Experiment: R1_N4_T1_phage0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt narZ and narU are separated by 81 nucleotides narU and yddJ are separated by 323 nucleotides yddJ and yddK are separated by 38 nucleotides
b1468: narZ - nitrate reductase 2 (NRZ), alpha subunit (NCBI), at 1,536,874 to 1,540,614
narZ
b1469: narU - nitrate/nitrite transporter (NCBI), at 1,540,696 to 1,542,084
narU
b1470: yddJ - hypothetical protein (NCBI), at 1,542,408 to 1,542,743
yddJ
b1471: yddK - hypothetical protein (RefSeq), at 1,542,782 to 1,543,771
yddK
Position (kb)
1540
1541
1542
1543 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1539.813 kb on + strand, within narZ at 1539.875 kb on + strand, within narZ at 1539.875 kb on + strand, within narZ at 1539.884 kb on - strand, within narZ at 1540.463 kb on - strand at 1540.622 kb on + strand at 1540.622 kb on + strand at 1540.720 kb on + strand at 1540.790 kb on + strand at 1540.922 kb on + strand, within narU at 1540.922 kb on + strand, within narU at 1541.002 kb on - strand, within narU at 1541.375 kb on - strand, within narU at 1541.375 kb on - strand, within narU at 1541.535 kb on + strand, within narU at 1541.599 kb on + strand, within narU at 1541.784 kb on + strand, within narU at 1541.795 kb on + strand, within narU at 1542.086 kb on - strand at 1542.094 kb on + strand at 1542.207 kb on - strand at 1542.207 kb on - strand at 1542.314 kb on + strand at 1542.318 kb on + strand at 1542.381 kb on + strand at 1542.439 kb on + strand at 1542.441 kb on + strand at 1542.441 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R1_N4_T1_phage0 remove 1,539,813 + narZ b1468 0.79 +0.3 1,539,875 + narZ b1468 0.80 -0.0 1,539,875 + narZ b1468 0.80 +0.3 1,539,884 - narZ b1468 0.80 -2.2 1,540,463 - -0.1 1,540,622 + -4.7 1,540,622 + +0.1 1,540,720 + +0.3 1,540,790 + -1.4 1,540,922 + narU b1469 0.16 -0.2 1,540,922 + narU b1469 0.16 +1.2 1,541,002 - narU b1469 0.22 +0.7 1,541,375 - narU b1469 0.49 +1.7 1,541,375 - narU b1469 0.49 +0.9 1,541,535 + narU b1469 0.60 -4.0 1,541,599 + narU b1469 0.65 +1.4 1,541,784 + narU b1469 0.78 +0.6 1,541,795 + narU b1469 0.79 +0.6 1,542,086 - +0.6 1,542,094 + +0.6 1,542,207 - -1.0 1,542,207 - +0.6 1,542,314 + +5.0 1,542,318 + +5.2 1,542,381 + -0.3 1,542,439 + +0.6 1,542,441 + +0.6 1,542,441 + +0.6
Or see this region's nucleotide sequence