Strain Fitness in Escherichia coli BW25113 around b3651

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspoT and trmH are separated by 6 nucleotidestrmH and recG are separated by 5 nucleotides b3650: spoT - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI), at 3,820,423 to 3,822,531 spoT b3651: trmH - tRNA (Guanosine-2'-O-)-methyltransferase (NCBI), at 3,822,538 to 3,823,227 trmH b3652: recG - ATP-dependent DNA helicase (NCBI), at 3,823,233 to 3,825,314 recG Position (kb) 3822 3823 3824Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3821.855 kb on + strand, within spoTat 3821.855 kb on + strand, within spoTat 3821.879 kb on + strand, within spoTat 3821.920 kb on - strand, within spoTat 3821.939 kb on + strand, within spoTat 3821.957 kb on - strand, within spoTat 3821.957 kb on - strand, within spoTat 3821.972 kb on - strand, within spoTat 3822.086 kb on + strand, within spoTat 3822.104 kb on - strand, within spoTat 3822.116 kb on - strand, within spoTat 3822.174 kb on - strand, within spoTat 3822.214 kb on - strand, within spoTat 3822.220 kb on + strand, within spoTat 3822.220 kb on + strand, within spoTat 3822.222 kb on + strand, within spoTat 3822.244 kb on + strand, within spoTat 3822.263 kb on - strand, within spoTat 3822.263 kb on - strand, within spoTat 3822.397 kb on - strandat 3822.397 kb on - strandat 3822.401 kb on - strandat 3822.409 kb on - strandat 3822.409 kb on - strandat 3822.417 kb on + strandat 3822.455 kb on + strandat 3822.455 kb on + strandat 3822.493 kb on + strandat 3822.493 kb on + strandat 3822.592 kb on + strandat 3822.615 kb on + strand, within trmHat 3822.615 kb on + strand, within trmHat 3822.615 kb on + strand, within trmHat 3822.618 kb on - strand, within trmHat 3822.635 kb on - strand, within trmHat 3822.733 kb on + strand, within trmHat 3822.746 kb on + strand, within trmHat 3822.746 kb on + strand, within trmHat 3822.749 kb on - strand, within trmHat 3822.761 kb on - strand, within trmHat 3822.778 kb on - strand, within trmHat 3822.796 kb on - strand, within trmHat 3822.799 kb on + strand, within trmHat 3822.799 kb on + strand, within trmHat 3822.807 kb on - strand, within trmHat 3822.922 kb on - strand, within trmHat 3822.930 kb on - strand, within trmHat 3822.930 kb on - strand, within trmHat 3822.945 kb on - strand, within trmHat 3823.040 kb on - strand, within trmHat 3823.089 kb on - strand, within trmHat 3823.117 kb on - strand, within trmHat 3823.179 kb on + strandat 3823.179 kb on + strandat 3823.189 kb on + strandat 3823.189 kb on + strandat 3823.215 kb on - strandat 3823.215 kb on - strandat 3823.231 kb on - strandat 3823.265 kb on + strandat 3823.267 kb on + strandat 3823.285 kb on + strandat 3823.341 kb on + strandat 3823.341 kb on + strandat 3823.379 kb on + strandat 3823.379 kb on + strandat 3823.387 kb on - strandat 3823.387 kb on - strandat 3823.393 kb on + strandat 3823.439 kb on + strandat 3823.482 kb on - strand, within recGat 3823.562 kb on - strand, within recGat 3823.574 kb on + strand, within recGat 3823.582 kb on - strand, within recGat 3823.629 kb on - strand, within recGat 3823.641 kb on + strand, within recGat 3823.649 kb on + strand, within recGat 3823.662 kb on - strand, within recGat 3823.690 kb on - strand, within recGat 3823.690 kb on - strand, within recGat 3823.751 kb on + strand, within recGat 3823.751 kb on + strand, within recGat 3823.776 kb on - strand, within recGat 3823.793 kb on + strand, within recGat 3823.805 kb on + strand, within recGat 3823.823 kb on + strand, within recGat 3823.831 kb on - strand, within recGat 3823.881 kb on - strand, within recGat 3823.883 kb on + strand, within recGat 3823.925 kb on - strand, within recGat 3823.969 kb on + strand, within recGat 3824.052 kb on - strand, within recGat 3824.052 kb on - strand, within recGat 3824.061 kb on + strand, within recGat 3824.085 kb on + strand, within recGat 3824.088 kb on - strand, within recGat 3824.112 kb on + strand, within recGat 3824.142 kb on - strand, within recGat 3824.163 kb on - strand, within recGat 3824.193 kb on + strand, within recG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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3,821,855 + spoT b3650 0.68 -0.4
3,821,855 + spoT b3650 0.68 +0.0
3,821,879 + spoT b3650 0.69 -0.5
3,821,920 - spoT b3650 0.71 -0.7
3,821,939 + spoT b3650 0.72 -0.1
3,821,957 - spoT b3650 0.73 -0.4
3,821,957 - spoT b3650 0.73 -0.2
3,821,972 - spoT b3650 0.73 -2.5
3,822,086 + spoT b3650 0.79 -0.0
3,822,104 - spoT b3650 0.80 -1.0
3,822,116 - spoT b3650 0.80 -0.6
3,822,174 - spoT b3650 0.83 -1.8
3,822,214 - spoT b3650 0.85 -1.8
3,822,220 + spoT b3650 0.85 -1.6
3,822,220 + spoT b3650 0.85 -0.4
3,822,222 + spoT b3650 0.85 -1.3
3,822,244 + spoT b3650 0.86 -0.4
3,822,263 - spoT b3650 0.87 -1.3
3,822,263 - spoT b3650 0.87 -0.1
3,822,397 - -2.0
3,822,397 - -0.1
3,822,401 - -1.2
3,822,409 - +0.1
3,822,409 - -0.7
3,822,417 + -2.2
3,822,455 + -2.1
3,822,455 + -0.9
3,822,493 + -0.2
3,822,493 + +0.6
3,822,592 + -0.1
3,822,615 + trmH b3651 0.11 -0.5
3,822,615 + trmH b3651 0.11 +0.9
3,822,615 + trmH b3651 0.11 +0.3
3,822,618 - trmH b3651 0.12 -2.4
3,822,635 - trmH b3651 0.14 -0.4
3,822,733 + trmH b3651 0.28 +0.3
3,822,746 + trmH b3651 0.30 +0.0
3,822,746 + trmH b3651 0.30 -0.0
3,822,749 - trmH b3651 0.31 -1.9
3,822,761 - trmH b3651 0.32 -1.0
3,822,778 - trmH b3651 0.35 -3.0
3,822,796 - trmH b3651 0.37 -3.3
3,822,799 + trmH b3651 0.38 +0.2
3,822,799 + trmH b3651 0.38 +0.6
3,822,807 - trmH b3651 0.39 -2.9
3,822,922 - trmH b3651 0.56 -4.7
3,822,930 - trmH b3651 0.57 -2.5
3,822,930 - trmH b3651 0.57 -1.5
3,822,945 - trmH b3651 0.59 -2.9
3,823,040 - trmH b3651 0.73 -0.9
3,823,089 - trmH b3651 0.80 -4.9
3,823,117 - trmH b3651 0.84 -3.8
3,823,179 + +0.6
3,823,179 + +0.4
3,823,189 + -2.4
3,823,189 + -1.8
3,823,215 - -3.6
3,823,215 - -3.8
3,823,231 - +0.0
3,823,265 + +0.1
3,823,267 + -0.4
3,823,285 + -2.8
3,823,341 + -0.1
3,823,341 + +0.2
3,823,379 + -4.1
3,823,379 + -1.8
3,823,387 - -3.8
3,823,387 - -3.8
3,823,393 + -4.4
3,823,439 + -4.0
3,823,482 - recG b3652 0.12 -0.4
3,823,562 - recG b3652 0.16 -1.4
3,823,574 + recG b3652 0.16 -2.4
3,823,582 - recG b3652 0.17 -1.1
3,823,629 - recG b3652 0.19 -5.2
3,823,641 + recG b3652 0.20 -3.4
3,823,649 + recG b3652 0.20 -2.1
3,823,662 - recG b3652 0.21 -3.2
3,823,690 - recG b3652 0.22 -3.0
3,823,690 - recG b3652 0.22 -1.8
3,823,751 + recG b3652 0.25 -4.5
3,823,751 + recG b3652 0.25 -4.1
3,823,776 - recG b3652 0.26 -4.3
3,823,793 + recG b3652 0.27 -2.8
3,823,805 + recG b3652 0.27 -5.7
3,823,823 + recG b3652 0.28 -3.9
3,823,831 - recG b3652 0.29 -2.1
3,823,881 - recG b3652 0.31 -2.1
3,823,883 + recG b3652 0.31 -4.0
3,823,925 - recG b3652 0.33 -4.0
3,823,969 + recG b3652 0.35 -3.2
3,824,052 - recG b3652 0.39 -3.3
3,824,052 - recG b3652 0.39 -3.2
3,824,061 + recG b3652 0.40 -4.1
3,824,085 + recG b3652 0.41 -3.5
3,824,088 - recG b3652 0.41 -2.9
3,824,112 + recG b3652 0.42 -3.0
3,824,142 - recG b3652 0.44 -2.7
3,824,163 - recG b3652 0.45 -3.3
3,824,193 + recG b3652 0.46 -2.7

Or see this region's nucleotide sequence