Experiment: R1_N4_T1_phage0
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hofC and hofB overlap by 11 nucleotides hofB and ppdD are separated by 9 nucleotides ppdD and nadC are separated by 202 nucleotides
b0106: hofC - assembly protein in type IV pilin biogenesis, transmembrane protein (NCBI), at 114,522 to 115,724
hofC
b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099
hofB
b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549
ppdD
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
Position (kb)
115
116
117
118 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 114.838 kb on + strand, within hofC at 114.838 kb on + strand, within hofC at 114.844 kb on + strand, within hofC at 114.928 kb on - strand, within hofC at 114.930 kb on - strand at 114.930 kb on - strand, within hofC at 114.998 kb on + strand, within hofC at 114.998 kb on + strand, within hofC at 115.010 kb on - strand, within hofC at 115.021 kb on + strand, within hofC at 115.021 kb on + strand, within hofC at 115.077 kb on - strand, within hofC at 115.152 kb on - strand, within hofC at 115.152 kb on - strand, within hofC at 115.201 kb on - strand, within hofC at 115.203 kb on - strand, within hofC at 115.203 kb on - strand, within hofC at 115.273 kb on + strand, within hofC at 115.278 kb on + strand, within hofC at 115.278 kb on + strand, within hofC at 115.330 kb on + strand, within hofC at 115.367 kb on + strand, within hofC at 115.367 kb on + strand, within hofC at 115.379 kb on + strand, within hofC at 115.379 kb on + strand, within hofC at 115.409 kb on + strand, within hofC at 115.474 kb on - strand, within hofC at 115.507 kb on + strand, within hofC at 115.507 kb on + strand, within hofC at 115.515 kb on - strand, within hofC at 115.624 kb on + strand at 115.651 kb on + strand at 115.699 kb on - strand at 115.737 kb on + strand at 115.856 kb on - strand, within hofB at 115.856 kb on - strand, within hofB at 115.910 kb on + strand, within hofB at 115.911 kb on - strand, within hofB at 115.911 kb on - strand, within hofB at 115.941 kb on + strand, within hofB at 115.980 kb on + strand, within hofB at 116.058 kb on + strand, within hofB at 116.149 kb on - strand, within hofB at 116.335 kb on + strand, within hofB at 116.399 kb on + strand, within hofB at 116.430 kb on - strand, within hofB at 116.430 kb on - strand, within hofB at 116.471 kb on - strand, within hofB at 116.760 kb on - strand, within hofB at 116.777 kb on + strand, within hofB at 116.777 kb on + strand, within hofB at 116.872 kb on + strand, within hofB at 116.882 kb on + strand, within hofB at 116.957 kb on - strand, within hofB at 116.964 kb on - strand at 117.046 kb on + strand at 117.056 kb on + strand at 117.069 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.081 kb on - strand at 117.081 kb on - strand at 117.084 kb on + strand at 117.087 kb on + strand at 117.227 kb on - strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.293 kb on - strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.385 kb on + strand, within ppdD at 117.387 kb on + strand, within ppdD at 117.508 kb on - strand at 117.508 kb on - strand at 117.511 kb on - strand at 117.511 kb on - strand at 117.525 kb on - strand at 117.525 kb on - strand at 117.603 kb on + strand at 117.603 kb on + strand at 117.839 kb on + strand at 117.856 kb on - strand, within nadC at 117.856 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.881 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 117.922 kb on - strand, within nadC
Per-strain Table
Position Strand Gene LocusTag Fraction R1_N4_T1_phage0 remove 114,838 + hofC b0106 0.26 +0.9 114,838 + hofC b0106 0.26 -3.7 114,844 + hofC b0106 0.27 -0.2 114,928 - hofC b0106 0.34 +0.3 114,930 - +3.2 114,930 - hofC b0106 0.34 +1.0 114,998 + hofC b0106 0.40 -4.2 114,998 + hofC b0106 0.40 -3.3 115,010 - hofC b0106 0.41 -4.7 115,021 + hofC b0106 0.41 +0.6 115,021 + hofC b0106 0.41 +1.4 115,077 - hofC b0106 0.46 -0.7 115,152 - hofC b0106 0.52 +1.4 115,152 - hofC b0106 0.52 -0.4 115,201 - hofC b0106 0.56 -0.8 115,203 - hofC b0106 0.57 +0.0 115,203 - hofC b0106 0.57 -2.1 115,273 + hofC b0106 0.62 -0.2 115,278 + hofC b0106 0.63 -3.6 115,278 + hofC b0106 0.63 +2.3 115,330 + hofC b0106 0.67 +0.3 115,367 + hofC b0106 0.70 +2.6 115,367 + hofC b0106 0.70 -0.8 115,379 + hofC b0106 0.71 -3.8 115,379 + hofC b0106 0.71 -3.9 115,409 + hofC b0106 0.74 +0.4 115,474 - hofC b0106 0.79 -0.2 115,507 + hofC b0106 0.82 +0.6 115,507 + hofC b0106 0.82 -2.8 115,515 - hofC b0106 0.83 -0.2 115,624 + -0.2 115,651 + -4.7 115,699 - +0.2 115,737 + -0.7 115,856 - hofB b0107 0.10 -0.4 115,856 - hofB b0107 0.10 +1.4 115,910 + hofB b0107 0.14 -1.2 115,911 - hofB b0107 0.14 -1.6 115,911 - hofB b0107 0.14 -0.3 115,941 + hofB b0107 0.16 -0.4 115,980 + hofB b0107 0.19 +0.6 116,058 + hofB b0107 0.25 -0.7 116,149 - hofB b0107 0.31 -2.4 116,335 + hofB b0107 0.45 +0.6 116,399 + hofB b0107 0.49 -4.6 116,430 - hofB b0107 0.52 +0.1 116,430 - hofB b0107 0.52 -0.3 116,471 - hofB b0107 0.55 -0.5 116,760 - hofB b0107 0.75 -1.1 116,777 + hofB b0107 0.77 -1.8 116,777 + hofB b0107 0.77 -3.7 116,872 + hofB b0107 0.84 -0.2 116,882 + hofB b0107 0.84 +0.8 116,957 - hofB b0107 0.90 -0.8 116,964 - -1.6 117,046 + -1.7 117,056 + +0.3 117,069 + +0.2 117,075 + -0.2 117,075 + -3.1 117,075 + -0.7 117,081 - +1.1 117,081 - -1.2 117,084 + -1.4 117,087 + +0.5 117,227 - ppdD b0108 0.27 +0.1 117,281 + ppdD b0108 0.39 +1.2 117,281 + ppdD b0108 0.39 +0.2 117,293 - ppdD b0108 0.42 +0.2 117,302 + ppdD b0108 0.44 +1.1 117,302 + ppdD b0108 0.44 -3.2 117,385 + ppdD b0108 0.63 -3.1 117,387 + ppdD b0108 0.63 -4.5 117,508 - -2.8 117,508 - +0.3 117,511 - -0.5 117,511 - -1.6 117,525 - +2.6 117,525 - -1.4 117,603 + +0.3 117,603 + +1.9 117,839 + +0.5 117,856 - nadC b0109 0.12 -3.9 117,856 - nadC b0109 0.12 -1.1 117,879 - nadC b0109 0.14 -0.9 117,879 - nadC b0109 0.14 -4.4 117,881 - nadC b0109 0.14 +0.8 117,922 - nadC b0109 0.19 +1.2 117,922 - nadC b0109 0.19 -4.5
Or see this region's nucleotide sequence