sequence