Strain Fitness in Escherichia coli BW25113 around b2875

Experiment: R2_N4_T1_phage10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqeA and yqeB are separated by 220 nucleotidesyqeB and b2876 are separated by 47 nucleotidesb2876 and ygfJ are separated by 102 nucleotides b2874: yqeA - predicted amino acid kinase (NCBI), at 3,009,483 to 3,010,415 yqeA b2875: yqeB - conserved protein with NAD(P)-binding Rossman fold (NCBI), at 3,010,636 to 3,012,261 yqeB b2876: b2876 - orf, hypothetical protein (VIMSS), at 3,012,309 to 3,013,079 b2876 b2877: ygfJ - hypothetical protein (NCBI), at 3,013,182 to 3,013,760 ygfJ Position (kb) 3010 3011 3012 3013Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3009.654 kb on + strand, within yqeAat 3009.688 kb on + strand, within yqeAat 3009.701 kb on - strand, within yqeAat 3009.701 kb on - strand, within yqeAat 3009.730 kb on + strand, within yqeAat 3009.816 kb on - strand, within yqeAat 3009.822 kb on - strand, within yqeAat 3009.922 kb on - strand, within yqeAat 3010.002 kb on + strand, within yqeAat 3010.013 kb on + strand, within yqeAat 3010.232 kb on + strand, within yqeAat 3010.232 kb on + strand, within yqeAat 3010.280 kb on + strand, within yqeAat 3010.282 kb on + strand, within yqeAat 3010.282 kb on + strand, within yqeAat 3010.349 kb on + strandat 3010.390 kb on + strandat 3010.590 kb on + strandat 3010.596 kb on - strandat 3010.610 kb on + strandat 3010.618 kb on - strandat 3010.618 kb on - strandat 3010.697 kb on - strandat 3010.803 kb on + strand, within yqeBat 3010.822 kb on - strand, within yqeBat 3010.822 kb on - strand, within yqeBat 3010.914 kb on - strand, within yqeBat 3010.941 kb on + strand, within yqeBat 3010.952 kb on - strand, within yqeBat 3010.964 kb on - strand, within yqeBat 3010.980 kb on - strand, within yqeBat 3011.204 kb on - strand, within yqeBat 3011.209 kb on + strand, within yqeBat 3011.418 kb on - strand, within yqeBat 3011.469 kb on + strand, within yqeBat 3011.537 kb on + strand, within yqeBat 3011.560 kb on + strand, within yqeBat 3011.597 kb on - strand, within yqeBat 3011.616 kb on - strand, within yqeBat 3011.706 kb on - strand, within yqeBat 3011.918 kb on - strand, within yqeBat 3011.918 kb on - strand, within yqeBat 3011.971 kb on + strand, within yqeBat 3012.000 kb on - strand, within yqeBat 3012.020 kb on - strandat 3012.020 kb on - strand, within yqeBat 3012.022 kb on + strand, within yqeBat 3012.079 kb on - strand, within yqeBat 3012.079 kb on - strand, within yqeBat 3012.102 kb on - strandat 3012.147 kb on - strandat 3012.283 kb on - strandat 3012.304 kb on + strandat 3012.378 kb on + strandat 3012.497 kb on - strand, within b2876at 3012.666 kb on - strand, within b2876at 3012.666 kb on - strand, within b2876at 3012.715 kb on + strand, within b2876at 3012.848 kb on + strand, within b2876at 3012.888 kb on + strand, within b2876at 3012.888 kb on + strand, within b2876at 3012.939 kb on + strand, within b2876at 3012.947 kb on - strand, within b2876at 3012.954 kb on + strand, within b2876at 3013.010 kb on + strandat 3013.010 kb on + strandat 3013.012 kb on - strandat 3013.012 kb on - strandat 3013.026 kb on + strandat 3013.047 kb on + strandat 3013.047 kb on + strandat 3013.052 kb on + strandat 3013.052 kb on + strandat 3013.084 kb on + strandat 3013.121 kb on + strandat 3013.124 kb on - strandat 3013.125 kb on + strandat 3013.138 kb on + strandat 3013.139 kb on + strandat 3013.156 kb on - strandat 3013.166 kb on + strandat 3013.169 kb on - strandat 3013.177 kb on + strandat 3013.177 kb on + strandat 3013.184 kb on + strandat 3013.196 kb on - strandat 3013.198 kb on + strandat 3013.203 kb on - strandat 3013.228 kb on - strandat 3013.228 kb on - strandat 3013.236 kb on - strandat 3013.238 kb on + strandat 3013.241 kb on + strand, within ygfJat 3013.242 kb on + strand, within ygfJat 3013.248 kb on - strand, within ygfJat 3013.253 kb on + strand, within ygfJat 3013.253 kb on + strand, within ygfJat 3013.254 kb on + strand, within ygfJat 3013.254 kb on + strand, within ygfJat 3013.254 kb on + strand, within ygfJat 3013.256 kb on - strand, within ygfJ

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Per-strain Table

Position Strand Gene LocusTag Fraction R2_N4_T1_phage10
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3,009,654 + yqeA b2874 0.18 +2.1
3,009,688 + yqeA b2874 0.22 -2.7
3,009,701 - yqeA b2874 0.23 -0.3
3,009,701 - yqeA b2874 0.23 -2.7
3,009,730 + yqeA b2874 0.26 +0.9
3,009,816 - yqeA b2874 0.36 +0.7
3,009,822 - yqeA b2874 0.36 +0.8
3,009,922 - yqeA b2874 0.47 +0.0
3,010,002 + yqeA b2874 0.56 -0.5
3,010,013 + yqeA b2874 0.57 +1.6
3,010,232 + yqeA b2874 0.80 +0.3
3,010,232 + yqeA b2874 0.80 +0.4
3,010,280 + yqeA b2874 0.85 +0.0
3,010,282 + yqeA b2874 0.86 +2.3
3,010,282 + yqeA b2874 0.86 +0.2
3,010,349 + -1.3
3,010,390 + -0.1
3,010,590 + -1.3
3,010,596 - -2.4
3,010,610 + +2.0
3,010,618 - +0.9
3,010,618 - +0.1
3,010,697 - +0.3
3,010,803 + yqeB b2875 0.10 -1.2
3,010,822 - yqeB b2875 0.11 -1.8
3,010,822 - yqeB b2875 0.11 +0.3
3,010,914 - yqeB b2875 0.17 -0.2
3,010,941 + yqeB b2875 0.19 -0.9
3,010,952 - yqeB b2875 0.19 +1.8
3,010,964 - yqeB b2875 0.20 +0.3
3,010,980 - yqeB b2875 0.21 -1.1
3,011,204 - yqeB b2875 0.35 -0.4
3,011,209 + yqeB b2875 0.35 +0.2
3,011,418 - yqeB b2875 0.48 +0.2
3,011,469 + yqeB b2875 0.51 -2.9
3,011,537 + yqeB b2875 0.55 -1.0
3,011,560 + yqeB b2875 0.57 -0.7
3,011,597 - yqeB b2875 0.59 -0.4
3,011,616 - yqeB b2875 0.60 +0.8
3,011,706 - yqeB b2875 0.66 +0.8
3,011,918 - yqeB b2875 0.79 -1.7
3,011,918 - yqeB b2875 0.79 +0.2
3,011,971 + yqeB b2875 0.82 -0.6
3,012,000 - yqeB b2875 0.84 -0.1
3,012,020 - -0.5
3,012,020 - yqeB b2875 0.85 -0.5
3,012,022 + yqeB b2875 0.85 -3.3
3,012,079 - yqeB b2875 0.89 +1.1
3,012,079 - yqeB b2875 0.89 +1.0
3,012,102 - -0.3
3,012,147 - -0.7
3,012,283 - -0.9
3,012,304 + -0.2
3,012,378 + -0.6
3,012,497 - b2876 b2876 0.24 +1.0
3,012,666 - b2876 b2876 0.46 +0.2
3,012,666 - b2876 b2876 0.46 -1.7
3,012,715 + b2876 b2876 0.53 -0.1
3,012,848 + b2876 b2876 0.70 +0.1
3,012,888 + b2876 b2876 0.75 +1.0
3,012,888 + b2876 b2876 0.75 +4.3
3,012,939 + b2876 b2876 0.82 -1.2
3,012,947 - b2876 b2876 0.83 -1.6
3,012,954 + b2876 b2876 0.84 +1.0
3,013,010 + +0.9
3,013,010 + +0.7
3,013,012 - -1.2
3,013,012 - -1.6
3,013,026 + +0.2
3,013,047 + -0.5
3,013,047 + +1.3
3,013,052 + -0.0
3,013,052 + +1.7
3,013,084 + -0.1
3,013,121 + -1.0
3,013,124 - +0.4
3,013,125 + +0.4
3,013,138 + +1.7
3,013,139 + +0.7
3,013,156 - +0.4
3,013,166 + +1.4
3,013,169 - -1.6
3,013,177 + -0.6
3,013,177 + -0.3
3,013,184 + -1.7
3,013,196 - +0.4
3,013,198 + -1.9
3,013,203 - -2.2
3,013,228 - -1.6
3,013,228 - -2.2
3,013,236 - -0.6
3,013,238 + -3.5
3,013,241 + ygfJ b2877 0.10 +0.1
3,013,242 + ygfJ b2877 0.10 +1.0
3,013,248 - ygfJ b2877 0.11 +0.4
3,013,253 + ygfJ b2877 0.12 -0.8
3,013,253 + ygfJ b2877 0.12 -0.3
3,013,254 + ygfJ b2877 0.12 -0.4
3,013,254 + ygfJ b2877 0.12 -0.3
3,013,254 + ygfJ b2877 0.12 -0.8
3,013,256 - ygfJ b2877 0.13 -1.9

Or see this region's nucleotide sequence