Strain Fitness in Escherichia coli BW25113 around b0163

Experiment: R1_N4_T1_phage0

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyaeG and yaeH are separated by 88 nucleotidesyaeH and yaeI are separated by 161 nucleotides b0162: yaeG - orf, hypothetical protein (VIMSS), at 182,463 to 183,620 yaeG b0163: yaeH - hypothetical protein (NCBI), at 183,709 to 184,095 yaeH b0164: yaeI - orf, hypothetical protein (VIMSS), at 184,257 to 185,069 yaeI Position (kb) 183 184 185Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 182.741 kb on + strand, within yaeGat 182.891 kb on + strand, within yaeGat 182.891 kb on + strand, within yaeGat 182.896 kb on + strand, within yaeGat 183.060 kb on - strand, within yaeGat 183.113 kb on - strand, within yaeGat 183.113 kb on - strand, within yaeGat 183.181 kb on + strand, within yaeGat 183.229 kb on + strand, within yaeGat 183.237 kb on - strand, within yaeGat 183.275 kb on + strand, within yaeGat 183.337 kb on - strand, within yaeGat 183.510 kb on + strandat 183.518 kb on - strandat 183.518 kb on - strandat 183.543 kb on - strandat 183.584 kb on + strandat 183.584 kb on + strandat 183.643 kb on + strandat 183.663 kb on + strandat 183.663 kb on + strandat 183.672 kb on + strandat 183.904 kb on - strand, within yaeHat 183.924 kb on - strand, within yaeHat 183.929 kb on + strand, within yaeHat 183.937 kb on - strand, within yaeHat 183.943 kb on + strand, within yaeHat 184.035 kb on + strand, within yaeHat 184.100 kb on - strandat 184.130 kb on + strandat 184.193 kb on - strandat 184.283 kb on - strandat 184.283 kb on - strandat 184.312 kb on + strandat 184.351 kb on - strand, within yaeIat 184.439 kb on + strand, within yaeIat 184.455 kb on + strand, within yaeIat 184.579 kb on + strand, within yaeIat 184.622 kb on - strand, within yaeIat 184.636 kb on - strand, within yaeIat 184.636 kb on - strand, within yaeIat 184.701 kb on - strand, within yaeIat 184.755 kb on + strand, within yaeIat 184.757 kb on - strand, within yaeIat 184.899 kb on - strand, within yaeIat 184.899 kb on - strand, within yaeIat 184.928 kb on + strand, within yaeIat 184.928 kb on + strand, within yaeIat 184.993 kb on - strandat 184.993 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R1_N4_T1_phage0
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182,741 + yaeG b0162 0.24 -1.1
182,891 + yaeG b0162 0.37 +0.1
182,891 + yaeG b0162 0.37 -1.5
182,896 + yaeG b0162 0.37 +0.2
183,060 - yaeG b0162 0.52 +1.0
183,113 - yaeG b0162 0.56 +0.0
183,113 - yaeG b0162 0.56 -3.0
183,181 + yaeG b0162 0.62 -1.9
183,229 + yaeG b0162 0.66 +0.7
183,237 - yaeG b0162 0.67 -0.9
183,275 + yaeG b0162 0.70 -0.9
183,337 - yaeG b0162 0.75 +1.0
183,510 + -1.7
183,518 - +0.1
183,518 - -0.3
183,543 - +1.2
183,584 + +1.0
183,584 + -0.8
183,643 + -0.4
183,663 + -0.2
183,663 + -1.3
183,672 + +1.6
183,904 - yaeH b0163 0.50 +0.2
183,924 - yaeH b0163 0.56 -0.7
183,929 + yaeH b0163 0.57 -2.5
183,937 - yaeH b0163 0.59 +0.5
183,943 + yaeH b0163 0.60 -3.2
184,035 + yaeH b0163 0.84 -0.9
184,100 - -4.5
184,130 + -0.7
184,193 - -0.1
184,283 - +0.3
184,283 - +0.9
184,312 + +0.1
184,351 - yaeI b0164 0.12 -1.8
184,439 + yaeI b0164 0.22 +0.9
184,455 + yaeI b0164 0.24 +0.1
184,579 + yaeI b0164 0.40 +4.3
184,622 - yaeI b0164 0.45 +0.1
184,636 - yaeI b0164 0.47 +0.3
184,636 - yaeI b0164 0.47 +0.7
184,701 - yaeI b0164 0.55 +1.0
184,755 + yaeI b0164 0.61 -0.4
184,757 - yaeI b0164 0.62 -0.0
184,899 - yaeI b0164 0.79 +1.3
184,899 - yaeI b0164 0.79 +1.7
184,928 + yaeI b0164 0.83 -0.7
184,928 + yaeI b0164 0.83 -0.3
184,993 - +0.9
184,993 - +0.9

Or see this region's nucleotide sequence