Strain Fitness in Escherichia coli BW25113 around b1002

Experiment: R1_N4_T1_phage0

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyccE and agp are separated by 292 nucleotidesagp and yccJ are separated by 37 nucleotidesyccJ and wrbA are separated by 20 nucleotides b1001: yccE - hypothetical protein (NCBI), at 1,063,259 to 1,064,515 yccE b1002: agp - glucose-1-phosphatase/inositol phosphatase (NCBI), at 1,064,808 to 1,066,049 agp b1003: yccJ - hypothetical protein (NCBI), at 1,066,087 to 1,066,314 yccJ b1004: wrbA - TrpR binding protein WrbA (NCBI), at 1,066,335 to 1,066,931 wrbA Position (kb) 1064 1065 1066 1067Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 1064.375 kb on - strand, within yccEat 1064.386 kb on - strand, within yccEat 1064.413 kb on + strandat 1064.413 kb on + strandat 1064.420 kb on + strandat 1064.449 kb on + strandat 1064.610 kb on - strandat 1064.631 kb on - strandat 1064.636 kb on - strandat 1064.649 kb on - strandat 1064.649 kb on - strandat 1064.659 kb on - strandat 1064.669 kb on - strandat 1064.669 kb on - strandat 1064.669 kb on - strandat 1064.669 kb on - strandat 1064.669 kb on - strandat 1064.669 kb on - strandat 1064.669 kb on - strandat 1064.669 kb on - strandat 1064.679 kb on - strandat 1064.728 kb on - strandat 1064.728 kb on - strandat 1064.740 kb on - strandat 1064.783 kb on + strandat 1064.783 kb on - strandat 1064.783 kb on - strandat 1064.794 kb on - strandat 1064.794 kb on - strandat 1064.796 kb on - strandat 1064.808 kb on - strandat 1064.878 kb on - strandat 1064.878 kb on - strandat 1064.896 kb on + strandat 1064.897 kb on + strandat 1064.905 kb on - strandat 1064.967 kb on - strand, within agpat 1064.967 kb on - strand, within agpat 1065.010 kb on + strand, within agpat 1065.018 kb on - strand, within agpat 1065.018 kb on - strand, within agpat 1065.018 kb on - strand, within agpat 1065.028 kb on - strand, within agpat 1065.045 kb on + strand, within agpat 1065.055 kb on - strand, within agpat 1065.055 kb on - strand, within agpat 1065.124 kb on - strand, within agpat 1065.143 kb on - strand, within agpat 1065.144 kb on + strand, within agpat 1065.162 kb on - strand, within agpat 1065.339 kb on + strand, within agpat 1065.578 kb on + strand, within agpat 1065.620 kb on - strand, within agpat 1065.634 kb on - strand, within agpat 1065.634 kb on - strand, within agpat 1065.655 kb on - strand, within agpat 1065.692 kb on + strand, within agpat 1065.699 kb on + strand, within agpat 1065.750 kb on - strand, within agpat 1065.750 kb on - strand, within agpat 1065.803 kb on + strand, within agpat 1065.846 kb on + strand, within agpat 1065.899 kb on - strand, within agpat 1065.901 kb on + strand, within agpat 1065.901 kb on + strand, within agpat 1065.936 kb on - strandat 1065.983 kb on + strandat 1066.079 kb on + strandat 1066.415 kb on + strand, within wrbAat 1066.460 kb on - strand, within wrbAat 1066.550 kb on + strand, within wrbAat 1066.550 kb on + strand, within wrbAat 1066.562 kb on - strand, within wrbAat 1066.562 kb on - strand, within wrbAat 1066.563 kb on - strand, within wrbAat 1066.630 kb on + strand, within wrbAat 1066.651 kb on + strand, within wrbAat 1066.821 kb on - strand, within wrbAat 1066.849 kb on - strand, within wrbAat 1066.911 kb on - strandat 1066.947 kb on + strandat 1066.951 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R1_N4_T1_phage0
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1,064,375 - yccE b1001 0.89 +1.0
1,064,386 - yccE b1001 0.90 +1.0
1,064,413 + -1.4
1,064,413 + +1.0
1,064,420 + +1.0
1,064,449 + -1.0
1,064,610 - +2.0
1,064,631 - -3.0
1,064,636 - -2.4
1,064,649 - -0.6
1,064,649 - -2.7
1,064,659 - +0.1
1,064,669 - +0.0
1,064,669 - -4.8
1,064,669 - +2.8
1,064,669 - -1.0
1,064,669 - -0.0
1,064,669 - -1.1
1,064,669 - -2.3
1,064,669 - -0.6
1,064,679 - -3.8
1,064,728 - +1.4
1,064,728 - +2.0
1,064,740 - +2.0
1,064,783 + +1.0
1,064,783 - +0.4
1,064,783 - -1.6
1,064,794 - -0.4
1,064,794 - -0.2
1,064,796 - -0.2
1,064,808 - +1.0
1,064,878 - +1.2
1,064,878 - +1.6
1,064,896 + -2.0
1,064,897 + -0.6
1,064,905 - +0.1
1,064,967 - agp b1002 0.13 +1.8
1,064,967 - agp b1002 0.13 +2.3
1,065,010 + agp b1002 0.16 -4.5
1,065,018 - agp b1002 0.17 +1.4
1,065,018 - agp b1002 0.17 -4.2
1,065,018 - agp b1002 0.17 -0.4
1,065,028 - agp b1002 0.18 -4.1
1,065,045 + agp b1002 0.19 +0.6
1,065,055 - agp b1002 0.20 -0.5
1,065,055 - agp b1002 0.20 +0.2
1,065,124 - agp b1002 0.25 -0.9
1,065,143 - agp b1002 0.27 +2.5
1,065,144 + agp b1002 0.27 +1.2
1,065,162 - agp b1002 0.29 +1.3
1,065,339 + agp b1002 0.43 -1.4
1,065,578 + agp b1002 0.62 +0.2
1,065,620 - agp b1002 0.65 +1.4
1,065,634 - agp b1002 0.67 +4.0
1,065,634 - agp b1002 0.67 -1.6
1,065,655 - agp b1002 0.68 +3.2
1,065,692 + agp b1002 0.71 +1.4
1,065,699 + agp b1002 0.72 +1.1
1,065,750 - agp b1002 0.76 +1.2
1,065,750 - agp b1002 0.76 -4.0
1,065,803 + agp b1002 0.80 +0.1
1,065,846 + agp b1002 0.84 +2.3
1,065,899 - agp b1002 0.88 -0.2
1,065,901 + agp b1002 0.88 +0.7
1,065,901 + agp b1002 0.88 +1.5
1,065,936 - +0.4
1,065,983 + +0.0
1,066,079 + -2.9
1,066,415 + wrbA b1004 0.13 -0.9
1,066,460 - wrbA b1004 0.21 -0.1
1,066,550 + wrbA b1004 0.36 +0.6
1,066,550 + wrbA b1004 0.36 -3.6
1,066,562 - wrbA b1004 0.38 -5.3
1,066,562 - wrbA b1004 0.38 -0.0
1,066,563 - wrbA b1004 0.38 +0.6
1,066,630 + wrbA b1004 0.49 +1.0
1,066,651 + wrbA b1004 0.53 +1.0
1,066,821 - wrbA b1004 0.81 -0.4
1,066,849 - wrbA b1004 0.86 -3.3
1,066,911 - -4.2
1,066,947 + +0.2
1,066,951 - -1.4

Or see this region's nucleotide sequence