Strain Fitness in Escherichia coli BW25113 around b3367

Experiment: 48 hr rep3_HD100_no_dilution

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntnirB and nirD overlap by 4 nucleotidesnirD and nirC are separated by 125 nucleotidesnirC and cysG are separated by 18 nucleotides b3365: nirB - nitrite reductase, large subunit, NAD(P)H-binding (NCBI), at 3,492,033 to 3,494,576 nirB b3366: nirD - nitrite reductase small subunit (NCBI), at 3,494,573 to 3,494,899 nirD b3367: nirC - nitrite transporter (NCBI), at 3,495,025 to 3,495,831 nirC b3368: cysG - fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI), at 3,495,850 to 3,497,223 cysG Position (kb) 3495 3496Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3494.041 kb on - strand, within nirBat 3494.107 kb on - strand, within nirBat 3494.107 kb on - strand, within nirBat 3494.247 kb on + strand, within nirBat 3494.347 kb on + strandat 3494.395 kb on + strandat 3494.434 kb on + strandat 3494.434 kb on + strandat 3494.452 kb on - strandat 3494.459 kb on + strandat 3494.488 kb on + strandat 3494.499 kb on - strandat 3494.499 kb on - strandat 3494.507 kb on - strandat 3494.507 kb on - strandat 3494.507 kb on - strandat 3494.540 kb on - strandat 3494.548 kb on + strandat 3494.564 kb on - strandat 3494.577 kb on - strandat 3494.577 kb on - strandat 3494.646 kb on - strand, within nirDat 3494.742 kb on - strand, within nirDat 3494.760 kb on + strand, within nirDat 3494.765 kb on + strand, within nirDat 3494.765 kb on + strand, within nirDat 3494.765 kb on + strand, within nirDat 3494.779 kb on - strand, within nirDat 3494.888 kb on - strandat 3494.888 kb on - strandat 3494.939 kb on + strandat 3494.939 kb on + strandat 3494.947 kb on - strandat 3494.947 kb on - strandat 3495.048 kb on + strandat 3495.056 kb on - strandat 3495.062 kb on - strandat 3495.071 kb on + strandat 3495.079 kb on - strandat 3495.113 kb on + strand, within nirCat 3495.113 kb on + strand, within nirCat 3495.123 kb on + strand, within nirCat 3495.124 kb on - strand, within nirCat 3495.124 kb on - strand, within nirCat 3495.129 kb on + strand, within nirCat 3495.129 kb on + strand, within nirCat 3495.131 kb on + strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.148 kb on - strand, within nirCat 3495.182 kb on + strand, within nirCat 3495.195 kb on - strand, within nirCat 3495.197 kb on + strand, within nirCat 3495.197 kb on + strand, within nirCat 3495.251 kb on + strand, within nirCat 3495.251 kb on + strand, within nirCat 3495.251 kb on + strand, within nirCat 3495.259 kb on - strand, within nirCat 3495.271 kb on - strand, within nirCat 3495.308 kb on + strand, within nirCat 3495.308 kb on + strand, within nirCat 3495.311 kb on - strand, within nirCat 3495.311 kb on - strand, within nirCat 3495.353 kb on - strand, within nirCat 3495.358 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.388 kb on + strand, within nirCat 3495.396 kb on - strand, within nirCat 3495.491 kb on + strand, within nirCat 3495.535 kb on + strand, within nirCat 3495.579 kb on - strand, within nirCat 3495.609 kb on + strand, within nirCat 3495.623 kb on - strand, within nirCat 3495.623 kb on - strand, within nirCat 3495.626 kb on + strand, within nirCat 3495.626 kb on + strand, within nirCat 3495.645 kb on + strand, within nirCat 3495.645 kb on + strand, within nirCat 3495.653 kb on + strand, within nirCat 3495.654 kb on - strand, within nirCat 3495.678 kb on - strand, within nirCat 3495.678 kb on - strand, within nirCat 3495.702 kb on + strand, within nirCat 3495.705 kb on - strand, within nirCat 3495.710 kb on - strand, within nirCat 3495.715 kb on - strand, within nirCat 3495.717 kb on + strand, within nirCat 3495.744 kb on - strand, within nirCat 3495.832 kb on - strandat 3495.893 kb on + strandat 3495.932 kb on - strandat 3495.936 kb on + strandat 3495.983 kb on + strandat 3495.993 kb on + strand, within cysGat 3496.019 kb on + strand, within cysGat 3496.019 kb on + strand, within cysGat 3496.019 kb on + strand, within cysGat 3496.026 kb on - strand, within cysGat 3496.065 kb on - strand, within cysGat 3496.070 kb on - strand, within cysGat 3496.100 kb on + strand, within cysGat 3496.100 kb on + strand, within cysGat 3496.119 kb on - strand, within cysGat 3496.119 kb on - strand, within cysGat 3496.237 kb on - strand, within cysGat 3496.300 kb on - strand, within cysGat 3496.300 kb on - strand, within cysGat 3496.345 kb on + strand, within cysGat 3496.351 kb on + strand, within cysGat 3496.371 kb on - strand, within cysGat 3496.417 kb on - strand, within cysGat 3496.422 kb on - strand, within cysGat 3496.456 kb on - strand, within cysGat 3496.502 kb on - strand, within cysGat 3496.504 kb on + strand, within cysGat 3496.504 kb on + strand, within cysGat 3496.514 kb on + strand, within cysGat 3496.514 kb on + strand, within cysGat 3496.514 kb on + strand, within cysGat 3496.519 kb on - strand, within cysGat 3496.529 kb on + strand, within cysGat 3496.529 kb on + strand, within cysGat 3496.539 kb on + strand, within cysGat 3496.539 kb on + strand, within cysGat 3496.582 kb on - strand, within cysGat 3496.582 kb on - strand, within cysGat 3496.582 kb on - strand, within cysGat 3496.585 kb on + strand, within cysGat 3496.585 kb on + strand, within cysGat 3496.585 kb on + strand, within cysGat 3496.585 kb on - strand, within cysGat 3496.617 kb on - strand, within cysGat 3496.622 kb on - strand, within cysGat 3496.634 kb on + strand, within cysGat 3496.666 kb on + strand, within cysGat 3496.666 kb on + strand, within cysGat 3496.675 kb on + strand, within cysGat 3496.675 kb on + strand, within cysGat 3496.679 kb on - strand, within cysGat 3496.679 kb on - strand, within cysGat 3496.682 kb on - strand, within cysGat 3496.697 kb on + strand, within cysGat 3496.697 kb on + strand, within cysGat 3496.702 kb on + strand, within cysGat 3496.702 kb on + strand, within cysGat 3496.708 kb on - strand, within cysGat 3496.786 kb on + strand, within cysGat 3496.794 kb on - strand, within cysGat 3496.797 kb on + strand, within cysGat 3496.800 kb on + strand, within cysGat 3496.800 kb on + strand, within cysGat 3496.800 kb on + strand, within cysGat 3496.823 kb on + strand, within cysGat 3496.823 kb on + strand, within cysGat 3496.825 kb on + strand, within cysG

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 48 hr rep3_HD100_no_dilution
remove
3,494,041 - nirB b3365 0.79 +1.7
3,494,107 - nirB b3365 0.82 +0.6
3,494,107 - nirB b3365 0.82 -0.4
3,494,247 + nirB b3365 0.87 +0.7
3,494,347 + +0.1
3,494,395 + +0.0
3,494,434 + +1.2
3,494,434 + -1.1
3,494,452 - +0.2
3,494,459 + -0.1
3,494,488 + +0.4
3,494,499 - -0.5
3,494,499 - -0.1
3,494,507 - +0.2
3,494,507 - +0.5
3,494,507 - -0.4
3,494,540 - +0.2
3,494,548 + -0.2
3,494,564 - -0.0
3,494,577 - -0.2
3,494,577 - +0.6
3,494,646 - nirD b3366 0.22 -0.0
3,494,742 - nirD b3366 0.52 +0.2
3,494,760 + nirD b3366 0.57 -0.3
3,494,765 + nirD b3366 0.59 -0.1
3,494,765 + nirD b3366 0.59 +0.4
3,494,765 + nirD b3366 0.59 +1.3
3,494,779 - nirD b3366 0.63 +0.5
3,494,888 - -0.4
3,494,888 - +0.0
3,494,939 + -0.2
3,494,939 + -0.2
3,494,947 - +0.2
3,494,947 - +0.0
3,495,048 + +0.0
3,495,056 - +0.3
3,495,062 - +0.4
3,495,071 + -0.1
3,495,079 - +0.0
3,495,113 + nirC b3367 0.11 -0.7
3,495,113 + nirC b3367 0.11 +1.3
3,495,123 + nirC b3367 0.12 +0.4
3,495,124 - nirC b3367 0.12 -0.1
3,495,124 - nirC b3367 0.12 -0.8
3,495,129 + nirC b3367 0.13 +0.2
3,495,129 + nirC b3367 0.13 +1.7
3,495,131 + nirC b3367 0.13 +0.5
3,495,148 - nirC b3367 0.15 -0.9
3,495,148 - nirC b3367 0.15 +0.5
3,495,148 - nirC b3367 0.15 +0.1
3,495,148 - nirC b3367 0.15 -0.0
3,495,182 + nirC b3367 0.19 +0.1
3,495,195 - nirC b3367 0.21 +0.4
3,495,197 + nirC b3367 0.21 -0.1
3,495,197 + nirC b3367 0.21 -0.2
3,495,251 + nirC b3367 0.28 -0.4
3,495,251 + nirC b3367 0.28 -0.5
3,495,251 + nirC b3367 0.28 -0.4
3,495,259 - nirC b3367 0.29 -0.3
3,495,271 - nirC b3367 0.30 -0.3
3,495,308 + nirC b3367 0.35 +0.6
3,495,308 + nirC b3367 0.35 -0.8
3,495,311 - nirC b3367 0.35 -0.3
3,495,311 - nirC b3367 0.35 +0.5
3,495,353 - nirC b3367 0.41 -0.4
3,495,358 + nirC b3367 0.41 +0.2
3,495,388 + nirC b3367 0.45 -0.4
3,495,388 + nirC b3367 0.45 +0.1
3,495,388 + nirC b3367 0.45 +0.1
3,495,388 + nirC b3367 0.45 -0.5
3,495,388 + nirC b3367 0.45 -0.0
3,495,396 - nirC b3367 0.46 +0.2
3,495,491 + nirC b3367 0.58 +0.4
3,495,535 + nirC b3367 0.63 +0.1
3,495,579 - nirC b3367 0.69 -0.5
3,495,609 + nirC b3367 0.72 -3.3
3,495,623 - nirC b3367 0.74 +1.1
3,495,623 - nirC b3367 0.74 -0.1
3,495,626 + nirC b3367 0.74 -0.4
3,495,626 + nirC b3367 0.74 -0.1
3,495,645 + nirC b3367 0.77 +0.3
3,495,645 + nirC b3367 0.77 +0.5
3,495,653 + nirC b3367 0.78 -0.5
3,495,654 - nirC b3367 0.78 +0.7
3,495,678 - nirC b3367 0.81 -1.3
3,495,678 - nirC b3367 0.81 +1.6
3,495,702 + nirC b3367 0.84 -0.2
3,495,705 - nirC b3367 0.84 -0.0
3,495,710 - nirC b3367 0.85 -0.2
3,495,715 - nirC b3367 0.86 +0.5
3,495,717 + nirC b3367 0.86 -0.2
3,495,744 - nirC b3367 0.89 -0.3
3,495,832 - +0.3
3,495,893 + +0.1
3,495,932 - +1.0
3,495,936 + -0.1
3,495,983 + +0.2
3,495,993 + cysG b3368 0.10 +1.3
3,496,019 + cysG b3368 0.12 +0.3
3,496,019 + cysG b3368 0.12 -0.2
3,496,019 + cysG b3368 0.12 -0.2
3,496,026 - cysG b3368 0.13 +0.3
3,496,065 - cysG b3368 0.16 +0.2
3,496,070 - cysG b3368 0.16 +0.6
3,496,100 + cysG b3368 0.18 -0.4
3,496,100 + cysG b3368 0.18 +0.2
3,496,119 - cysG b3368 0.20 -0.2
3,496,119 - cysG b3368 0.20 +0.2
3,496,237 - cysG b3368 0.28 +0.6
3,496,300 - cysG b3368 0.33 -0.2
3,496,300 - cysG b3368 0.33 -0.2
3,496,345 + cysG b3368 0.36 +0.3
3,496,351 + cysG b3368 0.36 -0.2
3,496,371 - cysG b3368 0.38 +0.5
3,496,417 - cysG b3368 0.41 -0.2
3,496,422 - cysG b3368 0.42 +0.1
3,496,456 - cysG b3368 0.44 +0.8
3,496,502 - cysG b3368 0.47 +0.4
3,496,504 + cysG b3368 0.48 +0.7
3,496,504 + cysG b3368 0.48 +0.2
3,496,514 + cysG b3368 0.48 +0.6
3,496,514 + cysG b3368 0.48 -0.3
3,496,514 + cysG b3368 0.48 +0.6
3,496,519 - cysG b3368 0.49 +0.5
3,496,529 + cysG b3368 0.49 -0.7
3,496,529 + cysG b3368 0.49 -1.1
3,496,539 + cysG b3368 0.50 +1.2
3,496,539 + cysG b3368 0.50 +0.6
3,496,582 - cysG b3368 0.53 +1.2
3,496,582 - cysG b3368 0.53 +0.6
3,496,582 - cysG b3368 0.53 -0.2
3,496,585 + cysG b3368 0.53 +0.2
3,496,585 + cysG b3368 0.53 +0.6
3,496,585 + cysG b3368 0.53 -0.4
3,496,585 - cysG b3368 0.53 +1.5
3,496,617 - cysG b3368 0.56 +0.6
3,496,622 - cysG b3368 0.56 +0.8
3,496,634 + cysG b3368 0.57 +0.1
3,496,666 + cysG b3368 0.59 +0.6
3,496,666 + cysG b3368 0.59 -0.5
3,496,675 + cysG b3368 0.60 -0.7
3,496,675 + cysG b3368 0.60 -0.7
3,496,679 - cysG b3368 0.60 +1.0
3,496,679 - cysG b3368 0.60 +0.2
3,496,682 - cysG b3368 0.61 -0.4
3,496,697 + cysG b3368 0.62 +0.5
3,496,697 + cysG b3368 0.62 -0.1
3,496,702 + cysG b3368 0.62 +0.2
3,496,702 + cysG b3368 0.62 +0.3
3,496,708 - cysG b3368 0.62 -1.5
3,496,786 + cysG b3368 0.68 +0.1
3,496,794 - cysG b3368 0.69 +2.4
3,496,797 + cysG b3368 0.69 +0.4
3,496,800 + cysG b3368 0.69 -0.3
3,496,800 + cysG b3368 0.69 +1.8
3,496,800 + cysG b3368 0.69 -0.8
3,496,823 + cysG b3368 0.71 -0.0
3,496,823 + cysG b3368 0.71 +0.4
3,496,825 + cysG b3368 0.71 -0.1

Or see this region's nucleotide sequence