Experiment: R2_N4_T1_phage10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheB and cheR are separated by 2 nucleotides cheR and tap are separated by 18 nucleotides tap and tar are separated by 45 nucleotides
b1883: cheB - chemotaxis-specific methylesterase (NCBI), at 1,965,476 to 1,966,525
cheB
b1884: cheR - chemotaxis regulator, protein-glutamate methyltransferase (NCBI), at 1,966,528 to 1,967,388
cheR
b1885: tap - methyl-accepting protein IV (NCBI), at 1,967,407 to 1,969,008
tap
b1886: tar - methyl-accepting chemotaxis protein II (NCBI), at 1,969,054 to 1,970,715
tar
Position (kb)
1967
1968
1969
1970 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1966.514 kb on + strand at 1966.553 kb on - strand at 1966.628 kb on + strand, within cheR at 1966.628 kb on + strand, within cheR at 1966.690 kb on - strand, within cheR at 1966.733 kb on + strand, within cheR at 1966.883 kb on - strand, within cheR at 1966.883 kb on - strand, within cheR at 1966.890 kb on - strand, within cheR at 1967.084 kb on - strand, within cheR at 1967.202 kb on + strand, within cheR at 1967.413 kb on - strand at 1967.413 kb on - strand at 1967.576 kb on - strand, within tap at 1967.731 kb on + strand, within tap at 1967.997 kb on - strand, within tap at 1968.203 kb on + strand, within tap at 1968.218 kb on + strand, within tap at 1968.238 kb on - strand, within tap at 1968.316 kb on + strand, within tap at 1968.329 kb on - strand, within tap at 1968.571 kb on - strand, within tap at 1968.651 kb on + strand, within tap at 1968.651 kb on + strand, within tap at 1968.660 kb on + strand, within tap at 1968.786 kb on - strand, within tap at 1968.786 kb on - strand, within tap at 1968.828 kb on - strand, within tap at 1968.852 kb on + strand at 1969.099 kb on + strand at 1969.099 kb on + strand at 1969.425 kb on - strand, within tar at 1969.705 kb on + strand, within tar at 1969.705 kb on + strand, within tar at 1969.713 kb on - strand, within tar at 1969.833 kb on + strand, within tar at 1969.925 kb on - strand, within tar at 1969.957 kb on + strand, within tar
Per-strain Table
Position Strand Gene LocusTag Fraction R2_N4_T1_phage10 remove 1,966,514 + +1.2 1,966,553 - -2.2 1,966,628 + cheR b1884 0.12 -0.6 1,966,628 + cheR b1884 0.12 -0.1 1,966,690 - cheR b1884 0.19 -4.4 1,966,733 + cheR b1884 0.24 +1.0 1,966,883 - cheR b1884 0.41 +1.1 1,966,883 - cheR b1884 0.41 -1.3 1,966,890 - cheR b1884 0.42 +0.9 1,967,084 - cheR b1884 0.65 -0.4 1,967,202 + cheR b1884 0.78 +1.2 1,967,413 - -3.4 1,967,413 - -0.2 1,967,576 - tap b1885 0.11 -2.9 1,967,731 + tap b1885 0.20 +0.5 1,967,997 - tap b1885 0.37 +0.8 1,968,203 + tap b1885 0.50 -0.6 1,968,218 + tap b1885 0.51 -0.3 1,968,238 - tap b1885 0.52 -0.6 1,968,316 + tap b1885 0.57 -0.3 1,968,329 - tap b1885 0.58 +0.3 1,968,571 - tap b1885 0.73 +0.4 1,968,651 + tap b1885 0.78 +0.4 1,968,651 + tap b1885 0.78 -1.5 1,968,660 + tap b1885 0.78 -2.9 1,968,786 - tap b1885 0.86 +0.2 1,968,786 - tap b1885 0.86 +0.2 1,968,828 - tap b1885 0.89 +0.1 1,968,852 + -0.6 1,969,099 + -0.1 1,969,099 + +0.0 1,969,425 - tar b1886 0.22 +0.3 1,969,705 + tar b1886 0.39 -0.7 1,969,705 + tar b1886 0.39 -0.5 1,969,713 - tar b1886 0.40 +0.0 1,969,833 + tar b1886 0.47 +0.3 1,969,925 - tar b1886 0.52 +0.4 1,969,957 + tar b1886 0.54 +0.5
Or see this region's nucleotide sequence