Strain Fitness in Escherichia coli BW25113 around b4304

Experiment: R1_N4_T1_phage0

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsgcA and ryjB overlap by 4 nucleotidesryjB and sgcQ are separated by 44 nucleotidessgcQ and sgcC are separated by 12 nucleotidessgcC and sgcB are separated by 11 nucleotidessgcB and sgcX overlap by 4 nucleotides b4302: sgcA - KpLE2 phage-like element; predicted phosphotransferase enzyme IIA component (NCBI), at 4,525,572 to 4,526,003 sgcA b4624: ryjB - Novel sRNA, function unknown (RefSeq), at 4,526,000 to 4,526,089 ryjB b4303: sgcQ - KpLE2 phage-like element; predicted nucleoside triphosphatase (NCBI), at 4,526,134 to 4,526,940 sgcQ b4304: sgcC - KpLE2 phage-like element; predicted phosphotransferase enzyme IIC component (NCBI), at 4,526,953 to 4,528,266 sgcC b4565: sgcB - predicted enzyme IIB component of PTS (NCBI), at 4,528,278 to 4,528,556 sgcB b4305: sgcX - putative lyase/synthase (VIMSS), at 4,528,553 to 4,529,674 sgcX Position (kb) 4526 4527 4528 4529Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 4525.971 kb on - strandat 4526.051 kb on + strand, within ryjBat 4526.140 kb on + strandat 4526.140 kb on + strandat 4526.140 kb on + strandat 4526.164 kb on + strandat 4526.174 kb on + strandat 4526.174 kb on + strandat 4526.254 kb on + strand, within sgcQat 4526.254 kb on + strand, within sgcQat 4526.257 kb on + strand, within sgcQat 4526.298 kb on + strand, within sgcQat 4526.338 kb on - strandat 4526.356 kb on + strand, within sgcQat 4526.356 kb on + strand, within sgcQat 4526.388 kb on + strand, within sgcQat 4526.448 kb on + strand, within sgcQat 4526.448 kb on + strand, within sgcQat 4526.460 kb on + strand, within sgcQat 4526.461 kb on + strand, within sgcQat 4526.468 kb on - strand, within sgcQat 4526.468 kb on - strand, within sgcQat 4526.583 kb on - strand, within sgcQat 4526.621 kb on - strand, within sgcQat 4526.738 kb on + strand, within sgcQat 4526.769 kb on - strand, within sgcQat 4526.774 kb on + strand, within sgcQat 4526.809 kb on + strand, within sgcQat 4526.874 kb on + strandat 4526.885 kb on - strandat 4526.885 kb on - strandat 4526.895 kb on - strandat 4526.975 kb on + strandat 4526.975 kb on + strandat 4526.993 kb on - strandat 4526.993 kb on - strandat 4527.227 kb on - strand, within sgcCat 4527.239 kb on + strand, within sgcCat 4527.350 kb on - strand, within sgcCat 4527.357 kb on - strand, within sgcCat 4527.357 kb on - strand, within sgcCat 4527.373 kb on + strand, within sgcCat 4527.381 kb on + strand, within sgcCat 4527.381 kb on + strand, within sgcCat 4527.390 kb on + strand, within sgcCat 4527.400 kb on + strand, within sgcCat 4527.406 kb on - strand, within sgcCat 4527.441 kb on + strand, within sgcCat 4527.441 kb on + strand, within sgcCat 4527.504 kb on + strand, within sgcCat 4527.642 kb on + strand, within sgcCat 4527.648 kb on - strand, within sgcCat 4527.702 kb on + strand, within sgcCat 4527.702 kb on + strand, within sgcCat 4527.752 kb on - strand, within sgcCat 4527.850 kb on - strand, within sgcCat 4527.875 kb on + strand, within sgcCat 4527.875 kb on + strand, within sgcCat 4527.940 kb on + strand, within sgcCat 4528.011 kb on - strand, within sgcCat 4528.090 kb on - strand, within sgcCat 4528.092 kb on + strand, within sgcCat 4528.092 kb on + strand, within sgcCat 4528.120 kb on + strand, within sgcCat 4528.125 kb on + strand, within sgcCat 4528.152 kb on + strandat 4528.153 kb on + strandat 4528.153 kb on + strandat 4528.171 kb on - strandat 4528.185 kb on - strandat 4528.185 kb on - strandat 4528.187 kb on - strandat 4528.194 kb on + strandat 4528.202 kb on - strandat 4528.218 kb on + strandat 4528.221 kb on - strandat 4528.251 kb on + strandat 4528.264 kb on + strandat 4528.264 kb on + strandat 4528.292 kb on - strandat 4528.307 kb on + strand, within sgcBat 4528.307 kb on + strand, within sgcBat 4528.328 kb on + strand, within sgcBat 4528.328 kb on + strand, within sgcBat 4528.329 kb on - strand, within sgcBat 4528.330 kb on + strand, within sgcBat 4528.330 kb on + strand, within sgcBat 4528.330 kb on + strand, within sgcBat 4528.358 kb on - strand, within sgcBat 4528.393 kb on - strand, within sgcBat 4528.393 kb on - strand, within sgcBat 4528.411 kb on - strand, within sgcBat 4528.411 kb on - strand, within sgcBat 4528.412 kb on - strand, within sgcBat 4528.412 kb on - strand, within sgcBat 4528.424 kb on + strand, within sgcBat 4528.451 kb on - strand, within sgcBat 4528.573 kb on + strandat 4528.575 kb on + strandat 4528.575 kb on + strandat 4528.578 kb on - strandat 4528.578 kb on - strandat 4528.594 kb on + strandat 4528.698 kb on - strand, within sgcXat 4528.700 kb on + strand, within sgcXat 4528.707 kb on + strand, within sgcXat 4528.707 kb on + strand, within sgcXat 4528.848 kb on + strand, within sgcXat 4528.848 kb on + strand, within sgcXat 4528.953 kb on + strand, within sgcXat 4528.960 kb on - strand, within sgcXat 4528.966 kb on - strand, within sgcXat 4529.011 kb on - strand, within sgcXat 4529.039 kb on + strand, within sgcXat 4529.062 kb on + strand, within sgcXat 4529.080 kb on + strand, within sgcXat 4529.080 kb on + strand, within sgcXat 4529.080 kb on - strand, within sgcXat 4529.080 kb on - strand, within sgcXat 4529.088 kb on - strand, within sgcXat 4529.098 kb on - strand, within sgcXat 4529.121 kb on - strand, within sgcXat 4529.123 kb on + strand, within sgcXat 4529.161 kb on - strand, within sgcXat 4529.183 kb on + strand, within sgcXat 4529.189 kb on - strand, within sgcXat 4529.189 kb on - strand, within sgcXat 4529.189 kb on - strand, within sgcXat 4529.208 kb on + strand, within sgcXat 4529.208 kb on + strand, within sgcXat 4529.209 kb on + strand, within sgcXat 4529.221 kb on + strand, within sgcXat 4529.229 kb on - strand, within sgcX

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Per-strain Table

Position Strand Gene LocusTag Fraction R1_N4_T1_phage0
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4,525,971 - -0.2
4,526,051 + ryjB b4624 0.57 +1.9
4,526,140 + -2.3
4,526,140 + +0.2
4,526,140 + -3.8
4,526,164 + +0.7
4,526,174 + +1.0
4,526,174 + -3.8
4,526,254 + sgcQ b4303 0.15 +0.8
4,526,254 + sgcQ b4303 0.15 -3.9
4,526,257 + sgcQ b4303 0.15 +0.4
4,526,298 + sgcQ b4303 0.20 -1.1
4,526,338 - -1.1
4,526,356 + sgcQ b4303 0.28 -1.3
4,526,356 + sgcQ b4303 0.28 -4.0
4,526,388 + sgcQ b4303 0.31 +0.7
4,526,448 + sgcQ b4303 0.39 -1.6
4,526,448 + sgcQ b4303 0.39 +3.0
4,526,460 + sgcQ b4303 0.40 -0.9
4,526,461 + sgcQ b4303 0.41 +0.9
4,526,468 - sgcQ b4303 0.41 -0.4
4,526,468 - sgcQ b4303 0.41 -2.6
4,526,583 - sgcQ b4303 0.56 -4.2
4,526,621 - sgcQ b4303 0.60 -1.1
4,526,738 + sgcQ b4303 0.75 +0.0
4,526,769 - sgcQ b4303 0.79 -0.8
4,526,774 + sgcQ b4303 0.79 +1.8
4,526,809 + sgcQ b4303 0.84 -3.0
4,526,874 + -2.1
4,526,885 - -0.9
4,526,885 - -3.9
4,526,895 - +1.6
4,526,975 + -4.9
4,526,975 + -3.7
4,526,993 - -0.3
4,526,993 - -0.5
4,527,227 - sgcC b4304 0.21 +0.6
4,527,239 + sgcC b4304 0.22 -5.0
4,527,350 - sgcC b4304 0.30 +0.3
4,527,357 - sgcC b4304 0.31 -0.1
4,527,357 - sgcC b4304 0.31 +0.5
4,527,373 + sgcC b4304 0.32 +0.2
4,527,381 + sgcC b4304 0.33 -4.0
4,527,381 + sgcC b4304 0.33 -4.7
4,527,390 + sgcC b4304 0.33 -0.3
4,527,400 + sgcC b4304 0.34 +0.3
4,527,406 - sgcC b4304 0.34 -0.8
4,527,441 + sgcC b4304 0.37 +0.1
4,527,441 + sgcC b4304 0.37 +1.0
4,527,504 + sgcC b4304 0.42 +3.9
4,527,642 + sgcC b4304 0.52 +0.5
4,527,648 - sgcC b4304 0.53 +0.5
4,527,702 + sgcC b4304 0.57 +1.3
4,527,702 + sgcC b4304 0.57 -1.3
4,527,752 - sgcC b4304 0.61 +3.2
4,527,850 - sgcC b4304 0.68 +0.7
4,527,875 + sgcC b4304 0.70 +0.3
4,527,875 + sgcC b4304 0.70 -1.6
4,527,940 + sgcC b4304 0.75 +0.2
4,528,011 - sgcC b4304 0.81 +0.7
4,528,090 - sgcC b4304 0.87 -0.3
4,528,092 + sgcC b4304 0.87 -5.1
4,528,092 + sgcC b4304 0.87 -5.3
4,528,120 + sgcC b4304 0.89 +1.4
4,528,125 + sgcC b4304 0.89 -0.7
4,528,152 + -4.8
4,528,153 + +1.7
4,528,153 + -0.7
4,528,171 - +0.1
4,528,185 - +0.3
4,528,185 - -0.0
4,528,187 - +1.5
4,528,194 + -0.3
4,528,202 - +1.1
4,528,218 + -0.8
4,528,221 - +0.7
4,528,251 + +0.1
4,528,264 + +0.1
4,528,264 + +2.1
4,528,292 - -0.1
4,528,307 + sgcB b4565 0.10 -1.1
4,528,307 + sgcB b4565 0.10 -3.3
4,528,328 + sgcB b4565 0.18 -4.6
4,528,328 + sgcB b4565 0.18 -3.1
4,528,329 - sgcB b4565 0.18 +0.4
4,528,330 + sgcB b4565 0.19 -1.3
4,528,330 + sgcB b4565 0.19 +0.4
4,528,330 + sgcB b4565 0.19 +1.6
4,528,358 - sgcB b4565 0.29 -0.5
4,528,393 - sgcB b4565 0.41 -0.3
4,528,393 - sgcB b4565 0.41 -3.8
4,528,411 - sgcB b4565 0.48 -1.6
4,528,411 - sgcB b4565 0.48 +2.1
4,528,412 - sgcB b4565 0.48 -5.1
4,528,412 - sgcB b4565 0.48 -3.6
4,528,424 + sgcB b4565 0.52 -0.1
4,528,451 - sgcB b4565 0.62 -0.1
4,528,573 + +0.9
4,528,575 + -0.9
4,528,575 + -5.0
4,528,578 - -0.1
4,528,578 - +0.6
4,528,594 + -4.8
4,528,698 - sgcX b4305 0.13 +0.3
4,528,700 + sgcX b4305 0.13 +0.3
4,528,707 + sgcX b4305 0.14 -3.5
4,528,707 + sgcX b4305 0.14 +0.2
4,528,848 + sgcX b4305 0.26 +2.6
4,528,848 + sgcX b4305 0.26 -4.2
4,528,953 + sgcX b4305 0.36 -0.5
4,528,960 - sgcX b4305 0.36 +0.8
4,528,966 - sgcX b4305 0.37 +0.9
4,529,011 - sgcX b4305 0.41 +0.6
4,529,039 + sgcX b4305 0.43 +0.6
4,529,062 + sgcX b4305 0.45 +0.3
4,529,080 + sgcX b4305 0.47 -0.3
4,529,080 + sgcX b4305 0.47 +1.2
4,529,080 - sgcX b4305 0.47 +0.3
4,529,080 - sgcX b4305 0.47 +1.3
4,529,088 - sgcX b4305 0.48 -5.1
4,529,098 - sgcX b4305 0.49 -0.2
4,529,121 - sgcX b4305 0.51 -0.2
4,529,123 + sgcX b4305 0.51 +0.3
4,529,161 - sgcX b4305 0.54 +1.5
4,529,183 + sgcX b4305 0.56 +1.9
4,529,189 - sgcX b4305 0.57 -1.4
4,529,189 - sgcX b4305 0.57 -0.2
4,529,189 - sgcX b4305 0.57 -4.4
4,529,208 + sgcX b4305 0.58 -1.4
4,529,208 + sgcX b4305 0.58 -3.0
4,529,209 + sgcX b4305 0.58 +1.1
4,529,221 + sgcX b4305 0.60 +1.1
4,529,229 - sgcX b4305 0.60 -1.8

Or see this region's nucleotide sequence