Strain Fitness in Escherichia coli BW25113 around b0583

Experiment: R1_N4_T2_phage8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsL-2 and entD are separated by 281 nucleotidesentD and fepA are separated by 174 nucleotides b0582: insL-2 - IS186/IS421 transposase (NCBI), at 607,288 to 608,400 insL-2 b0583: entD - phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex (NCBI), at 608,682 to 609,302 entD b0584: fepA - iron-enterobactin outer membrane transporter (NCBI), at 609,477 to 611,717 fepA Position (kb) 608 609 610Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 608.621 kb on + strandat 608.621 kb on + strandat 608.621 kb on + strandat 608.629 kb on - strandat 608.669 kb on - strandat 608.750 kb on - strand, within entDat 608.761 kb on + strand, within entDat 608.766 kb on + strand, within entDat 608.834 kb on + strand, within entDat 609.183 kb on + strand, within entDat 609.246 kb on - strandat 609.246 kb on - strandat 609.253 kb on + strandat 609.253 kb on + strandat 609.266 kb on + strandat 609.266 kb on + strandat 609.277 kb on - strandat 609.277 kb on - strandat 609.284 kb on - strandat 609.303 kb on - strandat 609.304 kb on - strandat 609.338 kb on - strandat 609.338 kb on - strandat 609.498 kb on + strandat 609.536 kb on - strandat 609.549 kb on - strandat 609.580 kb on + strandat 609.597 kb on - strandat 609.597 kb on - strandat 609.724 kb on - strand, within fepAat 609.966 kb on - strand, within fepAat 610.025 kb on + strand, within fepAat 610.193 kb on - strand, within fepAat 610.193 kb on - strand, within fepAat 610.202 kb on - strand, within fepA

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Per-strain Table

Position Strand Gene LocusTag Fraction R1_N4_T2_phage8
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608,621 + -0.2
608,621 + +2.8
608,621 + -1.1
608,629 - +0.9
608,669 - +0.3
608,750 - entD b0583 0.11 +0.8
608,761 + entD b0583 0.13 +1.2
608,766 + entD b0583 0.14 -0.2
608,834 + entD b0583 0.24 +1.7
609,183 + entD b0583 0.81 +0.5
609,246 - +1.1
609,246 - +3.5
609,253 + +0.3
609,253 + -0.1
609,266 + +2.1
609,266 + +1.5
609,277 - +0.9
609,277 - -4.3
609,284 - +1.3
609,303 - +1.2
609,304 - +0.2
609,338 - +0.2
609,338 - +0.3
609,498 + -1.7
609,536 - +1.2
609,549 - -0.5
609,580 + +0.9
609,597 - +1.1
609,597 - -5.4
609,724 - fepA b0584 0.11 +0.9
609,966 - fepA b0584 0.22 +0.8
610,025 + fepA b0584 0.24 -1.0
610,193 - fepA b0584 0.32 -3.1
610,193 - fepA b0584 0.32 +0.1
610,202 - fepA b0584 0.32 +0.1

Or see this region's nucleotide sequence