Experiment: Putida_ML5X with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4056 and PP_4057 are separated by 316 nucleotides PP_4057 and PP_4058 are separated by 108 nucleotides
PP_4056: PP_4056 - putative phosphohydrolase of unknown function, at 4,575,604 to 4,576,398
_4056
PP_4057: PP_4057 - conserved membrane protein of unknown function with autotransporter beta-domain, at 4,576,715 to 4,577,791
_4057
PP_4058: PP_4058 - 1,4-alpha-glucan branching enzyme, at 4,577,900 to 4,580,110
_4058
Position (kb)
4576
4577
4578 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4575.723 kb on + strand, within PP_4056 at 4575.757 kb on - strand, within PP_4056 at 4575.757 kb on - strand, within PP_4056 at 4575.905 kb on - strand, within PP_4056 at 4575.943 kb on + strand at 4576.110 kb on + strand, within PP_4056 at 4576.295 kb on + strand, within PP_4056 at 4576.295 kb on + strand, within PP_4056 at 4576.296 kb on - strand, within PP_4056 at 4576.296 kb on - strand, within PP_4056 at 4576.296 kb on - strand, within PP_4056 at 4576.297 kb on + strand, within PP_4056 at 4576.297 kb on + strand, within PP_4056 at 4576.298 kb on - strand, within PP_4056 at 4576.298 kb on - strand, within PP_4056 at 4576.412 kb on + strand at 4576.413 kb on - strand at 4576.544 kb on - strand at 4576.544 kb on - strand at 4576.544 kb on - strand at 4576.544 kb on - strand at 4576.554 kb on + strand at 4576.586 kb on + strand at 4576.587 kb on - strand at 4576.808 kb on + strand at 4577.228 kb on + strand, within PP_4057 at 4577.228 kb on + strand, within PP_4057 at 4577.229 kb on - strand, within PP_4057 at 4577.271 kb on + strand, within PP_4057 at 4577.558 kb on + strand at 4577.558 kb on + strand, within PP_4057 at 4577.558 kb on + strand, within PP_4057 at 4577.567 kb on - strand, within PP_4057 at 4577.721 kb on - strand at 4577.789 kb on + strand at 4577.815 kb on - strand at 4577.827 kb on - strand at 4577.859 kb on - strand at 4577.887 kb on + strand at 4578.024 kb on + strand at 4578.025 kb on - strand at 4578.166 kb on - strand, within PP_4058 at 4578.303 kb on + strand, within PP_4058 at 4578.315 kb on + strand, within PP_4058 at 4578.417 kb on + strand, within PP_4058 at 4578.418 kb on - strand, within PP_4058 at 4578.418 kb on - strand, within PP_4058 at 4578.537 kb on + strand at 4578.537 kb on + strand, within PP_4058 at 4578.538 kb on - strand, within PP_4058 at 4578.538 kb on - strand, within PP_4058 at 4578.538 kb on - strand, within PP_4058 at 4578.538 kb on - strand, within PP_4058 at 4578.570 kb on + strand, within PP_4058 at 4578.570 kb on + strand, within PP_4058 at 4578.571 kb on - strand, within PP_4058 at 4578.600 kb on + strand, within PP_4058 at 4578.600 kb on + strand, within PP_4058 at 4578.600 kb on + strand, within PP_4058 at 4578.600 kb on + strand, within PP_4058 at 4578.601 kb on - strand, within PP_4058 at 4578.601 kb on - strand, within PP_4058 at 4578.601 kb on - strand, within PP_4058 at 4578.639 kb on + strand, within PP_4058
Per-strain Table
Position Strand Gene LocusTag Fraction Putida_ML5X with Paraburkholderia sabiae T6SS mutant remove 4,575,723 + PP_4056 0.15 +0.2 4,575,757 - PP_4056 0.19 +0.1 4,575,757 - PP_4056 0.19 +0.3 4,575,905 - PP_4056 0.38 -0.7 4,575,943 + +0.6 4,576,110 + PP_4056 0.64 +0.3 4,576,295 + PP_4056 0.87 -0.8 4,576,295 + PP_4056 0.87 +1.2 4,576,296 - PP_4056 0.87 +0.0 4,576,296 - PP_4056 0.87 +0.1 4,576,296 - PP_4056 0.87 +0.2 4,576,297 + PP_4056 0.87 +0.9 4,576,297 + PP_4056 0.87 +0.8 4,576,298 - PP_4056 0.87 +0.7 4,576,298 - PP_4056 0.87 +0.2 4,576,412 + -1.1 4,576,413 - -0.5 4,576,544 - +0.6 4,576,544 - +0.6 4,576,544 - +1.1 4,576,544 - +0.2 4,576,554 + +0.0 4,576,586 + -1.2 4,576,587 - -0.1 4,576,808 + +0.8 4,577,228 + PP_4057 0.48 -0.3 4,577,228 + PP_4057 0.48 -1.0 4,577,229 - PP_4057 0.48 -1.3 4,577,271 + PP_4057 0.52 -0.2 4,577,558 + -1.1 4,577,558 + PP_4057 0.78 -0.5 4,577,558 + PP_4057 0.78 -1.7 4,577,567 - PP_4057 0.79 +0.3 4,577,721 - +1.1 4,577,789 + +1.1 4,577,815 - +1.2 4,577,827 - +0.5 4,577,859 - +0.2 4,577,887 + +1.5 4,578,024 + -0.2 4,578,025 - -1.4 4,578,166 - PP_4058 0.12 +1.2 4,578,303 + PP_4058 0.18 -0.9 4,578,315 + PP_4058 0.19 -1.9 4,578,417 + PP_4058 0.23 +0.2 4,578,418 - PP_4058 0.23 -0.0 4,578,418 - PP_4058 0.23 -3.2 4,578,537 + -1.6 4,578,537 + PP_4058 0.29 +0.7 4,578,538 - PP_4058 0.29 +1.5 4,578,538 - PP_4058 0.29 -1.2 4,578,538 - PP_4058 0.29 +0.2 4,578,538 - PP_4058 0.29 +0.7 4,578,570 + PP_4058 0.30 +1.0 4,578,570 + PP_4058 0.30 -0.7 4,578,571 - PP_4058 0.30 -0.1 4,578,600 + PP_4058 0.32 -1.6 4,578,600 + PP_4058 0.32 -2.8 4,578,600 + PP_4058 0.32 -1.4 4,578,600 + PP_4058 0.32 -2.4 4,578,601 - PP_4058 0.32 -0.7 4,578,601 - PP_4058 0.32 +0.2 4,578,601 - PP_4058 0.32 -3.1 4,578,639 + PP_4058 0.33 -1.1
Or see this region's nucleotide sequence