Experiment: R2_N4_T2_phage10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdtI and mdtJ overlap by 14 nucleotides mdtJ and ydgG are separated by 411 nucleotides ydgG and pntB are separated by 24 nucleotides
b1599: mdtI - multidrug efflux system transporter (NCBI), at 1,670,844 to 1,671,173
mdtI
b1600: mdtJ - multidrug efflux system transporter (NCBI), at 1,671,160 to 1,671,525
mdtJ
b1601: ydgG - predicted inner membrane protein (NCBI), at 1,671,937 to 1,672,971
ydgG
b1602: pntB - pyridine nucleotide transhydrogenase (NCBI), at 1,672,996 to 1,674,384
pntB
Position (kb)
1671
1672
1673 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1671.156 kb on - strand at 1671.156 kb on - strand at 1671.165 kb on - strand at 1671.182 kb on - strand at 1671.186 kb on + strand at 1671.251 kb on - strand, within mdtJ at 1671.253 kb on - strand, within mdtJ at 1671.346 kb on + strand, within mdtJ at 1671.348 kb on - strand, within mdtJ at 1671.348 kb on - strand, within mdtJ at 1671.354 kb on - strand, within mdtJ at 1671.354 kb on - strand, within mdtJ at 1671.355 kb on - strand, within mdtJ at 1671.449 kb on - strand, within mdtJ at 1671.466 kb on - strand, within mdtJ at 1671.466 kb on - strand, within mdtJ at 1671.474 kb on - strand, within mdtJ at 1671.525 kb on - strand at 1671.595 kb on - strand at 1671.615 kb on - strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.653 kb on - strand at 1671.665 kb on + strand at 1671.761 kb on - strand at 1671.761 kb on - strand at 1671.761 kb on - strand at 1671.800 kb on - strand at 1671.800 kb on - strand at 1671.927 kb on + strand at 1671.935 kb on + strand at 1671.941 kb on - strand at 1671.943 kb on - strand at 1672.027 kb on + strand at 1672.082 kb on - strand, within ydgG at 1672.082 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.095 kb on - strand, within ydgG at 1672.102 kb on - strand, within ydgG at 1672.106 kb on + strand, within ydgG at 1672.151 kb on - strand, within ydgG at 1672.157 kb on - strand, within ydgG at 1672.291 kb on - strand, within ydgG at 1672.365 kb on + strand, within ydgG at 1672.365 kb on + strand, within ydgG at 1672.413 kb on + strand, within ydgG at 1672.481 kb on + strand, within ydgG at 1672.668 kb on - strand, within ydgG at 1672.698 kb on - strand, within ydgG at 1672.698 kb on - strand, within ydgG at 1672.796 kb on + strand, within ydgG at 1673.042 kb on + strand at 1673.095 kb on - strand at 1673.102 kb on - strand at 1673.145 kb on + strand, within pntB at 1673.501 kb on - strand, within pntB at 1673.579 kb on - strand, within pntB at 1673.579 kb on - strand, within pntB at 1673.667 kb on + strand, within pntB at 1673.672 kb on + strand, within pntB at 1673.672 kb on + strand, within pntB at 1673.878 kb on + strand, within pntB at 1673.878 kb on + strand, within pntB
Per-strain Table
Position Strand Gene LocusTag Fraction R2_N4_T2_phage10 remove 1,671,156 - -1.0 1,671,156 - +0.9 1,671,165 - +1.4 1,671,182 - +1.0 1,671,186 + -1.5 1,671,251 - mdtJ b1600 0.25 -1.5 1,671,253 - mdtJ b1600 0.25 -0.5 1,671,346 + mdtJ b1600 0.51 -0.5 1,671,348 - mdtJ b1600 0.51 -0.5 1,671,348 - mdtJ b1600 0.51 -0.5 1,671,354 - mdtJ b1600 0.53 -2.1 1,671,354 - mdtJ b1600 0.53 -0.5 1,671,355 - mdtJ b1600 0.53 -2.5 1,671,449 - mdtJ b1600 0.79 -0.5 1,671,466 - mdtJ b1600 0.84 -1.5 1,671,466 - mdtJ b1600 0.84 -0.5 1,671,474 - mdtJ b1600 0.86 -0.5 1,671,525 - -0.5 1,671,595 - +1.1 1,671,615 - -3.1 1,671,653 - -3.3 1,671,653 - +4.8 1,671,653 - +2.7 1,671,653 - +0.7 1,671,665 + -0.5 1,671,761 - +1.6 1,671,761 - -0.6 1,671,761 - -0.8 1,671,800 - -3.7 1,671,800 - +1.4 1,671,927 + -0.5 1,671,935 + -0.5 1,671,941 - -2.5 1,671,943 - -0.5 1,672,027 + +2.5 1,672,082 - ydgG b1601 0.14 -1.5 1,672,082 - ydgG b1601 0.14 -2.5 1,672,095 - ydgG b1601 0.15 -1.5 1,672,095 - ydgG b1601 0.15 -2.5 1,672,095 - ydgG b1601 0.15 -2.1 1,672,102 - ydgG b1601 0.16 -0.7 1,672,106 + ydgG b1601 0.16 -2.5 1,672,151 - ydgG b1601 0.21 -1.4 1,672,157 - ydgG b1601 0.21 +1.4 1,672,291 - ydgG b1601 0.34 -0.5 1,672,365 + ydgG b1601 0.41 +0.4 1,672,365 + ydgG b1601 0.41 -0.3 1,672,413 + ydgG b1601 0.46 -1.7 1,672,481 + ydgG b1601 0.53 -1.1 1,672,668 - ydgG b1601 0.71 -2.2 1,672,698 - ydgG b1601 0.74 -0.9 1,672,698 - ydgG b1601 0.74 -3.1 1,672,796 + ydgG b1601 0.83 +1.7 1,673,042 + +0.2 1,673,095 - -0.3 1,673,102 - +1.9 1,673,145 + pntB b1602 0.11 -2.6 1,673,501 - pntB b1602 0.36 -0.2 1,673,579 - pntB b1602 0.42 +1.5 1,673,579 - pntB b1602 0.42 +0.6 1,673,667 + pntB b1602 0.48 +0.7 1,673,672 + pntB b1602 0.49 +0.0 1,673,672 + pntB b1602 0.49 +0.8 1,673,878 + pntB b1602 0.63 +0.9 1,673,878 + pntB b1602 0.63 -1.2
Or see this region's nucleotide sequence