Experiment: 48 hr rep2_HD100_10fold_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proS and yaeB are separated by 111 nucleotides yaeB and rcsF overlap by 4 nucleotides rcsF and metQ are separated by 117 nucleotides
b0194: proS - prolyl-tRNA synthetase (NCBI), at 217,057 to 218,775
proS
b0195: yaeB - hypothetical protein (NCBI), at 218,887 to 219,594
yaeB
b0196: rcsF - predicted outer membrane protein, signal (NCBI), at 219,591 to 219,995
rcsF
b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928
metQ
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1 at 218.821 kb on - strand at 218.871 kb on + strand at 218.910 kb on + strand at 218.910 kb on + strand at 218.965 kb on + strand, within yaeB at 218.974 kb on - strand, within yaeB at 219.027 kb on - strand, within yaeB at 219.071 kb on - strand, within yaeB at 219.200 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.261 kb on + strand, within yaeB at 219.312 kb on + strand, within yaeB at 219.446 kb on - strand, within yaeB at 219.465 kb on + strand, within yaeB at 219.466 kb on + strand, within yaeB at 219.489 kb on - strand, within yaeB at 219.513 kb on + strand, within yaeB at 219.548 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.643 kb on - strand, within rcsF at 219.678 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.756 kb on + strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.842 kb on - strand, within rcsF at 219.902 kb on + strand, within rcsF at 219.944 kb on - strand, within rcsF at 219.968 kb on + strand at 219.976 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.127 kb on - strand at 220.127 kb on - strand at 220.249 kb on - strand, within metQ at 220.321 kb on + strand, within metQ at 220.321 kb on + strand, within metQ at 220.323 kb on + strand, within metQ at 220.361 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.405 kb on + strand, within metQ at 220.405 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.504 kb on + strand, within metQ at 220.532 kb on + strand, within metQ at 220.543 kb on + strand, within metQ
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep2_HD100_10fold_dilution remove 218,821 - +0.4 218,871 + -0.5 218,910 + +0.1 218,910 + +0.1 218,965 + yaeB b0195 0.11 -0.1 218,974 - yaeB b0195 0.12 +0.2 219,027 - yaeB b0195 0.20 -1.6 219,071 - yaeB b0195 0.26 +0.1 219,200 + yaeB b0195 0.44 -0.0 219,240 + yaeB b0195 0.50 +0.8 219,240 + yaeB b0195 0.50 -0.1 219,261 + yaeB b0195 0.53 -0.6 219,312 + yaeB b0195 0.60 +1.1 219,446 - yaeB b0195 0.79 +0.2 219,465 + yaeB b0195 0.82 +0.6 219,466 + yaeB b0195 0.82 -1.0 219,489 - yaeB b0195 0.85 +0.1 219,513 + yaeB b0195 0.88 -0.3 219,548 - -0.2 219,598 + +0.2 219,598 + +0.1 219,643 - rcsF b0196 0.13 +0.3 219,678 + rcsF b0196 0.21 +0.5 219,680 + rcsF b0196 0.22 -0.0 219,680 + rcsF b0196 0.22 +0.3 219,680 + rcsF b0196 0.22 +0.1 219,720 - rcsF b0196 0.32 -0.3 219,720 - rcsF b0196 0.32 -0.2 219,756 + rcsF b0196 0.41 +0.5 219,764 - rcsF b0196 0.43 -0.2 219,764 - rcsF b0196 0.43 -0.3 219,764 - rcsF b0196 0.43 +0.1 219,842 - rcsF b0196 0.62 +0.6 219,902 + rcsF b0196 0.77 +0.3 219,944 - rcsF b0196 0.87 +0.6 219,968 + +0.5 219,976 - +0.2 220,041 - +0.4 220,041 - -0.5 220,041 - -0.3 220,127 - -0.1 220,127 - -0.5 220,249 - metQ b0197 0.17 -0.8 220,321 + metQ b0197 0.25 -1.3 220,321 + metQ b0197 0.25 -0.6 220,323 + metQ b0197 0.26 -0.4 220,361 - metQ b0197 0.30 -0.8 220,386 - metQ b0197 0.33 -2.6 220,386 - metQ b0197 0.33 -1.4 220,405 + metQ b0197 0.36 -0.6 220,405 + metQ b0197 0.36 -0.0 220,432 + metQ b0197 0.39 -0.5 220,432 + metQ b0197 0.39 +0.7 220,504 + metQ b0197 0.48 +0.0 220,532 + metQ b0197 0.51 +0.3 220,543 + metQ b0197 0.53 -0.8
Or see this region's nucleotide sequence