Strain Fitness in Escherichia coli BW25113 around b0879

Experiment: R1_N4_T1_phage0

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb0878 and macB overlap by 4 nucleotidesmacB and cspD are separated by 72 nucleotidescspD and clpS are separated by 322 nucleotidesclpS and clpA are separated by 30 nucleotides b0878: b0878 - putative membrane protein (VIMSS), at 918,458 to 919,573 b0878 b0879: macB - fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component (NCBI), at 919,570 to 921,516 macB b0880: cspD - cold shock protein homolog (NCBI), at 921,589 to 921,813 cspD b0881: clpS - ATP-dependent Clp protease adaptor protein ClpS (NCBI), at 922,136 to 922,456 clpS b0882: clpA - ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI), at 922,487 to 924,763 clpA Position (kb) 919 920 921 922Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 918.620 kb on + strand, within b0878at 918.789 kb on - strand, within b0878at 918.849 kb on - strandat 918.849 kb on - strand, within b0878at 918.985 kb on + strand, within b0878at 919.227 kb on - strand, within b0878at 919.262 kb on + strand, within b0878at 919.262 kb on + strand, within b0878at 919.298 kb on - strand, within b0878at 919.298 kb on - strand, within b0878at 919.343 kb on + strand, within b0878at 919.557 kb on - strandat 919.636 kb on + strandat 919.765 kb on + strand, within macBat 919.979 kb on + strand, within macBat 919.979 kb on + strand, within macBat 919.994 kb on - strand, within macBat 919.994 kb on - strand, within macBat 920.012 kb on + strandat 920.023 kb on + strand, within macBat 920.068 kb on - strand, within macBat 920.199 kb on - strand, within macBat 920.267 kb on + strand, within macBat 920.415 kb on - strand, within macBat 920.443 kb on + strand, within macBat 920.458 kb on - strand, within macBat 920.459 kb on + strand, within macBat 920.459 kb on + strand, within macBat 920.712 kb on - strand, within macBat 920.767 kb on - strand, within macBat 920.849 kb on + strand, within macBat 920.979 kb on + strand, within macBat 921.004 kb on + strand, within macBat 921.164 kb on + strand, within macBat 921.169 kb on + strand, within macBat 921.254 kb on - strand, within macBat 921.254 kb on - strand, within macBat 921.271 kb on + strand, within macBat 921.298 kb on + strand, within macBat 921.298 kb on + strand, within macBat 921.790 kb on + strand, within cspDat 921.790 kb on + strand, within cspDat 921.956 kb on - strandat 921.956 kb on - strandat 922.042 kb on + strandat 922.042 kb on + strandat 922.055 kb on + strandat 922.069 kb on + strandat 922.091 kb on - strandat 922.103 kb on + strandat 922.124 kb on + strandat 922.246 kb on - strand, within clpSat 922.417 kb on + strand, within clpSat 922.435 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R1_N4_T1_phage0
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918,620 + b0878 b0878 0.15 +1.3
918,789 - b0878 b0878 0.30 -3.7
918,849 - +1.0
918,849 - b0878 b0878 0.35 -3.2
918,985 + b0878 b0878 0.47 -1.6
919,227 - b0878 b0878 0.69 -4.1
919,262 + b0878 b0878 0.72 -0.1
919,262 + b0878 b0878 0.72 +0.9
919,298 - b0878 b0878 0.75 -0.2
919,298 - b0878 b0878 0.75 +0.6
919,343 + b0878 b0878 0.79 -4.7
919,557 - -3.5
919,636 + +0.5
919,765 + macB b0879 0.10 +0.3
919,979 + macB b0879 0.21 -0.4
919,979 + macB b0879 0.21 +0.7
919,994 - macB b0879 0.22 -0.6
919,994 - macB b0879 0.22 +1.3
920,012 + -2.3
920,023 + macB b0879 0.23 -0.4
920,068 - macB b0879 0.26 -0.0
920,199 - macB b0879 0.32 +0.6
920,267 + macB b0879 0.36 -0.9
920,415 - macB b0879 0.43 +3.3
920,443 + macB b0879 0.45 -4.3
920,458 - macB b0879 0.46 +0.8
920,459 + macB b0879 0.46 +0.6
920,459 + macB b0879 0.46 +1.3
920,712 - macB b0879 0.59 +1.4
920,767 - macB b0879 0.61 -0.6
920,849 + macB b0879 0.66 -0.7
920,979 + macB b0879 0.72 +0.6
921,004 + macB b0879 0.74 -1.1
921,164 + macB b0879 0.82 +0.7
921,169 + macB b0879 0.82 +0.3
921,254 - macB b0879 0.86 +2.2
921,254 - macB b0879 0.86 +1.0
921,271 + macB b0879 0.87 -0.6
921,298 + macB b0879 0.89 -4.4
921,298 + macB b0879 0.89 +1.7
921,790 + cspD b0880 0.89 -0.6
921,790 + cspD b0880 0.89 -1.1
921,956 - -0.4
921,956 - -0.7
922,042 + +1.0
922,042 + -1.3
922,055 + -0.3
922,069 + -0.0
922,091 - -1.6
922,103 + -0.0
922,124 + -0.5
922,246 - clpS b0881 0.34 +0.5
922,417 + clpS b0881 0.88 +0.8
922,435 - -1.8

Or see this region's nucleotide sequence