Experiment: 48 hr rep1_HD100_no_dilution
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insH-5 and b1372 are separated by 322 nucleotides b1372 and tfaR overlap by 1 nucleotides tfaR and pinR are separated by 97 nucleotides
b1370: insH-5 - Rac prophage; IS5 transposase and trans-activator (NCBI), at 1,425,770 to 1,426,750
insH-5
b1372: b1372 - putative membrane protein (VIMSS), at 1,427,073 to 1,430,435
b1372
b1373: tfaR - Rac prophage; predicted tail fiber assembly protein (NCBI), at 1,430,435 to 1,431,010
tfaR
b1374: pinR - Rac prophage; predicted site-specific recombinase (NCBI), at 1,431,108 to 1,431,698
pinR
Position (kb)
1427
1428
1429
1430
1431 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1426.405 kb on + strand, within insH-5 at 1426.456 kb on - strand, within insH-5 at 1426.658 kb on + strand at 1426.658 kb on + strand at 1426.738 kb on + strand at 1426.738 kb on + strand at 1426.746 kb on + strand at 1426.877 kb on + strand at 1426.954 kb on - strand at 1426.954 kb on - strand at 1427.144 kb on - strand at 1427.251 kb on - strand at 1427.764 kb on - strand, within b1372 at 1427.764 kb on - strand, within b1372 at 1427.937 kb on + strand, within b1372 at 1428.125 kb on + strand, within b1372 at 1428.482 kb on + strand, within b1372 at 1428.485 kb on - strand, within b1372 at 1428.485 kb on - strand, within b1372 at 1428.506 kb on + strand, within b1372 at 1428.633 kb on - strand, within b1372 at 1428.633 kb on - strand, within b1372 at 1428.681 kb on - strand, within b1372 at 1428.681 kb on - strand, within b1372 at 1428.751 kb on + strand, within b1372 at 1428.807 kb on - strand, within b1372 at 1428.972 kb on + strand, within b1372 at 1429.180 kb on - strand, within b1372 at 1429.180 kb on - strand, within b1372 at 1429.248 kb on + strand, within b1372 at 1429.418 kb on + strand, within b1372 at 1429.429 kb on + strand, within b1372 at 1429.429 kb on + strand, within b1372 at 1429.526 kb on - strand, within b1372 at 1429.542 kb on - strand, within b1372 at 1429.679 kb on + strand, within b1372 at 1429.832 kb on - strand, within b1372 at 1430.281 kb on + strand at 1431.253 kb on - strand, within pinR
Per-strain Table
Position Strand Gene LocusTag Fraction 48 hr rep1_HD100_no_dilution remove 1,426,405 + insH-5 b1370 0.65 -1.9 1,426,456 - insH-5 b1370 0.70 -0.5 1,426,658 + -0.5 1,426,658 + +0.2 1,426,738 + +1.3 1,426,738 + -0.1 1,426,746 + +1.6 1,426,877 + +0.3 1,426,954 - -0.5 1,426,954 - -0.2 1,427,144 - +0.2 1,427,251 - -0.4 1,427,764 - b1372 b1372 0.21 +0.1 1,427,764 - b1372 b1372 0.21 -0.0 1,427,937 + b1372 b1372 0.26 +0.8 1,428,125 + b1372 b1372 0.31 +0.7 1,428,482 + b1372 b1372 0.42 +0.4 1,428,485 - b1372 b1372 0.42 +0.7 1,428,485 - b1372 b1372 0.42 +0.1 1,428,506 + b1372 b1372 0.43 +1.0 1,428,633 - b1372 b1372 0.46 +0.7 1,428,633 - b1372 b1372 0.46 +1.1 1,428,681 - b1372 b1372 0.48 -1.1 1,428,681 - b1372 b1372 0.48 -2.0 1,428,751 + b1372 b1372 0.50 -1.4 1,428,807 - b1372 b1372 0.52 +0.1 1,428,972 + b1372 b1372 0.56 +0.5 1,429,180 - b1372 b1372 0.63 +1.2 1,429,180 - b1372 b1372 0.63 +0.3 1,429,248 + b1372 b1372 0.65 +0.1 1,429,418 + b1372 b1372 0.70 +2.8 1,429,429 + b1372 b1372 0.70 +1.4 1,429,429 + b1372 b1372 0.70 +1.3 1,429,526 - b1372 b1372 0.73 +2.0 1,429,542 - b1372 b1372 0.73 -0.2 1,429,679 + b1372 b1372 0.77 +0.2 1,429,832 - b1372 b1372 0.82 +1.2 1,430,281 + +0.3 1,431,253 - pinR b1374 0.25 +3.1
Or see this region's nucleotide sequence