Strain Fitness in Escherichia coli BW25113 around b0002

Experiment: 48 hr rep2_Tiberius_no_dilution

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrL and thrA are separated by 81 nucleotidesthrA and thrB are separated by 1 nucleotidesthrB and thrC are separated by 0 nucleotides b0001: thrL - thr operon leader peptide (NCBI), at 190 to 255 thrL b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799 thrA b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733 thrB b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020 thrC Position (kb) 0 1 2 3Strain fitness (log2 ratio) -1 0 1 2 3 4at 0.070 kb on + strandat 0.070 kb on + strandat 0.078 kb on - strandat 0.078 kb on - strandat 0.161 kb on - strandat 0.185 kb on - strandat 0.185 kb on - strandat 0.208 kb on + strand, within thrLat 0.217 kb on - strand, within thrLat 0.257 kb on - strandat 0.257 kb on - strandat 0.440 kb on - strandat 0.440 kb on - strandat 0.454 kb on - strandat 0.518 kb on - strandat 0.518 kb on - strandat 0.528 kb on + strandat 0.592 kb on + strand, within thrAat 0.592 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.675 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.767 kb on + strand, within thrAat 0.775 kb on - strand, within thrAat 0.957 kb on + strand, within thrAat 0.957 kb on + strand, within thrAat 1.046 kb on + strand, within thrAat 1.136 kb on - strand, within thrAat 1.142 kb on - strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrAat 1.259 kb on - strand, within thrAat 1.259 kb on - strand, within thrAat 1.262 kb on + strand, within thrAat 1.270 kb on - strand, within thrAat 1.270 kb on - strand, within thrAat 1.280 kb on - strand, within thrAat 1.489 kb on + strand, within thrAat 1.599 kb on - strand, within thrAat 1.606 kb on + strand, within thrAat 1.627 kb on + strand, within thrAat 1.633 kb on - strand, within thrAat 1.633 kb on - strand, within thrAat 1.636 kb on + strand, within thrAat 1.636 kb on + strand, within thrAat 1.637 kb on + strand, within thrAat 1.711 kb on - strand, within thrAat 1.721 kb on - strand, within thrAat 1.767 kb on - strand, within thrAat 1.793 kb on - strand, within thrAat 1.868 kb on + strand, within thrAat 1.868 kb on + strand, within thrAat 1.871 kb on + strand, within thrAat 1.876 kb on - strand, within thrAat 1.881 kb on - strand, within thrAat 1.881 kb on - strand, within thrAat 1.929 kb on + strand, within thrAat 1.929 kb on + strand, within thrAat 1.937 kb on - strand, within thrAat 1.937 kb on - strand, within thrAat 2.076 kb on + strand, within thrAat 2.104 kb on + strand, within thrAat 2.104 kb on + strand, within thrAat 2.266 kb on - strand, within thrAat 2.266 kb on - strand, within thrAat 2.292 kb on - strand, within thrAat 2.294 kb on + strand, within thrAat 2.370 kb on - strand, within thrAat 2.370 kb on - strand, within thrAat 2.449 kb on - strand, within thrAat 2.590 kb on - strandat 2.675 kb on + strandat 2.682 kb on + strandat 2.682 kb on + strandat 2.684 kb on + strandat 2.717 kb on + strandat 2.744 kb on - strandat 2.787 kb on - strandat 2.787 kb on - strandat 2.829 kb on + strandat 2.847 kb on + strandat 2.847 kb on + strandat 2.906 kb on - strand, within thrBat 2.934 kb on + strand, within thrBat 2.963 kb on - strand, within thrBat 3.020 kb on - strand, within thrBat 3.023 kb on + strand, within thrBat 3.131 kb on + strand, within thrBat 3.144 kb on - strand, within thrBat 3.186 kb on + strand, within thrBat 3.246 kb on + strand, within thrBat 3.248 kb on + strand, within thrBat 3.256 kb on + strand, within thrBat 3.293 kb on + strand, within thrBat 3.301 kb on - strand, within thrBat 3.318 kb on - strand, within thrBat 3.411 kb on + strand, within thrBat 3.444 kb on - strand, within thrBat 3.444 kb on - strand, within thrBat 3.504 kb on - strand, within thrBat 3.504 kb on - strand, within thrBat 3.509 kb on - strand, within thrBat 3.511 kb on + strand, within thrBat 3.607 kb on + strand, within thrBat 3.634 kb on - strand, within thrBat 3.638 kb on + strand, within thrBat 3.638 kb on + strand, within thrBat 3.650 kb on + strandat 3.769 kb on + strandat 3.769 kb on + strandat 3.792 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 48 hr rep2_Tiberius_no_dilution
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70 + -0.4
70 + +0.2
78 - +0.4
78 - +1.3
161 - +0.6
185 - +2.1
185 - +2.6
208 + thrL b0001 0.27 -0.1
217 - thrL b0001 0.41 +0.6
257 - +0.3
257 - +0.8
440 - +0.5
440 - +1.1
454 - +0.5
518 - +0.6
518 - +0.8
528 + -0.5
592 + thrA b0002 0.10 +0.7
592 + thrA b0002 0.10 -0.6
666 + thrA b0002 0.13 +1.1
666 + thrA b0002 0.13 +1.0
675 - thrA b0002 0.14 +0.5
754 - thrA b0002 0.17 +0.4
754 - thrA b0002 0.17 +0.3
767 + thrA b0002 0.17 +0.5
775 - thrA b0002 0.18 +1.0
957 + thrA b0002 0.25 +1.3
957 + thrA b0002 0.25 +0.3
1,046 + thrA b0002 0.29 +1.3
1,136 - thrA b0002 0.32 +0.4
1,142 - thrA b0002 0.33 +0.8
1,161 + thrA b0002 0.33 +0.0
1,161 + thrA b0002 0.33 +0.6
1,161 + thrA b0002 0.33 +0.6
1,259 - thrA b0002 0.37 +0.2
1,259 - thrA b0002 0.37 +0.2
1,262 + thrA b0002 0.38 +0.6
1,270 - thrA b0002 0.38 +1.6
1,270 - thrA b0002 0.38 +0.8
1,280 - thrA b0002 0.38 +0.3
1,489 + thrA b0002 0.47 +0.9
1,599 - thrA b0002 0.51 +1.4
1,606 + thrA b0002 0.52 +0.7
1,627 + thrA b0002 0.52 -0.1
1,633 - thrA b0002 0.53 +0.8
1,633 - thrA b0002 0.53 +0.4
1,636 + thrA b0002 0.53 +1.4
1,636 + thrA b0002 0.53 +0.8
1,637 + thrA b0002 0.53 +0.5
1,711 - thrA b0002 0.56 +0.4
1,721 - thrA b0002 0.56 +0.6
1,767 - thrA b0002 0.58 +0.2
1,793 - thrA b0002 0.59 +0.5
1,868 + thrA b0002 0.62 +0.8
1,868 + thrA b0002 0.62 +1.0
1,871 + thrA b0002 0.62 +0.8
1,876 - thrA b0002 0.62 +0.7
1,881 - thrA b0002 0.63 +0.0
1,881 - thrA b0002 0.63 +1.3
1,929 + thrA b0002 0.65 +0.4
1,929 + thrA b0002 0.65 +0.6
1,937 - thrA b0002 0.65 +0.7
1,937 - thrA b0002 0.65 +4.4
2,076 + thrA b0002 0.71 +0.1
2,104 + thrA b0002 0.72 +0.7
2,104 + thrA b0002 0.72 +0.7
2,266 - thrA b0002 0.78 +1.1
2,266 - thrA b0002 0.78 +0.2
2,292 - thrA b0002 0.79 +0.9
2,294 + thrA b0002 0.79 +0.4
2,370 - thrA b0002 0.83 +1.5
2,370 - thrA b0002 0.83 -0.2
2,449 - thrA b0002 0.86 -0.0
2,590 - +0.4
2,675 + +0.6
2,682 + +1.1
2,682 + +1.4
2,684 + +0.3
2,717 + +0.8
2,744 - +0.8
2,787 - +0.5
2,787 - +1.8
2,829 + +0.9
2,847 + +1.5
2,847 + +0.8
2,906 - thrB b0003 0.11 +0.4
2,934 + thrB b0003 0.14 +0.9
2,963 - thrB b0003 0.17 +0.9
3,020 - thrB b0003 0.23 +1.2
3,023 + thrB b0003 0.24 +0.9
3,131 + thrB b0003 0.35 +1.0
3,144 - thrB b0003 0.37 +0.7
3,186 + thrB b0003 0.41 +0.7
3,246 + thrB b0003 0.48 +1.1
3,248 + thrB b0003 0.48 +0.9
3,256 + thrB b0003 0.49 +1.0
3,293 + thrB b0003 0.53 +0.3
3,301 - thrB b0003 0.54 +0.8
3,318 - thrB b0003 0.55 +0.7
3,411 + thrB b0003 0.65 +2.0
3,444 - thrB b0003 0.69 +1.8
3,444 - thrB b0003 0.69 -0.0
3,504 - thrB b0003 0.75 +0.6
3,504 - thrB b0003 0.75 +0.9
3,509 - thrB b0003 0.76 +0.9
3,511 + thrB b0003 0.76 +0.9
3,607 + thrB b0003 0.86 +1.1
3,634 - thrB b0003 0.89 +0.7
3,638 + thrB b0003 0.90 +0.9
3,638 + thrB b0003 0.90 +0.3
3,650 + +0.7
3,769 + +1.1
3,769 + +0.5
3,792 - +0.6

Or see this region's nucleotide sequence