Strain Fitness in Escherichia coli BW25113 around b4596

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciT and yciZ are separated by 89 nucleotidesyciZ and gmr are separated by 147 nucleotides b1284: yciT - predicted DNA-binding transcriptional regulator (NCBI), at 1,341,621 to 1,342,370 yciT b4596: yciZ - hypothetical protein (RefSeq), at 1,342,460 to 1,342,633 yciZ b1285: gmr - modulator of Rnase II stability (NCBI), at 1,342,781 to 1,344,766 gmr Position (kb) 1342 1343Strain fitness (log2 ratio) -1 0 1 2 3at 1341.465 kb on - strandat 1341.473 kb on - strandat 1341.473 kb on - strandat 1341.483 kb on - strandat 1341.494 kb on - strandat 1341.495 kb on - strandat 1341.495 kb on - strandat 1341.543 kb on - strandat 1341.585 kb on - strandat 1341.585 kb on - strandat 1341.609 kb on - strandat 1341.632 kb on - strandat 1341.645 kb on - strandat 1341.654 kb on + strandat 1341.654 kb on + strandat 1341.656 kb on + strandat 1341.719 kb on + strand, within yciTat 1341.719 kb on + strand, within yciTat 1341.725 kb on - strand, within yciTat 1341.768 kb on + strand, within yciTat 1341.823 kb on - strand, within yciTat 1342.104 kb on + strand, within yciTat 1342.104 kb on + strand, within yciTat 1342.216 kb on - strand, within yciTat 1342.306 kb on - strandat 1342.313 kb on + strandat 1342.313 kb on + strandat 1342.329 kb on + strandat 1342.478 kb on + strand, within yciZat 1342.478 kb on + strand, within yciZat 1342.708 kb on - strandat 1342.809 kb on - strandat 1343.191 kb on - strand, within gmrat 1343.224 kb on + strand, within gmrat 1343.224 kb on + strand, within gmrat 1343.235 kb on + strand, within gmrat 1343.621 kb on + strand, within gmrat 1343.621 kb on + strand, within gmr

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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1,341,465 - -0.0
1,341,473 - -0.0
1,341,473 - -0.0
1,341,483 - -0.3
1,341,494 - +0.3
1,341,495 - +0.1
1,341,495 - +0.2
1,341,543 - +0.4
1,341,585 - +0.2
1,341,585 - +0.9
1,341,609 - -0.2
1,341,632 - +0.3
1,341,645 - +0.3
1,341,654 + -0.6
1,341,654 + -1.7
1,341,656 + -0.4
1,341,719 + yciT b1284 0.13 +0.1
1,341,719 + yciT b1284 0.13 -0.4
1,341,725 - yciT b1284 0.14 +0.0
1,341,768 + yciT b1284 0.20 -0.1
1,341,823 - yciT b1284 0.27 -0.2
1,342,104 + yciT b1284 0.64 -0.3
1,342,104 + yciT b1284 0.64 +0.0
1,342,216 - yciT b1284 0.79 +0.1
1,342,306 - +0.1
1,342,313 + +0.2
1,342,313 + +0.2
1,342,329 + -0.1
1,342,478 + yciZ b4596 0.10 +0.6
1,342,478 + yciZ b4596 0.10 +2.8
1,342,708 - -0.5
1,342,809 - +0.1
1,343,191 - gmr b1285 0.21 -0.2
1,343,224 + gmr b1285 0.22 +0.4
1,343,224 + gmr b1285 0.22 +0.2
1,343,235 + gmr b1285 0.23 -0.7
1,343,621 + gmr b1285 0.42 +0.3
1,343,621 + gmr b1285 0.42 -0.2

Or see this region's nucleotide sequence