Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt afuB and insB-2 are separated by 16 nucleotides insB-2 and insA-2 overlap by 82 nucleotides insA-2 and insX are separated by 238 nucleotides insX and yagB are separated by 64 nucleotides yagB and yagA are separated by 93 nucleotides
b0263: afuB - CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq), at 278,038 to 278,385
afuB
b0264: insB-2 - CP4-6 prophage; IS1 transposase InsAB' (NCBI), at 278,402 to 278,905
insB-2
b0265: insA-2 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 278,824 to 279,099
insA-2
b4505: insX - predicted IS protein (RefSeq), at 279,338 to 279,586
insX
b0266: yagB - orf, hypothetical protein (VIMSS), at 279,651 to 279,959
yagB
b0267: yagA - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 280,053 to 281,207
yagA
Position (kb) 279
280 Strain fitness (log2 ratio) -1
0
1 at 278.350 kb on + strand, within afuB at 279.472 kb on - strand, within insX at 279.476 kb on - strand, within insX at 279.573 kb on + strand at 279.581 kb on - strand at 279.581 kb on - strand at 279.584 kb on + strand at 279.584 kb on + strand at 279.624 kb on - strand at 279.624 kb on - strand at 279.714 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.721 kb on + strand, within yagB at 279.721 kb on + strand, within yagB at 279.727 kb on - strand, within yagB at 279.729 kb on - strand, within yagB at 279.729 kb on - strand, within yagB at 279.747 kb on - strand, within yagB at 279.757 kb on - strand, within yagB at 279.824 kb on - strand, within yagB at 279.829 kb on - strand, within yagB at 279.857 kb on - strand, within yagB at 279.882 kb on + strand, within yagB at 279.882 kb on + strand, within yagB at 279.890 kb on - strand, within yagB at 279.890 kb on - strand, within yagB at 279.909 kb on + strand, within yagB at 279.936 kb on + strand at 280.030 kb on + strand at 280.060 kb on + strand at 280.106 kb on + strand at 280.106 kb on + strand at 280.122 kb on - strand at 280.122 kb on - strand at 280.127 kb on + strand at 280.172 kb on - strand, within yagA at 280.182 kb on - strand, within yagA at 280.184 kb on + strand, within yagA at 280.184 kb on + strand, within yagA at 280.288 kb on + strand, within yagA at 280.288 kb on + strand, within yagA at 280.362 kb on - strand, within yagA at 280.362 kb on - strand, within yagA at 280.370 kb on - strand, within yagA at 280.390 kb on - strand, within yagA at 280.395 kb on + strand, within yagA at 280.395 kb on - strand, within yagA at 280.395 kb on - strand, within yagA at 280.398 kb on - strand, within yagA at 280.581 kb on + strand, within yagA
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 278,350 + afuB b0263 0.90 +0.3 279,472 - insX b4505 0.54 +0.7 279,476 - insX b4505 0.55 +0.3 279,573 + -0.2 279,581 - +0.6 279,581 - +0.8 279,584 + -0.1 279,584 + -0.1 279,624 - -0.2 279,624 - +0.1 279,714 + yagB b0266 0.20 +0.2 279,719 + yagB b0266 0.22 +0.2 279,719 + yagB b0266 0.22 +0.2 279,719 + yagB b0266 0.22 -0.3 279,721 + yagB b0266 0.23 -0.0 279,721 + yagB b0266 0.23 +0.4 279,727 - yagB b0266 0.25 +0.5 279,729 - yagB b0266 0.25 -0.2 279,729 - yagB b0266 0.25 +0.1 279,747 - yagB b0266 0.31 +0.4 279,757 - yagB b0266 0.34 +0.1 279,824 - yagB b0266 0.56 -0.0 279,829 - yagB b0266 0.58 -0.4 279,857 - yagB b0266 0.67 -0.1 279,882 + yagB b0266 0.75 -0.4 279,882 + yagB b0266 0.75 +0.1 279,890 - yagB b0266 0.77 +0.1 279,890 - yagB b0266 0.77 +0.9 279,909 + yagB b0266 0.83 +0.3 279,936 + +0.1 280,030 + -0.0 280,060 + +0.1 280,106 + +0.1 280,106 + +0.3 280,122 - +0.3 280,122 - +0.2 280,127 + -0.0 280,172 - yagA b0267 0.10 -0.3 280,182 - yagA b0267 0.11 -0.3 280,184 + yagA b0267 0.11 +0.6 280,184 + yagA b0267 0.11 +0.5 280,288 + yagA b0267 0.20 +0.3 280,288 + yagA b0267 0.20 +0.2 280,362 - yagA b0267 0.27 +0.6 280,362 - yagA b0267 0.27 +0.2 280,370 - yagA b0267 0.27 +0.3 280,390 - yagA b0267 0.29 +0.0 280,395 + yagA b0267 0.30 -0.0 280,395 - yagA b0267 0.30 -0.4 280,395 - yagA b0267 0.30 +0.9 280,398 - yagA b0267 0.30 -0.1 280,581 + yagA b0267 0.46 +0.1
Or see this region's nucleotide sequence