Experiment: R1_N4_T2_phage8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pldB and yigL are separated by 7 nucleotides yigL and yigM are separated by 75 nucleotides yigM and metR overlap by 113 nucleotides
b3825: pldB - lysophospholipase L(2) (NCBI), at 4,007,193 to 4,008,215
pldB
b3826: yigL - predicted hydrolase (NCBI), at 4,008,223 to 4,009,023
yigL
b3827: yigM - predicted inner membrane protein (NCBI), at 4,009,099 to 4,009,998
yigM
b3828: metR - DNA-binding transcriptional activator, homocysteine-binding (NCBI), at 4,009,886 to 4,010,839
metR
Position (kb)
4009
4010 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 4008.225 kb on + strand at 4008.230 kb on + strand at 4008.230 kb on + strand at 4008.302 kb on + strand at 4008.302 kb on + strand at 4008.310 kb on - strand, within yigL at 4008.350 kb on + strand, within yigL at 4008.350 kb on + strand, within yigL at 4008.393 kb on - strand, within yigL at 4008.416 kb on + strand, within yigL at 4008.430 kb on + strand, within yigL at 4008.489 kb on - strand, within yigL at 4008.495 kb on + strand, within yigL at 4008.496 kb on - strand, within yigL at 4008.505 kb on + strand, within yigL at 4008.526 kb on - strand, within yigL at 4008.526 kb on - strand, within yigL at 4008.529 kb on + strand, within yigL at 4008.572 kb on + strand, within yigL at 4008.572 kb on + strand, within yigL at 4008.621 kb on + strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.632 kb on + strand, within yigL at 4008.677 kb on + strand, within yigL at 4008.677 kb on + strand, within yigL at 4008.680 kb on + strand, within yigL at 4008.688 kb on - strand, within yigL at 4008.708 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.762 kb on - strand, within yigL at 4008.762 kb on - strand, within yigL at 4008.903 kb on - strand, within yigL at 4008.910 kb on + strand, within yigL at 4008.918 kb on - strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.968 kb on + strand at 4009.067 kb on - strand at 4009.082 kb on - strand at 4009.134 kb on - strand at 4009.176 kb on - strand at 4009.176 kb on - strand at 4009.205 kb on - strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.283 kb on - strand, within yigM at 4009.283 kb on - strand, within yigM at 4009.311 kb on - strand, within yigM at 4009.334 kb on + strand, within yigM at 4009.334 kb on + strand, within yigM at 4009.338 kb on - strand, within yigM at 4009.348 kb on - strand, within yigM at 4009.348 kb on - strand, within yigM at 4009.406 kb on - strand, within yigM at 4009.428 kb on + strand, within yigM at 4009.434 kb on + strand, within yigM at 4009.434 kb on + strand, within yigM at 4009.451 kb on - strand, within yigM at 4009.495 kb on - strand, within yigM at 4009.495 kb on - strand, within yigM at 4009.506 kb on + strand, within yigM at 4009.524 kb on - strand, within yigM at 4009.600 kb on + strand, within yigM at 4009.772 kb on + strand, within yigM at 4009.839 kb on - strand, within yigM at 4009.839 kb on - strand, within yigM at 4009.845 kb on - strand, within yigM at 4009.845 kb on - strand, within yigM at 4009.874 kb on + strand, within yigM at 4009.904 kb on + strand at 4009.917 kb on - strand at 4009.921 kb on - strand at 4009.921 kb on - strand at 4009.947 kb on - strand at 4010.026 kb on - strand, within metR at 4010.026 kb on - strand, within metR at 4010.038 kb on - strand, within metR at 4010.048 kb on - strand, within metR at 4010.094 kb on - strand, within metR at 4010.094 kb on - strand, within metR at 4010.094 kb on - strand, within metR at 4010.100 kb on - strand, within metR at 4010.113 kb on + strand, within metR at 4010.152 kb on - strand, within metR at 4010.245 kb on + strand, within metR at 4010.247 kb on + strand, within metR at 4010.267 kb on + strand, within metR at 4010.267 kb on + strand, within metR at 4010.337 kb on - strand, within metR at 4010.353 kb on + strand, within metR at 4010.386 kb on - strand, within metR at 4010.386 kb on - strand, within metR at 4010.386 kb on - strand, within metR at 4010.461 kb on - strand, within metR at 4010.461 kb on - strand, within metR at 4010.541 kb on + strand, within metR at 4010.541 kb on + strand, within metR at 4010.546 kb on - strand, within metR at 4010.549 kb on - strand, within metR at 4010.560 kb on - strand, within metR at 4010.560 kb on - strand, within metR at 4010.572 kb on - strand, within metR at 4010.572 kb on - strand, within metR at 4010.677 kb on - strand, within metR at 4010.729 kb on + strand, within metR at 4010.969 kb on + strand at 4010.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R1_N4_T2_phage8 remove 4,008,225 + -0.2 4,008,230 + -0.3 4,008,230 + -3.8 4,008,302 + +0.3 4,008,302 + -0.8 4,008,310 - yigL b3826 0.11 +1.0 4,008,350 + yigL b3826 0.16 +0.6 4,008,350 + yigL b3826 0.16 -0.2 4,008,393 - yigL b3826 0.21 +0.3 4,008,416 + yigL b3826 0.24 -0.8 4,008,430 + yigL b3826 0.26 -1.5 4,008,489 - yigL b3826 0.33 +2.0 4,008,495 + yigL b3826 0.34 +0.6 4,008,496 - yigL b3826 0.34 -3.7 4,008,505 + yigL b3826 0.35 +0.3 4,008,526 - yigL b3826 0.38 -0.9 4,008,526 - yigL b3826 0.38 -1.6 4,008,529 + yigL b3826 0.38 -0.0 4,008,572 + yigL b3826 0.44 +2.2 4,008,572 + yigL b3826 0.44 +1.6 4,008,621 + yigL b3826 0.50 +1.6 4,008,629 - yigL b3826 0.51 -0.5 4,008,629 - yigL b3826 0.51 +2.5 4,008,629 - yigL b3826 0.51 -0.3 4,008,632 + yigL b3826 0.51 -2.3 4,008,677 + yigL b3826 0.57 -0.6 4,008,677 + yigL b3826 0.57 +0.7 4,008,680 + yigL b3826 0.57 +3.0 4,008,688 - yigL b3826 0.58 -0.4 4,008,708 + yigL b3826 0.61 +0.2 4,008,754 + yigL b3826 0.66 -3.6 4,008,754 + yigL b3826 0.66 +0.8 4,008,754 + yigL b3826 0.66 +0.8 4,008,754 + yigL b3826 0.66 -3.7 4,008,762 - yigL b3826 0.67 -3.5 4,008,762 - yigL b3826 0.67 -2.1 4,008,903 - yigL b3826 0.85 +2.9 4,008,910 + yigL b3826 0.86 +1.3 4,008,918 - yigL b3826 0.87 +1.7 4,008,920 + yigL b3826 0.87 -0.3 4,008,920 + yigL b3826 0.87 +0.8 4,008,920 + yigL b3826 0.87 +0.4 4,008,968 + -2.2 4,009,067 - +1.0 4,009,082 - +0.2 4,009,134 - +0.2 4,009,176 - +0.5 4,009,176 - +0.8 4,009,205 - yigM b3827 0.12 +0.8 4,009,275 + yigM b3827 0.20 +0.2 4,009,275 + yigM b3827 0.20 -3.7 4,009,275 + yigM b3827 0.20 -2.3 4,009,275 + yigM b3827 0.20 -0.8 4,009,275 + yigM b3827 0.20 -0.4 4,009,275 + yigM b3827 0.20 +0.7 4,009,283 - yigM b3827 0.20 +0.0 4,009,283 - yigM b3827 0.20 +0.1 4,009,311 - yigM b3827 0.24 -0.4 4,009,334 + yigM b3827 0.26 -2.1 4,009,334 + yigM b3827 0.26 -0.4 4,009,338 - yigM b3827 0.27 -1.6 4,009,348 - yigM b3827 0.28 -0.3 4,009,348 - yigM b3827 0.28 -1.9 4,009,406 - yigM b3827 0.34 -2.2 4,009,428 + yigM b3827 0.37 +0.5 4,009,434 + yigM b3827 0.37 -4.3 4,009,434 + yigM b3827 0.37 +0.5 4,009,451 - yigM b3827 0.39 -1.1 4,009,495 - yigM b3827 0.44 -1.5 4,009,495 - yigM b3827 0.44 +1.3 4,009,506 + yigM b3827 0.45 -1.2 4,009,524 - yigM b3827 0.47 -0.3 4,009,600 + yigM b3827 0.56 +1.6 4,009,772 + yigM b3827 0.75 -1.6 4,009,839 - yigM b3827 0.82 -1.0 4,009,839 - yigM b3827 0.82 -0.6 4,009,845 - yigM b3827 0.83 +0.2 4,009,845 - yigM b3827 0.83 -3.1 4,009,874 + yigM b3827 0.86 -3.9 4,009,904 + -0.1 4,009,917 - +1.4 4,009,921 - -2.9 4,009,921 - -3.3 4,009,947 - +0.0 4,010,026 - metR b3828 0.15 -1.9 4,010,026 - metR b3828 0.15 +1.4 4,010,038 - metR b3828 0.16 +0.0 4,010,048 - metR b3828 0.17 +1.1 4,010,094 - metR b3828 0.22 +1.6 4,010,094 - metR b3828 0.22 -1.4 4,010,094 - metR b3828 0.22 +0.4 4,010,100 - metR b3828 0.22 +0.9 4,010,113 + metR b3828 0.24 -0.8 4,010,152 - metR b3828 0.28 +0.7 4,010,245 + metR b3828 0.38 +0.3 4,010,247 + metR b3828 0.38 -1.0 4,010,267 + metR b3828 0.40 +1.8 4,010,267 + metR b3828 0.40 -4.8 4,010,337 - metR b3828 0.47 -0.1 4,010,353 + metR b3828 0.49 +1.2 4,010,386 - metR b3828 0.52 -0.2 4,010,386 - metR b3828 0.52 -0.8 4,010,386 - metR b3828 0.52 +0.8 4,010,461 - metR b3828 0.60 +0.3 4,010,461 - metR b3828 0.60 -3.4 4,010,541 + metR b3828 0.69 -2.3 4,010,541 + metR b3828 0.69 -0.5 4,010,546 - metR b3828 0.69 -0.2 4,010,549 - metR b3828 0.69 +2.3 4,010,560 - metR b3828 0.71 +0.8 4,010,560 - metR b3828 0.71 +0.5 4,010,572 - metR b3828 0.72 -1.2 4,010,572 - metR b3828 0.72 -0.1 4,010,677 - metR b3828 0.83 -0.1 4,010,729 + metR b3828 0.88 -0.6 4,010,969 + -0.6 4,010,977 - +0.6
Or see this region's nucleotide sequence