Strain Fitness in Escherichia coli BW25113 around b3821

Experiment: R2_N4_T1_phage10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrarD and yigI are separated by 51 nucleotidesyigI and pldA are separated by 164 nucleotidespldA and recQ are separated by 132 nucleotides b3819: rarD - predicted chloramphenical resistance permease (NCBI), at 4,001,311 to 4,002,201 rarD b3820: yigI - orf, hypothetical protein (VIMSS), at 4,002,253 to 4,002,720 yigI b3821: pldA - outer membrane phospholipase A (NCBI), at 4,002,885 to 4,003,754 pldA b3822: recQ - ATP-dependent DNA helicase (NCBI), at 4,003,887 to 4,005,716 recQ Position (kb) 4002 4003 4004Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 4001.898 kb on + strand, within rarDat 4001.898 kb on + strand, within rarDat 4001.899 kb on + strand, within rarDat 4001.917 kb on + strand, within rarDat 4001.917 kb on - strand, within rarDat 4001.917 kb on - strand, within rarDat 4001.920 kb on - strand, within rarDat 4001.929 kb on + strand, within rarDat 4001.929 kb on + strand, within rarDat 4001.929 kb on + strand, within rarDat 4001.929 kb on + strand, within rarDat 4001.929 kb on + strand, within rarDat 4001.929 kb on + strand, within rarDat 4001.929 kb on + strand, within rarDat 4001.937 kb on - strand, within rarDat 4001.937 kb on - strand, within rarDat 4001.937 kb on - strand, within rarDat 4001.939 kb on + strand, within rarDat 4001.950 kb on + strand, within rarDat 4001.953 kb on + strand, within rarDat 4001.953 kb on + strandat 4001.953 kb on + strandat 4001.956 kb on - strand, within rarDat 4001.956 kb on - strand, within rarDat 4001.959 kb on - strand, within rarDat 4001.961 kb on + strand, within rarDat 4001.961 kb on - strand, within rarDat 4001.961 kb on - strand, within rarDat 4001.961 kb on - strand, within rarDat 4001.961 kb on - strand, within rarDat 4001.961 kb on - strand, within rarDat 4001.961 kb on - strand, within rarDat 4001.962 kb on + strand, within rarDat 4001.962 kb on + strand, within rarDat 4001.962 kb on + strand, within rarDat 4001.963 kb on + strand, within rarDat 4001.970 kb on - strand, within rarDat 4002.004 kb on - strand, within rarDat 4002.004 kb on - strand, within rarDat 4002.009 kb on + strand, within rarDat 4002.035 kb on + strand, within rarDat 4002.035 kb on + strand, within rarDat 4002.038 kb on - strand, within rarDat 4002.040 kb on + strand, within rarDat 4002.043 kb on + strand, within rarDat 4002.050 kb on + strand, within rarDat 4002.051 kb on + strand, within rarDat 4002.051 kb on - strand, within rarDat 4002.051 kb on - strand, within rarDat 4002.051 kb on - strand, within rarDat 4002.051 kb on - strand, within rarDat 4002.051 kb on - strand, within rarDat 4002.064 kb on + strand, within rarDat 4002.064 kb on + strand, within rarDat 4002.087 kb on - strand, within rarDat 4002.115 kb on - strandat 4002.115 kb on - strandat 4002.118 kb on - strandat 4002.175 kb on + strandat 4002.176 kb on + strandat 4002.199 kb on + strandat 4002.199 kb on + strandat 4002.231 kb on - strandat 4002.256 kb on + strandat 4002.258 kb on + strandat 4002.270 kb on - strandat 4002.283 kb on + strandat 4002.283 kb on + strandat 4002.295 kb on - strandat 4002.295 kb on - strandat 4002.312 kb on - strand, within yigIat 4002.333 kb on + strand, within yigIat 4002.354 kb on + strand, within yigIat 4002.354 kb on + strand, within yigIat 4002.354 kb on + strand, within yigIat 4002.363 kb on - strand, within yigIat 4002.363 kb on - strand, within yigIat 4002.376 kb on - strand, within yigIat 4002.380 kb on + strand, within yigIat 4002.388 kb on - strand, within yigIat 4002.388 kb on - strand, within yigIat 4002.388 kb on - strand, within yigIat 4002.388 kb on - strand, within yigIat 4002.419 kb on + strand, within yigIat 4002.425 kb on + strandat 4002.430 kb on + strand, within yigIat 4002.441 kb on + strand, within yigIat 4002.441 kb on + strand, within yigIat 4002.450 kb on + strand, within yigIat 4002.450 kb on + strand, within yigIat 4002.475 kb on + strand, within yigIat 4002.483 kb on - strand, within yigIat 4002.485 kb on + strand, within yigIat 4002.509 kb on - strand, within yigIat 4002.519 kb on - strand, within yigIat 4002.526 kb on + strand, within yigIat 4002.614 kb on + strand, within yigIat 4002.622 kb on + strand, within yigIat 4002.622 kb on + strand, within yigIat 4002.622 kb on + strand, within yigIat 4002.622 kb on + strand, within yigIat 4002.627 kb on + strand, within yigIat 4002.627 kb on + strand, within yigIat 4002.630 kb on - strand, within yigIat 4002.660 kb on + strand, within yigIat 4002.678 kb on - strandat 4002.685 kb on + strandat 4002.693 kb on - strandat 4002.708 kb on + strandat 4002.708 kb on + strandat 4002.708 kb on + strandat 4002.725 kb on + strandat 4002.725 kb on + strandat 4002.725 kb on + strandat 4002.800 kb on + strandat 4002.803 kb on - strandat 4002.808 kb on - strandat 4002.808 kb on - strandat 4002.813 kb on + strandat 4002.874 kb on + strandat 4002.884 kb on + strandat 4002.893 kb on - strandat 4002.893 kb on - strandat 4002.894 kb on + strandat 4002.894 kb on + strandat 4002.900 kb on - strandat 4002.908 kb on - strandat 4002.915 kb on + strandat 4002.915 kb on + strandat 4002.924 kb on - strandat 4002.924 kb on - strandat 4002.940 kb on + strandat 4002.946 kb on - strandat 4002.957 kb on + strandat 4002.981 kb on - strand, within pldAat 4002.981 kb on - strand, within pldAat 4002.984 kb on + strand, within pldAat 4003.000 kb on - strand, within pldAat 4003.000 kb on - strand, within pldAat 4003.022 kb on + strand, within pldAat 4003.022 kb on + strand, within pldAat 4003.050 kb on - strand, within pldAat 4003.076 kb on + strand, within pldAat 4003.088 kb on + strand, within pldAat 4003.250 kb on - strand, within pldAat 4003.250 kb on - strand, within pldAat 4003.259 kb on + strand, within pldAat 4003.261 kb on + strand, within pldAat 4003.348 kb on + strand, within pldAat 4003.356 kb on - strand, within pldAat 4003.359 kb on + strand, within pldAat 4003.359 kb on + strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.367 kb on - strand, within pldAat 4003.389 kb on + strand, within pldAat 4003.412 kb on - strand, within pldAat 4003.415 kb on + strand, within pldAat 4003.415 kb on + strand, within pldAat 4003.472 kb on + strand, within pldAat 4003.472 kb on + strand, within pldAat 4003.476 kb on + strand, within pldAat 4003.477 kb on - strand, within pldAat 4003.477 kb on - strand, within pldAat 4003.480 kb on - strand, within pldAat 4003.484 kb on - strand, within pldAat 4003.484 kb on - strand, within pldAat 4003.489 kb on - strand, within pldAat 4003.512 kb on + strand, within pldAat 4003.512 kb on + strand, within pldAat 4003.586 kb on - strand, within pldAat 4003.614 kb on + strand, within pldAat 4003.614 kb on + strand, within pldAat 4003.616 kb on + strand, within pldAat 4003.620 kb on + strand, within pldAat 4003.632 kb on - strand, within pldAat 4003.632 kb on - strand, within pldAat 4003.660 kb on - strand, within pldAat 4003.723 kb on + strandat 4003.731 kb on - strandat 4003.735 kb on + strandat 4003.742 kb on - strandat 4003.993 kb on + strandat 4003.993 kb on + strandat 4003.995 kb on + strandat 4003.995 kb on + strandat 4004.003 kb on - strandat 4004.003 kb on - strandat 4004.028 kb on - strandat 4004.083 kb on + strand, within recQat 4004.133 kb on + strand, within recQat 4004.197 kb on + strand, within recQat 4004.374 kb on - strand, within recQat 4004.393 kb on - strand, within recQat 4004.436 kb on + strand, within recQat 4004.478 kb on - strand, within recQat 4004.535 kb on - strand, within recQat 4004.568 kb on - strandat 4004.573 kb on - strand, within recQat 4004.586 kb on - strand, within recQat 4004.586 kb on - strand, within recQat 4004.590 kb on + strand, within recQat 4004.632 kb on - strand, within recQat 4004.632 kb on - strand, within recQat 4004.651 kb on + strand, within recQat 4004.673 kb on - strand, within recQat 4004.740 kb on + strand, within recQat 4004.740 kb on + strand, within recQ

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Per-strain Table

Position Strand Gene LocusTag Fraction R2_N4_T1_phage10
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4,001,898 + rarD b3819 0.66 -2.6
4,001,898 + rarD b3819 0.66 -0.6
4,001,899 + rarD b3819 0.66 -0.6
4,001,917 + rarD b3819 0.68 +1.4
4,001,917 - rarD b3819 0.68 +0.4
4,001,917 - rarD b3819 0.68 +1.9
4,001,920 - rarD b3819 0.68 -0.5
4,001,929 + rarD b3819 0.69 -0.6
4,001,929 + rarD b3819 0.69 +1.4
4,001,929 + rarD b3819 0.69 +1.8
4,001,929 + rarD b3819 0.69 +1.8
4,001,929 + rarD b3819 0.69 +0.4
4,001,929 + rarD b3819 0.69 -0.7
4,001,929 + rarD b3819 0.69 +0.5
4,001,937 - rarD b3819 0.70 +0.4
4,001,937 - rarD b3819 0.70 -0.5
4,001,937 - rarD b3819 0.70 +0.3
4,001,939 + rarD b3819 0.70 -0.8
4,001,950 + rarD b3819 0.72 -2.6
4,001,953 + rarD b3819 0.72 -1.4
4,001,953 + +1.0
4,001,953 + +1.0
4,001,956 - rarD b3819 0.72 -0.1
4,001,956 - rarD b3819 0.72 -0.2
4,001,959 - rarD b3819 0.73 +0.7
4,001,961 + rarD b3819 0.73 -0.4
4,001,961 - rarD b3819 0.73 -3.3
4,001,961 - rarD b3819 0.73 -0.8
4,001,961 - rarD b3819 0.73 -0.9
4,001,961 - rarD b3819 0.73 +1.0
4,001,961 - rarD b3819 0.73 -1.4
4,001,961 - rarD b3819 0.73 +0.7
4,001,962 + rarD b3819 0.73 +0.7
4,001,962 + rarD b3819 0.73 -0.4
4,001,962 + rarD b3819 0.73 +1.2
4,001,963 + rarD b3819 0.73 -0.2
4,001,970 - rarD b3819 0.74 -0.2
4,002,004 - rarD b3819 0.78 +1.3
4,002,004 - rarD b3819 0.78 -2.3
4,002,009 + rarD b3819 0.78 -0.7
4,002,035 + rarD b3819 0.81 -0.9
4,002,035 + rarD b3819 0.81 +0.5
4,002,038 - rarD b3819 0.82 +0.1
4,002,040 + rarD b3819 0.82 +0.9
4,002,043 + rarD b3819 0.82 +1.5
4,002,050 + rarD b3819 0.83 +0.0
4,002,051 + rarD b3819 0.83 -2.1
4,002,051 - rarD b3819 0.83 -3.3
4,002,051 - rarD b3819 0.83 +0.1
4,002,051 - rarD b3819 0.83 -0.2
4,002,051 - rarD b3819 0.83 -1.0
4,002,051 - rarD b3819 0.83 +2.8
4,002,064 + rarD b3819 0.85 -0.3
4,002,064 + rarD b3819 0.85 +2.2
4,002,087 - rarD b3819 0.87 -2.2
4,002,115 - +0.6
4,002,115 - -1.8
4,002,118 - +1.4
4,002,175 + -0.1
4,002,176 + -0.0
4,002,199 + -1.2
4,002,199 + -1.8
4,002,231 - -0.9
4,002,256 + +0.4
4,002,258 + +1.7
4,002,270 - -3.9
4,002,283 + -1.0
4,002,283 + -0.1
4,002,295 - -0.3
4,002,295 - +1.5
4,002,312 - yigI b3820 0.13 -0.6
4,002,333 + yigI b3820 0.17 -1.3
4,002,354 + yigI b3820 0.22 +1.9
4,002,354 + yigI b3820 0.22 -0.6
4,002,354 + yigI b3820 0.22 +1.0
4,002,363 - yigI b3820 0.24 -0.4
4,002,363 - yigI b3820 0.24 -0.0
4,002,376 - yigI b3820 0.26 +1.5
4,002,380 + yigI b3820 0.27 -1.1
4,002,388 - yigI b3820 0.29 -0.6
4,002,388 - yigI b3820 0.29 +1.1
4,002,388 - yigI b3820 0.29 -0.5
4,002,388 - yigI b3820 0.29 -0.5
4,002,419 + yigI b3820 0.35 -0.3
4,002,425 + -0.1
4,002,430 + yigI b3820 0.38 -0.2
4,002,441 + yigI b3820 0.40 -1.3
4,002,441 + yigI b3820 0.40 +0.7
4,002,450 + yigI b3820 0.42 -1.1
4,002,450 + yigI b3820 0.42 -0.6
4,002,475 + yigI b3820 0.47 -2.4
4,002,483 - yigI b3820 0.49 +0.6
4,002,485 + yigI b3820 0.50 -0.9
4,002,509 - yigI b3820 0.55 +0.6
4,002,519 - yigI b3820 0.57 +0.1
4,002,526 + yigI b3820 0.58 +0.5
4,002,614 + yigI b3820 0.77 -0.2
4,002,622 + yigI b3820 0.79 -0.5
4,002,622 + yigI b3820 0.79 +0.2
4,002,622 + yigI b3820 0.79 -1.3
4,002,622 + yigI b3820 0.79 -2.4
4,002,627 + yigI b3820 0.80 +0.4
4,002,627 + yigI b3820 0.80 -0.6
4,002,630 - yigI b3820 0.81 -0.4
4,002,660 + yigI b3820 0.87 -0.1
4,002,678 - -1.6
4,002,685 + -1.4
4,002,693 - +0.8
4,002,708 + -1.7
4,002,708 + +2.0
4,002,708 + +1.0
4,002,725 + -1.6
4,002,725 + -2.4
4,002,725 + -1.0
4,002,800 + -0.1
4,002,803 - +0.0
4,002,808 - +4.3
4,002,808 - -2.3
4,002,813 + -0.6
4,002,874 + -2.0
4,002,884 + -1.2
4,002,893 - +0.5
4,002,893 - +1.7
4,002,894 + -0.2
4,002,894 + -2.5
4,002,900 - -0.9
4,002,908 - -2.7
4,002,915 + -2.3
4,002,915 + +3.1
4,002,924 - -1.8
4,002,924 - -1.9
4,002,940 + +3.3
4,002,946 - +0.0
4,002,957 + +1.2
4,002,981 - pldA b3821 0.11 -1.4
4,002,981 - pldA b3821 0.11 -3.6
4,002,984 + pldA b3821 0.11 -1.0
4,003,000 - pldA b3821 0.13 -0.6
4,003,000 - pldA b3821 0.13 +1.3
4,003,022 + pldA b3821 0.16 -0.1
4,003,022 + pldA b3821 0.16 +1.4
4,003,050 - pldA b3821 0.19 -0.6
4,003,076 + pldA b3821 0.22 -1.9
4,003,088 + pldA b3821 0.23 -2.5
4,003,250 - pldA b3821 0.42 +0.4
4,003,250 - pldA b3821 0.42 -0.8
4,003,259 + pldA b3821 0.43 +1.8
4,003,261 + pldA b3821 0.43 -0.2
4,003,348 + pldA b3821 0.53 -2.0
4,003,356 - pldA b3821 0.54 -1.1
4,003,359 + pldA b3821 0.54 +0.6
4,003,359 + pldA b3821 0.54 -2.9
4,003,367 - pldA b3821 0.55 +0.3
4,003,367 - pldA b3821 0.55 -1.5
4,003,367 - pldA b3821 0.55 -1.2
4,003,367 - pldA b3821 0.55 +0.8
4,003,389 + pldA b3821 0.58 +3.3
4,003,412 - pldA b3821 0.61 -1.8
4,003,415 + pldA b3821 0.61 +0.0
4,003,415 + pldA b3821 0.61 +1.4
4,003,472 + pldA b3821 0.67 +1.3
4,003,472 + pldA b3821 0.67 +0.5
4,003,476 + pldA b3821 0.68 -1.4
4,003,477 - pldA b3821 0.68 -0.8
4,003,477 - pldA b3821 0.68 -2.5
4,003,480 - pldA b3821 0.68 +0.4
4,003,484 - pldA b3821 0.69 -1.9
4,003,484 - pldA b3821 0.69 -0.1
4,003,489 - pldA b3821 0.69 -0.7
4,003,512 + pldA b3821 0.72 -0.1
4,003,512 + pldA b3821 0.72 +0.4
4,003,586 - pldA b3821 0.81 -0.1
4,003,614 + pldA b3821 0.84 -2.8
4,003,614 + pldA b3821 0.84 +0.4
4,003,616 + pldA b3821 0.84 -1.5
4,003,620 + pldA b3821 0.84 +0.2
4,003,632 - pldA b3821 0.86 +0.0
4,003,632 - pldA b3821 0.86 -1.0
4,003,660 - pldA b3821 0.89 +0.7
4,003,723 + +1.3
4,003,731 - -0.4
4,003,735 + +1.3
4,003,742 - -1.0
4,003,993 + -0.2
4,003,993 + +0.4
4,003,995 + -0.1
4,003,995 + -3.0
4,004,003 - +2.5
4,004,003 - +2.9
4,004,028 - -1.3
4,004,083 + recQ b3822 0.11 -3.1
4,004,133 + recQ b3822 0.13 -0.1
4,004,197 + recQ b3822 0.17 +2.5
4,004,374 - recQ b3822 0.27 -0.4
4,004,393 - recQ b3822 0.28 -3.0
4,004,436 + recQ b3822 0.30 -3.2
4,004,478 - recQ b3822 0.32 -0.4
4,004,535 - recQ b3822 0.35 +0.3
4,004,568 - +0.0
4,004,573 - recQ b3822 0.37 -1.6
4,004,586 - recQ b3822 0.38 +1.0
4,004,586 - recQ b3822 0.38 -0.2
4,004,590 + recQ b3822 0.38 -0.4
4,004,632 - recQ b3822 0.41 -1.2
4,004,632 - recQ b3822 0.41 -2.6
4,004,651 + recQ b3822 0.42 +0.5
4,004,673 - recQ b3822 0.43 -3.3
4,004,740 + recQ b3822 0.47 -0.2
4,004,740 + recQ b3822 0.47 -0.1

Or see this region's nucleotide sequence