Strain Fitness in Escherichia coli BW25113 around b0243

Experiment: 48 hr NoPredatorControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntproB and proA are separated by 11 nucleotidesproA and thrW are separated by 114 nucleotidesthrW and ykfN are separated by 203 nucleotidesykfN and ykfI are separated by 115 nucleotidesykfI and yafW are separated by 20 nucleotides b0242: proB - gamma-glutamyl kinase (NCBI), at 259,612 to 260,715 proB b0243: proA - gamma-glutamyl phosphate reductase (NCBI), at 260,727 to 261,980 proA b0244: thrW - tRNA-Thr (NCBI), at 262,095 to 262,170 thrW b4626: ykfN - no description, at 262,374 to 262,436 ykfN b0245: ykfI - CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system (NCBI), at 262,552 to 262,893 ykfI b0246: yafW - CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system (NCBI), at 262,914 to 263,231 yafW Position (kb) 260 261 262Strain fitness (log2 ratio) -2 -1 0 1 2 3at 259.804 kb on + strand, within proBat 259.851 kb on - strand, within proBat 260.024 kb on - strand, within proBat 260.048 kb on - strand, within proBat 260.138 kb on + strand, within proBat 260.243 kb on - strand, within proBat 260.279 kb on + strand, within proBat 260.378 kb on - strand, within proBat 260.378 kb on - strand, within proBat 260.506 kb on + strand, within proBat 260.506 kb on + strand, within proBat 260.569 kb on - strand, within proBat 260.594 kb on + strand, within proBat 260.609 kb on + strandat 260.611 kb on + strandat 260.625 kb on - strandat 260.629 kb on - strandat 260.666 kb on + strandat 260.666 kb on + strandat 260.760 kb on + strandat 260.852 kb on + strandat 260.932 kb on + strand, within proAat 260.932 kb on + strand, within proAat 260.932 kb on - strand, within proAat 260.932 kb on - strand, within proAat 260.981 kb on - strand, within proAat 260.983 kb on - strand, within proAat 260.983 kb on - strand, within proAat 261.176 kb on + strand, within proAat 261.266 kb on - strandat 261.266 kb on - strand, within proAat 261.360 kb on - strand, within proAat 261.360 kb on - strand, within proAat 261.388 kb on + strand, within proAat 261.439 kb on + strand, within proAat 261.449 kb on + strand, within proAat 261.449 kb on + strand, within proAat 261.476 kb on + strand, within proAat 261.476 kb on + strand, within proAat 261.583 kb on - strand, within proAat 261.583 kb on - strand, within proAat 261.605 kb on + strand, within proAat 261.656 kb on - strand, within proAat 261.670 kb on - strand, within proAat 261.670 kb on - strand, within proAat 261.746 kb on + strand, within proAat 261.759 kb on - strand, within proAat 261.759 kb on - strand, within proAat 261.824 kb on - strand, within proAat 261.824 kb on - strand, within proAat 261.869 kb on - strandat 261.916 kb on - strandat 261.916 kb on - strandat 261.960 kb on - strandat 261.960 kb on - strandat 262.045 kb on + strandat 262.091 kb on - strandat 262.113 kb on + strand, within thrWat 262.270 kb on + strandat 262.310 kb on + strandat 262.310 kb on + strandat 262.319 kb on + strandat 262.371 kb on + strandat 262.479 kb on - strandat 262.546 kb on - strandat 262.548 kb on + strandat 262.565 kb on + strandat 262.644 kb on - strand, within ykfIat 262.717 kb on - strand, within ykfIat 262.719 kb on + strand, within ykfIat 262.727 kb on - strand, within ykfIat 262.727 kb on - strand, within ykfIat 262.727 kb on - strand, within ykfIat 262.730 kb on + strand, within ykfIat 262.730 kb on + strand, within ykfIat 262.738 kb on - strand, within ykfIat 262.760 kb on + strand, within ykfIat 262.786 kb on - strand, within ykfIat 262.909 kb on - strandat 262.909 kb on - strandat 262.917 kb on + strandat 262.917 kb on + strandat 262.917 kb on + strandat 262.935 kb on - strandat 262.943 kb on + strandat 262.943 kb on + strandat 262.980 kb on + strand, within yafWat 262.980 kb on + strand, within yafW

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Per-strain Table

Position Strand Gene LocusTag Fraction 48 hr NoPredatorControl
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259,804 + proB b0242 0.17 +0.1
259,851 - proB b0242 0.22 -0.3
260,024 - proB b0242 0.37 +0.7
260,048 - proB b0242 0.39 +0.2
260,138 + proB b0242 0.48 +0.5
260,243 - proB b0242 0.57 +0.5
260,279 + proB b0242 0.60 +0.6
260,378 - proB b0242 0.69 +0.3
260,378 - proB b0242 0.69 -1.4
260,506 + proB b0242 0.81 -0.1
260,506 + proB b0242 0.81 +0.2
260,569 - proB b0242 0.87 -1.0
260,594 + proB b0242 0.89 -0.8
260,609 + -0.5
260,611 + -0.0
260,625 - +0.3
260,629 - -0.2
260,666 + +0.2
260,666 + +0.7
260,760 + +0.6
260,852 + -1.3
260,932 + proA b0243 0.16 +1.1
260,932 + proA b0243 0.16 +0.6
260,932 - proA b0243 0.16 +0.4
260,932 - proA b0243 0.16 +0.8
260,981 - proA b0243 0.20 +0.4
260,983 - proA b0243 0.20 -0.3
260,983 - proA b0243 0.20 -0.2
261,176 + proA b0243 0.36 +0.1
261,266 - +0.6
261,266 - proA b0243 0.43 -0.3
261,360 - proA b0243 0.50 -0.2
261,360 - proA b0243 0.50 -1.6
261,388 + proA b0243 0.53 -0.1
261,439 + proA b0243 0.57 -0.8
261,449 + proA b0243 0.58 +0.4
261,449 + proA b0243 0.58 -0.0
261,476 + proA b0243 0.60 +0.6
261,476 + proA b0243 0.60 +0.6
261,583 - proA b0243 0.68 +0.4
261,583 - proA b0243 0.68 +0.3
261,605 + proA b0243 0.70 +3.2
261,656 - proA b0243 0.74 +0.6
261,670 - proA b0243 0.75 -1.2
261,670 - proA b0243 0.75 -1.8
261,746 + proA b0243 0.81 +1.5
261,759 - proA b0243 0.82 -0.7
261,759 - proA b0243 0.82 +0.2
261,824 - proA b0243 0.87 -0.7
261,824 - proA b0243 0.87 +0.2
261,869 - -0.8
261,916 - +0.1
261,916 - +0.3
261,960 - +0.4
261,960 - +1.0
262,045 + +0.9
262,091 - +0.4
262,113 + thrW b0244 0.24 +0.2
262,270 + -1.1
262,310 + +0.3
262,310 + +0.6
262,319 + -0.1
262,371 + +0.3
262,479 - +0.5
262,546 - +0.3
262,548 + +0.0
262,565 + -0.2
262,644 - ykfI b0245 0.27 +0.8
262,717 - ykfI b0245 0.48 -0.3
262,719 + ykfI b0245 0.49 -0.3
262,727 - ykfI b0245 0.51 +1.0
262,727 - ykfI b0245 0.51 -0.2
262,727 - ykfI b0245 0.51 +0.3
262,730 + ykfI b0245 0.52 +0.4
262,730 + ykfI b0245 0.52 -0.2
262,738 - ykfI b0245 0.54 +0.1
262,760 + ykfI b0245 0.61 +0.2
262,786 - ykfI b0245 0.68 +0.5
262,909 - +0.0
262,909 - -0.2
262,917 + -2.1
262,917 + +0.7
262,917 + -1.6
262,935 - +1.6
262,943 + -0.7
262,943 + +0.2
262,980 + yafW b0246 0.21 +0.0
262,980 + yafW b0246 0.21 +0.7

Or see this region's nucleotide sequence