Experiment: Putida_ML5X with Paraburkholderia sabiae T6SS mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0021 and PP_0022 are separated by 184 nucleotides PP_0022 and PP_0023 are separated by 445 nucleotides PP_0023 and PP_0024 are separated by 122 nucleotides
PP_0021: PP_0021 - conserved hypothetical protein, at 25,881 to 26,804
_0021
PP_0022: PP_0022 - hypothetical protein, at 26,989 to 27,153
_0022
PP_0023: PP_0023 - conserved hypothetical protein, at 27,599 to 27,727
_0023
PP_0024: PP_0024 - putative membrane-associated metal-dependent hydrolase, at 27,850 to 29,547
_0024
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 25.999 kb on + strand, within PP_0021 at 25.999 kb on + strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.015 kb on - strand, within PP_0021 at 26.015 kb on - strand, within PP_0021 at 26.056 kb on - strand, within PP_0021 at 26.062 kb on + strand, within PP_0021 at 26.062 kb on + strand, within PP_0021 at 26.063 kb on - strand, within PP_0021 at 26.063 kb on - strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.100 kb on - strand, within PP_0021 at 26.100 kb on - strand, within PP_0021 at 26.100 kb on - strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.132 kb on + strand, within PP_0021 at 26.132 kb on + strand, within PP_0021 at 26.158 kb on - strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.234 kb on - strand, within PP_0021 at 26.234 kb on - strand, within PP_0021 at 26.262 kb on + strand, within PP_0021 at 26.263 kb on - strand, within PP_0021 at 26.328 kb on + strand, within PP_0021 at 26.328 kb on + strand, within PP_0021 at 26.328 kb on + strand, within PP_0021 at 26.346 kb on + strand, within PP_0021 at 26.346 kb on + strand, within PP_0021 at 26.352 kb on + strand, within PP_0021 at 26.352 kb on + strand, within PP_0021 at 26.352 kb on + strand, within PP_0021 at 26.353 kb on - strand, within PP_0021 at 26.353 kb on - strand, within PP_0021 at 26.355 kb on - strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.459 kb on + strand, within PP_0021 at 26.459 kb on + strand, within PP_0021 at 26.459 kb on + strand, within PP_0021 at 26.460 kb on - strand, within PP_0021 at 26.517 kb on + strand, within PP_0021 at 26.542 kb on - strand, within PP_0021 at 26.626 kb on + strand, within PP_0021 at 26.626 kb on + strand, within PP_0021 at 26.627 kb on - strand, within PP_0021 at 26.627 kb on - strand, within PP_0021 at 26.627 kb on - strand, within PP_0021 at 26.733 kb on + strand at 26.734 kb on - strand at 26.747 kb on + strand at 26.747 kb on + strand at 26.818 kb on - strand at 26.837 kb on + strand at 26.838 kb on - strand at 26.851 kb on + strand at 26.851 kb on + strand at 26.851 kb on + strand at 26.852 kb on - strand at 26.871 kb on + strand at 26.878 kb on + strand at 26.879 kb on - strand at 26.879 kb on - strand at 26.882 kb on + strand at 26.882 kb on + strand at 26.883 kb on - strand at 26.955 kb on + strand at 26.955 kb on + strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.991 kb on - strand at 26.992 kb on + strand at 26.993 kb on - strand at 26.993 kb on - strand at 26.993 kb on - strand at 27.091 kb on - strand, within PP_0022 at 27.140 kb on + strand at 27.140 kb on + strand at 27.141 kb on - strand at 27.173 kb on - strand at 27.211 kb on - strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.321 kb on + strand at 27.322 kb on - strand at 27.322 kb on - strand at 27.322 kb on - strand at 27.432 kb on + strand at 27.433 kb on - strand at 27.462 kb on - strand at 27.476 kb on + strand at 27.476 kb on + strand at 27.600 kb on + strand at 27.601 kb on - strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.767 kb on - strand at 27.767 kb on - strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.771 kb on - strand at 27.771 kb on - strand at 27.774 kb on + strand at 27.775 kb on - strand at 27.775 kb on - strand at 27.863 kb on + strand at 27.863 kb on + strand at 27.864 kb on - strand at 27.864 kb on - strand at 27.864 kb on - strand at 27.885 kb on + strand at 27.885 kb on + strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.917 kb on + strand at 28.054 kb on + strand, within PP_0024 at 28.055 kb on - strand, within PP_0024 at 28.055 kb on - strand, within PP_0024 at 28.094 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.112 kb on + strand, within PP_0024 at 28.113 kb on - strand, within PP_0024 at 28.113 kb on - strand, within PP_0024
Per-strain Table
Position Strand Gene LocusTag Fraction Putida_ML5X with Paraburkholderia sabiae T6SS mutant remove 25,999 + PP_0021 0.13 +0.3 25,999 + PP_0021 0.13 -0.0 26,000 - PP_0021 0.13 -0.7 26,000 - PP_0021 0.13 -1.7 26,000 - PP_0021 0.13 +1.5 26,000 - PP_0021 0.13 -2.3 26,000 - PP_0021 0.13 -1.8 26,000 - PP_0021 0.13 -0.6 26,000 - PP_0021 0.13 +0.5 26,015 - PP_0021 0.15 +0.9 26,015 - PP_0021 0.15 +0.6 26,056 - PP_0021 0.19 -0.7 26,062 + PP_0021 0.20 -1.9 26,062 + PP_0021 0.20 +0.3 26,063 - PP_0021 0.20 -1.4 26,063 - PP_0021 0.20 +0.0 26,099 + PP_0021 0.24 -0.6 26,099 + PP_0021 0.24 +0.2 26,099 + PP_0021 0.24 -0.7 26,099 + PP_0021 0.24 -1.5 26,099 + PP_0021 0.24 +0.8 26,100 - PP_0021 0.24 -1.5 26,100 - PP_0021 0.24 -0.8 26,100 - PP_0021 0.24 -1.0 26,102 + PP_0021 0.24 +0.6 26,102 + PP_0021 0.24 -0.2 26,102 + PP_0021 0.24 +1.4 26,102 + PP_0021 0.24 -0.9 26,103 - PP_0021 0.24 +0.7 26,103 - PP_0021 0.24 +0.9 26,103 - PP_0021 0.24 -0.4 26,103 - PP_0021 0.24 +1.6 26,103 - PP_0021 0.24 +1.8 26,132 + PP_0021 0.27 +1.2 26,132 + PP_0021 0.27 +0.0 26,158 - PP_0021 0.30 -1.1 26,233 + PP_0021 0.38 +0.5 26,233 + PP_0021 0.38 -1.5 26,233 + PP_0021 0.38 +0.8 26,233 + PP_0021 0.38 -2.3 26,234 - PP_0021 0.38 +0.4 26,234 - PP_0021 0.38 -0.3 26,262 + PP_0021 0.41 +2.2 26,263 - PP_0021 0.41 -0.5 26,328 + PP_0021 0.48 +0.1 26,328 + PP_0021 0.48 -1.9 26,328 + PP_0021 0.48 +0.2 26,346 + PP_0021 0.50 +0.0 26,346 + PP_0021 0.50 +0.3 26,352 + PP_0021 0.51 -1.5 26,352 + PP_0021 0.51 -0.3 26,352 + PP_0021 0.51 +0.0 26,353 - PP_0021 0.51 +0.9 26,353 - PP_0021 0.51 -0.3 26,355 - PP_0021 0.51 +0.9 26,394 + PP_0021 0.56 -0.3 26,394 + PP_0021 0.56 -0.1 26,394 + PP_0021 0.56 -0.1 26,394 + PP_0021 0.56 +1.6 26,394 + PP_0021 0.56 -1.6 26,394 + PP_0021 0.56 +0.0 26,394 + PP_0021 0.56 -0.1 26,394 + PP_0021 0.56 +0.9 26,394 + PP_0021 0.56 +0.0 26,394 + PP_0021 0.56 -0.0 26,394 + PP_0021 0.56 -2.9 26,394 + PP_0021 0.56 -1.6 26,395 - PP_0021 0.56 -0.5 26,395 - PP_0021 0.56 +1.8 26,395 - PP_0021 0.56 -3.1 26,395 - -0.8 26,395 - PP_0021 0.56 +0.4 26,395 - PP_0021 0.56 +0.8 26,395 - PP_0021 0.56 -0.5 26,395 - PP_0021 0.56 +0.2 26,395 - PP_0021 0.56 +1.1 26,459 + PP_0021 0.63 +1.1 26,459 + PP_0021 0.63 -0.6 26,459 + PP_0021 0.63 -2.3 26,460 - PP_0021 0.63 -0.4 26,517 + PP_0021 0.69 +1.0 26,542 - PP_0021 0.72 +0.2 26,626 + PP_0021 0.81 +1.7 26,626 + PP_0021 0.81 +0.0 26,627 - PP_0021 0.81 -0.3 26,627 - PP_0021 0.81 +0.7 26,627 - PP_0021 0.81 +0.4 26,733 + -0.5 26,734 - -0.2 26,747 + +2.8 26,747 + -3.2 26,818 - +0.2 26,837 + +1.1 26,838 - +1.1 26,851 + -0.4 26,851 + -1.5 26,851 + +0.4 26,852 - +0.5 26,871 + -3.3 26,878 + -0.9 26,879 - +0.1 26,879 - +0.3 26,882 + -1.4 26,882 + -2.2 26,883 - -1.5 26,955 + -1.2 26,955 + +1.1 26,956 - +0.1 26,956 - -0.1 26,956 - +0.5 26,956 - -0.6 26,956 - -1.1 26,956 - +1.2 26,956 - +0.6 26,990 + +0.7 26,990 + +1.0 26,990 + -1.5 26,990 + +0.2 26,991 - -0.4 26,992 + -1.9 26,993 - -0.6 26,993 - -0.4 26,993 - -0.6 27,091 - PP_0022 0.62 -3.3 27,140 + +0.5 27,140 + +1.7 27,141 - -3.0 27,173 - -0.2 27,211 - +0.7 27,291 + +0.6 27,291 + +0.4 27,291 + +1.1 27,291 + -0.1 27,291 + -2.6 27,291 + +0.1 27,291 + +1.6 27,291 + +0.6 27,291 + +0.4 27,292 - -0.1 27,292 - -0.8 27,292 - -0.4 27,292 - -0.5 27,292 - -2.3 27,292 - -0.5 27,292 - +0.3 27,292 - +1.5 27,292 - +0.4 27,292 - -0.7 27,292 - -0.5 27,292 - +0.2 27,292 - -0.5 27,292 - -2.6 27,292 - -0.4 27,321 + -0.4 27,322 - -2.9 27,322 - +1.6 27,322 - +1.4 27,432 + +0.2 27,433 - +0.5 27,462 - -0.3 27,476 + -1.1 27,476 + -1.5 27,600 + +0.6 27,601 - -1.0 27,766 + +0.0 27,766 + +0.3 27,766 + -0.7 27,766 + -0.9 27,767 - -1.8 27,767 - +0.5 27,770 + +1.1 27,770 + -0.2 27,770 + -0.3 27,770 + -3.3 27,770 + -0.4 27,771 - -1.5 27,771 - +1.5 27,774 + +0.1 27,775 - +0.5 27,775 - +0.0 27,863 + -0.2 27,863 + +0.3 27,864 - +2.2 27,864 - +2.0 27,864 - +1.2 27,885 + +0.9 27,885 + -1.1 27,886 - +0.5 27,886 - -1.2 27,886 - +0.7 27,886 - +0.3 27,886 - -0.6 27,917 + -2.5 28,054 + PP_0024 0.12 +0.3 28,055 - PP_0024 0.12 +0.6 28,055 - PP_0024 0.12 -0.4 28,094 + PP_0024 0.14 -0.6 28,108 + PP_0024 0.15 +0.9 28,108 + PP_0024 0.15 -0.8 28,108 + PP_0024 0.15 -2.0 28,108 + PP_0024 0.15 +0.3 28,109 - PP_0024 0.15 -0.7 28,109 - PP_0024 0.15 +0.7 28,109 - PP_0024 0.15 -1.0 28,112 + PP_0024 0.15 +0.2 28,113 - PP_0024 0.15 -0.3 28,113 - PP_0024 0.15 +0.1
Or see this region's nucleotide sequence