Strain Fitness in Escherichia coli BW25113 around b2015

Experiment: R1_N4_T1_phage0

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeF and yoeI overlap by 11 nucleotidesyoeI and yeeY are separated by 214 nucleotidesyeeY and yeeZ are separated by 45 nucleotidesyeeZ and yoeB are separated by 82 nucleotides b2014: yeeF - putative amino acid/amine transport protein (VIMSS), at 2,083,728 to 2,085,086 yeeF b4678: yoeI - expressed protein (RefSeq), at 2,085,076 to 2,085,138 yoeI b2015: yeeY - putative transcriptional regulator LYSR-type (VIMSS), at 2,085,353 to 2,086,282 yeeY b2016: yeeZ - predicted epimerase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,086,328 to 2,087,152 yeeZ b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489 yoeB Position (kb) 2085 2086 2087Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 2084.421 kb on + strand, within yeeFat 2084.421 kb on + strand, within yeeFat 2084.450 kb on - strand, within yeeFat 2084.462 kb on - strand, within yeeFat 2084.569 kb on - strand, within yeeFat 2084.606 kb on - strand, within yeeFat 2084.606 kb on - strand, within yeeFat 2084.651 kb on + strand, within yeeFat 2084.651 kb on + strand, within yeeFat 2084.741 kb on + strand, within yeeFat 2084.790 kb on + strand, within yeeFat 2084.790 kb on + strand, within yeeFat 2084.803 kb on - strand, within yeeFat 2084.945 kb on - strand, within yeeFat 2084.945 kb on - strand, within yeeFat 2085.023 kb on + strandat 2085.023 kb on + strandat 2085.092 kb on + strand, within yoeIat 2085.736 kb on + strand, within yeeYat 2085.786 kb on + strand, within yeeYat 2085.813 kb on + strand, within yeeYat 2085.852 kb on - strand, within yeeYat 2085.860 kb on + strand, within yeeYat 2085.860 kb on + strand, within yeeYat 2085.866 kb on + strand, within yeeYat 2086.064 kb on - strand, within yeeYat 2086.173 kb on + strand, within yeeYat 2086.181 kb on - strand, within yeeYat 2086.181 kb on - strand, within yeeYat 2086.264 kb on - strandat 2086.298 kb on - strandat 2086.338 kb on + strandat 2086.338 kb on + strandat 2086.351 kb on + strandat 2086.424 kb on + strand, within yeeZat 2086.458 kb on - strand, within yeeZat 2086.458 kb on - strand, within yeeZat 2086.500 kb on + strand, within yeeZat 2086.556 kb on - strand, within yeeZat 2086.579 kb on + strand, within yeeZat 2086.660 kb on + strand, within yeeZat 2086.660 kb on + strand, within yeeZat 2086.669 kb on + strand, within yeeZat 2086.672 kb on - strand, within yeeZat 2086.672 kb on - strand, within yeeZat 2086.871 kb on - strand, within yeeZat 2086.947 kb on - strand, within yeeZat 2087.058 kb on + strand, within yeeZat 2087.122 kb on + strandat 2087.178 kb on - strandat 2087.178 kb on - strandat 2087.187 kb on - strandat 2087.197 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R1_N4_T1_phage0
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2,084,421 + yeeF b2014 0.51 -2.7
2,084,421 + yeeF b2014 0.51 -2.2
2,084,450 - yeeF b2014 0.53 -1.7
2,084,462 - yeeF b2014 0.54 +0.9
2,084,569 - yeeF b2014 0.62 -4.9
2,084,606 - yeeF b2014 0.65 +1.8
2,084,606 - yeeF b2014 0.65 -0.8
2,084,651 + yeeF b2014 0.68 -1.2
2,084,651 + yeeF b2014 0.68 -1.8
2,084,741 + yeeF b2014 0.75 -3.9
2,084,790 + yeeF b2014 0.78 +0.4
2,084,790 + yeeF b2014 0.78 -0.8
2,084,803 - yeeF b2014 0.79 -2.9
2,084,945 - yeeF b2014 0.90 -3.3
2,084,945 - yeeF b2014 0.90 +2.3
2,085,023 + -1.3
2,085,023 + +0.8
2,085,092 + yoeI b4678 0.25 -1.5
2,085,736 + yeeY b2015 0.41 -0.4
2,085,786 + yeeY b2015 0.47 -1.8
2,085,813 + yeeY b2015 0.49 -5.6
2,085,852 - yeeY b2015 0.54 +0.0
2,085,860 + yeeY b2015 0.55 -5.4
2,085,860 + yeeY b2015 0.55 -0.5
2,085,866 + yeeY b2015 0.55 +0.1
2,086,064 - yeeY b2015 0.76 +0.3
2,086,173 + yeeY b2015 0.88 -0.6
2,086,181 - yeeY b2015 0.89 -0.8
2,086,181 - yeeY b2015 0.89 -3.0
2,086,264 - -1.3
2,086,298 - +1.5
2,086,338 + +1.6
2,086,338 + -4.9
2,086,351 + +0.4
2,086,424 + yeeZ b2016 0.12 -0.9
2,086,458 - yeeZ b2016 0.16 -1.0
2,086,458 - yeeZ b2016 0.16 +1.6
2,086,500 + yeeZ b2016 0.21 +1.0
2,086,556 - yeeZ b2016 0.28 -0.0
2,086,579 + yeeZ b2016 0.30 +0.5
2,086,660 + yeeZ b2016 0.40 -0.1
2,086,660 + yeeZ b2016 0.40 +0.1
2,086,669 + yeeZ b2016 0.41 +3.2
2,086,672 - yeeZ b2016 0.42 +1.9
2,086,672 - yeeZ b2016 0.42 +1.9
2,086,871 - yeeZ b2016 0.66 -1.0
2,086,947 - yeeZ b2016 0.75 +0.6
2,087,058 + yeeZ b2016 0.88 +0.3
2,087,122 + +0.1
2,087,178 - +1.2
2,087,178 - -1.1
2,087,187 - +0.3
2,087,197 - -3.5

Or see this region's nucleotide sequence