Experiment: R1_N4_T2_phage8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaL and yeaM overlap by 44 nucleotides yeaM and yeaN are separated by 96 nucleotides yeaN and yeaO are separated by 54 nucleotides yeaO and yoaF are separated by 21 nucleotides yoaF and yeaP are separated by 182 nucleotides
b1789: yeaL - conserved inner membrane protein (NCBI), at 1,872,376 to 1,872,822
yeaL
b1790: yeaM - predicted DNA-binding transcriptional regulator (NCBI), at 1,872,779 to 1,873,600
yeaM
b1791: yeaN - predicted transporter (NCBI), at 1,873,697 to 1,874,878
yeaN
b1792: yeaO - orf, hypothetical protein (VIMSS), at 1,874,933 to 1,875,280
yeaO
b1793: yoaF - conserved outer membrane protein (NCBI), at 1,875,302 to 1,875,556
yoaF
b1794: yeaP - orf, hypothetical protein (VIMSS), at 1,875,739 to 1,876,764
yeaP
Position (kb)
1873
1874
1875 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1873.148 kb on + strand, within yeaM at 1873.370 kb on + strand, within yeaM at 1873.391 kb on - strand, within yeaM at 1873.472 kb on + strand, within yeaM at 1873.472 kb on + strand, within yeaM at 1873.510 kb on + strand, within yeaM at 1873.559 kb on - strand at 1873.744 kb on - strand at 1873.791 kb on + strand at 1873.799 kb on - strand at 1873.839 kb on - strand, within yeaN at 1873.888 kb on + strand, within yeaN at 1873.888 kb on + strand, within yeaN at 1874.141 kb on - strand, within yeaN at 1874.429 kb on - strand, within yeaN at 1874.429 kb on - strand, within yeaN at 1874.429 kb on - strand, within yeaN at 1874.429 kb on - strand, within yeaN at 1874.457 kb on - strand, within yeaN at 1874.518 kb on - strand, within yeaN at 1874.614 kb on + strand, within yeaN at 1874.783 kb on + strand at 1875.211 kb on + strand, within yeaO at 1875.211 kb on + strand, within yeaO at 1875.211 kb on + strand, within yeaO at 1875.241 kb on - strand, within yeaO at 1875.241 kb on - strand, within yeaO at 1875.291 kb on + strand at 1875.416 kb on - strand, within yoaF at 1875.530 kb on + strand, within yoaF at 1875.571 kb on + strand at 1875.582 kb on + strand at 1875.724 kb on + strand at 1875.848 kb on - strand, within yeaP
Per-strain Table
Position Strand Gene LocusTag Fraction R1_N4_T2_phage8 remove 1,873,148 + yeaM b1790 0.45 -0.3 1,873,370 + yeaM b1790 0.72 -3.5 1,873,391 - yeaM b1790 0.74 +2.4 1,873,472 + yeaM b1790 0.84 -4.9 1,873,472 + yeaM b1790 0.84 -0.4 1,873,510 + yeaM b1790 0.89 -2.6 1,873,559 - +0.9 1,873,744 - +1.1 1,873,791 + -3.1 1,873,799 - -3.9 1,873,839 - yeaN b1791 0.12 -1.3 1,873,888 + yeaN b1791 0.16 -3.9 1,873,888 + yeaN b1791 0.16 -3.5 1,874,141 - yeaN b1791 0.38 +1.3 1,874,429 - yeaN b1791 0.62 -0.5 1,874,429 - yeaN b1791 0.62 -3.8 1,874,429 - yeaN b1791 0.62 -3.3 1,874,429 - yeaN b1791 0.62 -1.2 1,874,457 - yeaN b1791 0.64 -0.5 1,874,518 - yeaN b1791 0.69 +2.2 1,874,614 + yeaN b1791 0.78 -0.7 1,874,783 + -0.4 1,875,211 + yeaO b1792 0.80 -0.4 1,875,211 + yeaO b1792 0.80 +0.6 1,875,211 + yeaO b1792 0.80 -0.9 1,875,241 - yeaO b1792 0.89 -4.8 1,875,241 - yeaO b1792 0.89 +0.9 1,875,291 + +0.8 1,875,416 - yoaF b1793 0.45 +1.0 1,875,530 + yoaF b1793 0.89 -2.2 1,875,571 + +1.0 1,875,582 + -2.1 1,875,724 + -2.8 1,875,848 - yeaP b1794 0.11 -0.4
Or see this region's nucleotide sequence