Experiment: 48 hr rep1_HD100_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjF and ydjG are separated by 136 nucleotides ydjG and b1772 are separated by 9 nucleotides b1772 and ydjI are separated by 4 nucleotides
b1770: ydjF - predicted DNA-binding transcriptional regulator (NCBI), at 1,852,120 to 1,852,878
ydjF
b1771: ydjG - predicted oxidoreductase (NCBI), at 1,853,015 to 1,853,995
ydjG
b1772: b1772 - putative kinase (VIMSS), at 1,854,005 to 1,854,952
b1772
b1773: ydjI - predicted aldolase (NCBI), at 1,854,957 to 1,855,793
ydjI
Position (kb)
1853
1854 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1852.035 kb on + strand at 1852.163 kb on - strand at 1852.165 kb on - strand at 1852.165 kb on - strand at 1852.165 kb on - strand at 1852.167 kb on - strand at 1852.172 kb on - strand at 1852.224 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.264 kb on - strand, within ydjF at 1852.264 kb on - strand, within ydjF at 1852.388 kb on - strand, within ydjF at 1852.457 kb on - strand, within ydjF at 1852.527 kb on + strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.576 kb on - strand, within ydjF at 1852.581 kb on - strand, within ydjF at 1852.606 kb on + strand, within ydjF at 1852.611 kb on - strand, within ydjF at 1852.636 kb on - strand, within ydjF at 1852.853 kb on + strand at 1853.029 kb on - strand at 1853.029 kb on - strand at 1853.048 kb on - strand at 1853.102 kb on + strand at 1853.110 kb on - strand at 1853.170 kb on + strand, within ydjG at 1853.192 kb on - strand, within ydjG at 1853.368 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.448 kb on + strand, within ydjG at 1853.537 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.751 kb on + strand, within ydjG at 1853.763 kb on - strand, within ydjG at 1853.919 kb on - strand at 1853.937 kb on - strand at 1853.947 kb on - strand at 1854.143 kb on - strand, within b1772 at 1854.143 kb on - strand, within b1772 at 1854.178 kb on - strand, within b1772 at 1854.178 kb on - strand, within b1772 at 1854.223 kb on - strand, within b1772 at 1854.246 kb on - strand, within b1772 at 1854.321 kb on - strand, within b1772 at 1854.389 kb on + strand, within b1772 at 1854.397 kb on - strand, within b1772 at 1854.425 kb on - strand, within b1772 at 1854.425 kb on - strand, within b1772 at 1854.461 kb on - strand, within b1772 at 1854.461 kb on - strand, within b1772 at 1854.550 kb on - strand, within b1772 at 1854.644 kb on - strand, within b1772 at 1854.644 kb on - strand, within b1772 at 1854.651 kb on + strand, within b1772 at 1854.652 kb on + strand, within b1772 at 1854.670 kb on + strand, within b1772 at 1854.670 kb on + strand, within b1772 at 1854.935 kb on - strand at 1854.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep1_HD100_no_dilution remove 1,852,035 + +0.4 1,852,163 - -1.1 1,852,165 - -0.5 1,852,165 - +0.5 1,852,165 - +0.7 1,852,167 - +1.0 1,852,172 - +0.1 1,852,224 - ydjF b1770 0.14 -0.2 1,852,252 - ydjF b1770 0.17 -0.7 1,852,252 - ydjF b1770 0.17 -0.2 1,852,252 - ydjF b1770 0.17 +0.6 1,852,252 - ydjF b1770 0.17 +0.1 1,852,252 - ydjF b1770 0.17 +0.8 1,852,264 - ydjF b1770 0.19 +0.5 1,852,264 - ydjF b1770 0.19 +0.5 1,852,388 - ydjF b1770 0.35 +0.1 1,852,457 - ydjF b1770 0.44 +0.2 1,852,527 + ydjF b1770 0.54 +0.4 1,852,535 - ydjF b1770 0.55 +0.7 1,852,535 - ydjF b1770 0.55 +1.4 1,852,535 - ydjF b1770 0.55 +0.1 1,852,576 - ydjF b1770 0.60 +0.2 1,852,581 - ydjF b1770 0.61 +0.9 1,852,606 + ydjF b1770 0.64 +0.8 1,852,611 - ydjF b1770 0.65 +0.6 1,852,636 - ydjF b1770 0.68 +0.6 1,852,853 + -0.8 1,853,029 - +0.3 1,853,029 - -0.9 1,853,048 - -0.3 1,853,102 + +0.9 1,853,110 - -0.1 1,853,170 + ydjG b1771 0.16 +1.2 1,853,192 - ydjG b1771 0.18 +0.0 1,853,368 - ydjG b1771 0.36 +0.7 1,853,425 - ydjG b1771 0.42 +1.2 1,853,425 - ydjG b1771 0.42 +0.9 1,853,448 + ydjG b1771 0.44 +0.8 1,853,537 - ydjG b1771 0.53 +0.2 1,853,621 - ydjG b1771 0.62 +0.7 1,853,621 - ydjG b1771 0.62 +1.5 1,853,666 - ydjG b1771 0.66 +0.1 1,853,666 - ydjG b1771 0.66 -1.6 1,853,751 + ydjG b1771 0.75 +2.8 1,853,763 - ydjG b1771 0.76 +1.2 1,853,919 - +0.1 1,853,937 - +0.1 1,853,947 - +1.0 1,854,143 - b1772 b1772 0.15 -0.4 1,854,143 - b1772 b1772 0.15 -0.9 1,854,178 - b1772 b1772 0.18 -0.6 1,854,178 - b1772 b1772 0.18 +0.1 1,854,223 - b1772 b1772 0.23 -0.8 1,854,246 - b1772 b1772 0.25 -0.2 1,854,321 - b1772 b1772 0.33 -2.6 1,854,389 + b1772 b1772 0.41 +0.8 1,854,397 - b1772 b1772 0.41 -0.2 1,854,425 - b1772 b1772 0.44 -1.7 1,854,425 - b1772 b1772 0.44 -1.2 1,854,461 - b1772 b1772 0.48 -0.2 1,854,461 - b1772 b1772 0.48 -1.2 1,854,550 - b1772 b1772 0.57 +0.8 1,854,644 - b1772 b1772 0.67 -0.8 1,854,644 - b1772 b1772 0.67 -0.8 1,854,651 + b1772 b1772 0.68 +0.8 1,854,652 + b1772 b1772 0.68 +0.8 1,854,670 + b1772 b1772 0.70 +0.8 1,854,670 + b1772 b1772 0.70 -0.8 1,854,935 - +0.8 1,854,935 - +0.8
Or see this region's nucleotide sequence