Strain Fitness in Escherichia coli BW25113 around b3486
Experiment: R1_N4_T2_phage8
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | R1_N4_T2_phage8 |
---|---|---|---|---|---|
remove | |||||
3,623,841 | + | yhhJ | b3485 | 0.12 | -0.4 |
3,623,864 | + | yhhJ | b3485 | 0.14 | -3.3 |
3,623,869 | + | yhhJ | b3485 | 0.15 | +2.8 |
3,623,874 | + | yhhJ | b3485 | 0.15 | +0.9 |
3,623,874 | + | yhhJ | b3485 | 0.15 | -3.3 |
3,623,876 | + | yhhJ | b3485 | 0.15 | +0.2 |
3,623,906 | - | yhhJ | b3485 | 0.18 | -0.0 |
3,623,916 | - | yhhJ | b3485 | 0.19 | -3.0 |
3,623,916 | - | yhhJ | b3485 | 0.19 | -3.5 |
3,623,969 | - | yhhJ | b3485 | 0.24 | -2.4 |
3,624,083 | + | yhhJ | b3485 | 0.34 | -3.4 |
3,624,085 | + | yhhJ | b3485 | 0.34 | -1.7 |
3,624,112 | + | yhhJ | b3485 | 0.36 | +0.1 |
3,624,130 | + | yhhJ | b3485 | 0.38 | -1.8 |
3,624,200 | + | yhhJ | b3485 | 0.44 | +0.4 |
3,624,215 | + | yhhJ | b3485 | 0.46 | -0.1 |
3,624,235 | + | yhhJ | b3485 | 0.47 | +1.1 |
3,624,238 | - | yhhJ | b3485 | 0.48 | +0.6 |
3,624,238 | - | yhhJ | b3485 | 0.48 | -2.9 |
3,624,238 | - | yhhJ | b3485 | 0.48 | +1.4 |
3,624,256 | - | yhhJ | b3485 | 0.49 | -3.5 |
3,624,256 | - | yhhJ | b3485 | 0.49 | +0.4 |
3,624,273 | - | yhhJ | b3485 | 0.51 | +0.2 |
3,624,317 | - | yhhJ | b3485 | 0.55 | -1.3 |
3,624,317 | - | yhhJ | b3485 | 0.55 | -4.3 |
3,624,365 | + | yhhJ | b3485 | 0.59 | -1.0 |
3,624,407 | + | yhhJ | b3485 | 0.63 | -0.9 |
3,624,407 | + | yhhJ | b3485 | 0.63 | +1.2 |
3,624,448 | - | yhhJ | b3485 | 0.66 | -0.9 |
3,624,489 | + | yhhJ | b3485 | 0.70 | +1.2 |
3,624,539 | + | yhhJ | b3485 | 0.74 | -3.0 |
3,624,566 | - | yhhJ | b3485 | 0.77 | +2.9 |
3,624,566 | - | yhhJ | b3485 | 0.77 | -2.5 |
3,624,612 | + | yhhJ | b3485 | 0.81 | -3.3 |
3,624,612 | + | yhhJ | b3485 | 0.81 | +2.0 |
3,624,683 | + | yhhJ | b3485 | 0.87 | +0.1 |
3,624,779 | + | -0.1 | |||
3,624,878 | - | -4.5 | |||
3,624,878 | - | +1.5 | |||
3,624,968 | - | -3.5 | |||
3,624,968 | - | -0.7 | |||
3,624,996 | + | -1.1 | |||
3,624,997 | - | +0.3 | |||
3,625,130 | - | rbbA | b3486 | 0.11 | +1.2 |
3,625,142 | + | rbbA | b3486 | 0.12 | +1.9 |
3,625,249 | - | rbbA | b3486 | 0.15 | +2.1 |
3,625,264 | - | rbbA | b3486 | 0.16 | +0.3 |
3,625,264 | - | rbbA | b3486 | 0.16 | +1.7 |
3,625,304 | - | rbbA | b3486 | 0.17 | +0.0 |
3,625,304 | - | rbbA | b3486 | 0.17 | +0.8 |
3,625,319 | + | rbbA | b3486 | 0.18 | -0.1 |
3,625,420 | - | rbbA | b3486 | 0.22 | +0.7 |
3,625,420 | - | rbbA | b3486 | 0.22 | +0.5 |
3,625,517 | + | rbbA | b3486 | 0.25 | +1.7 |
3,625,517 | + | rbbA | b3486 | 0.25 | +0.5 |
3,625,520 | - | rbbA | b3486 | 0.25 | +0.1 |
3,625,525 | + | rbbA | b3486 | 0.26 | +0.8 |
3,625,525 | - | rbbA | b3486 | 0.26 | +0.8 |
3,625,528 | + | rbbA | b3486 | 0.26 | -3.7 |
3,625,530 | - | rbbA | b3486 | 0.26 | +0.8 |
3,625,530 | - | rbbA | b3486 | 0.26 | -5.0 |
3,625,533 | - | rbbA | b3486 | 0.26 | -3.0 |
3,625,533 | - | rbbA | b3486 | 0.26 | +0.6 |
3,625,567 | + | rbbA | b3486 | 0.27 | +0.3 |
3,625,678 | + | rbbA | b3486 | 0.31 | -0.7 |
3,625,736 | + | rbbA | b3486 | 0.33 | -0.7 |
3,625,736 | + | rbbA | b3486 | 0.33 | +1.5 |
3,625,736 | + | rbbA | b3486 | 0.33 | +0.3 |
3,625,754 | + | rbbA | b3486 | 0.34 | +1.5 |
3,625,779 | + | rbbA | b3486 | 0.35 | -2.0 |
3,625,779 | + | rbbA | b3486 | 0.35 | +1.7 |
3,625,794 | - | rbbA | b3486 | 0.35 | -3.7 |
3,625,794 | - | rbbA | b3486 | 0.35 | +2.5 |
3,625,802 | - | rbbA | b3486 | 0.36 | +0.1 |
3,625,864 | + | rbbA | b3486 | 0.38 | +0.7 |
3,625,864 | + | rbbA | b3486 | 0.38 | -0.4 |
3,625,910 | - | rbbA | b3486 | 0.40 | -1.3 |
3,625,946 | - | rbbA | b3486 | 0.41 | -0.6 |
3,625,946 | - | rbbA | b3486 | 0.41 | -0.3 |
3,625,983 | - | rbbA | b3486 | 0.42 | +0.0 |
3,625,983 | - | rbbA | b3486 | 0.42 | -0.4 |
3,626,003 | + | rbbA | b3486 | 0.43 | -2.9 |
3,626,098 | - | rbbA | b3486 | 0.46 | +0.7 |
3,626,098 | - | rbbA | b3486 | 0.46 | +1.8 |
3,626,099 | - | rbbA | b3486 | 0.47 | -0.5 |
3,626,099 | - | rbbA | b3486 | 0.47 | -0.0 |
3,626,119 | - | rbbA | b3486 | 0.47 | -4.1 |
3,626,119 | - | rbbA | b3486 | 0.47 | -2.4 |
3,626,121 | + | rbbA | b3486 | 0.47 | +0.3 |
3,626,156 | + | rbbA | b3486 | 0.49 | +0.1 |
3,626,191 | + | rbbA | b3486 | 0.50 | +1.5 |
3,626,238 | + | rbbA | b3486 | 0.52 | +0.8 |
3,626,306 | - | rbbA | b3486 | 0.54 | +1.3 |
3,626,368 | + | rbbA | b3486 | 0.56 | -0.1 |
3,626,368 | + | rbbA | b3486 | 0.56 | -3.5 |
3,626,398 | - | rbbA | b3486 | 0.57 | -3.9 |
3,626,398 | - | rbbA | b3486 | 0.57 | +2.3 |
3,626,458 | + | rbbA | b3486 | 0.60 | -0.3 |
3,626,511 | + | rbbA | b3486 | 0.62 | +0.4 |
3,626,581 | + | rbbA | b3486 | 0.64 | +1.6 |
3,626,617 | - | rbbA | b3486 | 0.65 | -0.1 |
3,626,617 | - | rbbA | b3486 | 0.65 | -0.7 |
3,626,720 | - | rbbA | b3486 | 0.69 | -4.8 |
3,626,767 | + | rbbA | b3486 | 0.71 | +0.4 |
3,626,771 | + | rbbA | b3486 | 0.71 | +0.2 |
3,626,771 | + | rbbA | b3486 | 0.71 | +0.7 |
3,626,775 | - | rbbA | b3486 | 0.71 | +1.7 |
3,626,784 | - | rbbA | b3486 | 0.72 | +0.3 |
3,626,889 | + | rbbA | b3486 | 0.75 | +0.3 |
3,626,899 | + | rbbA | b3486 | 0.76 | -1.4 |
3,626,899 | + | rbbA | b3486 | 0.76 | -0.3 |
3,626,958 | + | rbbA | b3486 | 0.78 | -0.8 |
3,626,979 | + | rbbA | b3486 | 0.79 | -0.3 |
3,627,030 | + | rbbA | b3486 | 0.81 | +0.1 |
3,627,060 | + | rbbA | b3486 | 0.82 | +1.3 |
3,627,060 | + | rbbA | b3486 | 0.82 | -1.2 |
3,627,072 | + | rbbA | b3486 | 0.82 | -0.2 |
3,627,081 | - | rbbA | b3486 | 0.82 | +1.7 |
3,627,081 | - | rbbA | b3486 | 0.82 | -1.3 |
3,627,090 | + | rbbA | b3486 | 0.83 | +0.2 |
3,627,090 | + | rbbA | b3486 | 0.83 | -2.8 |
3,627,126 | - | rbbA | b3486 | 0.84 | +1.3 |
3,627,166 | - | rbbA | b3486 | 0.86 | +0.0 |
3,627,359 | - | +1.4 | |||
3,627,366 | + | -0.6 | |||
3,627,448 | - | +0.4 | |||
3,627,448 | - | +1.4 | |||
3,627,448 | - | +2.0 | |||
3,627,449 | - | -0.2 | |||
3,627,463 | - | -1.4 | |||
3,627,581 | - | -0.7 | |||
3,627,611 | + | +0.9 | |||
3,627,611 | + | -4.0 | |||
3,627,617 | + | +0.2 | |||
3,627,617 | + | +0.5 | |||
3,627,617 | + | -3.4 | |||
3,627,660 | + | +1.3 | |||
3,627,714 | - | yhiI | b3487 | 0.15 | +1.6 |
3,627,750 | - | yhiI | b3487 | 0.18 | -1.2 |
3,627,836 | + | yhiI | b3487 | 0.26 | +1.3 |
3,627,854 | - | yhiI | b3487 | 0.28 | -1.8 |
3,627,903 | + | yhiI | b3487 | 0.32 | -0.2 |
3,627,906 | - | yhiI | b3487 | 0.33 | -1.7 |
3,627,906 | - | yhiI | b3487 | 0.33 | -0.9 |
3,627,938 | - | yhiI | b3487 | 0.36 | +0.3 |
3,628,055 | - | yhiI | b3487 | 0.47 | -0.4 |
3,628,063 | + | yhiI | b3487 | 0.47 | -1.6 |
3,628,066 | - | yhiI | b3487 | 0.48 | +0.2 |
3,628,199 | + | yhiI | b3487 | 0.60 | -0.4 |
3,628,223 | + | yhiI | b3487 | 0.62 | +1.0 |
3,628,223 | + | yhiI | b3487 | 0.62 | +1.1 |
3,628,236 | + | yhiI | b3487 | 0.63 | +0.7 |
3,628,244 | - | yhiI | b3487 | 0.64 | -0.0 |
3,628,269 | - | yhiI | b3487 | 0.67 | +0.5 |
3,628,303 | + | yhiI | b3487 | 0.70 | -1.2 |
3,628,307 | - | yhiI | b3487 | 0.70 | +0.5 |
3,628,307 | - | yhiI | b3487 | 0.70 | +0.3 |
3,628,347 | - | yhiI | b3487 | 0.74 | -1.6 |
3,628,365 | - | yhiI | b3487 | 0.76 | +0.1 |
3,628,426 | - | yhiI | b3487 | 0.81 | +0.0 |
3,628,426 | - | yhiI | b3487 | 0.81 | +0.6 |
3,628,506 | + | yhiI | b3487 | 0.89 | -0.4 |
Or see this region's nucleotide sequence