Experiment: LB + carbenicillin regrowth after 48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybO and yghW are separated by 188 nucleotides yghW and yghX are separated by 118 nucleotides
b2997: hybO - hydrogenase 2, small subunit (NCBI), at 3,143,165 to 3,144,283
hybO
b2998: yghW - hypothetical protein (NCBI), at 3,144,472 to 3,144,759
yghW
b4658: yghX - no description, at 3,144,878 to 3,145,713
yghX
Position (kb)
3144
3145 Strain fitness (log2 ratio)
-1
0
1 at 3143.475 kb on + strand, within hybO at 3143.499 kb on - strand, within hybO at 3143.515 kb on - strand, within hybO at 3143.521 kb on - strand, within hybO at 3143.656 kb on + strand, within hybO at 3143.677 kb on - strand at 3143.677 kb on - strand, within hybO at 3143.799 kb on - strand, within hybO at 3143.861 kb on - strand, within hybO at 3143.897 kb on + strand, within hybO at 3143.960 kb on - strand, within hybO at 3143.995 kb on + strand, within hybO at 3144.007 kb on + strand, within hybO at 3144.091 kb on + strand, within hybO at 3144.105 kb on + strand, within hybO at 3144.105 kb on + strand, within hybO at 3144.201 kb on + strand at 3144.202 kb on - strand at 3144.250 kb on + strand at 3144.259 kb on + strand at 3144.259 kb on + strand at 3144.267 kb on + strand at 3144.269 kb on + strand at 3144.269 kb on + strand at 3144.395 kb on + strand at 3144.395 kb on + strand at 3144.398 kb on - strand at 3144.437 kb on - strand at 3144.485 kb on + strand at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.553 kb on + strand, within yghW at 3144.560 kb on - strand, within yghW at 3144.561 kb on - strand, within yghW at 3144.564 kb on + strand, within yghW at 3144.564 kb on + strand, within yghW at 3144.659 kb on - strand, within yghW at 3144.701 kb on - strand, within yghW at 3144.845 kb on + strand at 3144.854 kb on - strand at 3144.854 kb on - strand at 3144.911 kb on + strand at 3144.992 kb on + strand, within yghX at 3144.992 kb on + strand, within yghX at 3145.000 kb on - strand, within yghX at 3145.000 kb on - strand, within yghX at 3145.009 kb on + strand, within yghX at 3145.010 kb on - strand, within yghX at 3145.034 kb on + strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.042 kb on - strand, within yghX at 3145.052 kb on - strand, within yghX at 3145.080 kb on + strand, within yghX at 3145.085 kb on + strand, within yghX at 3145.152 kb on + strand, within yghX at 3145.157 kb on + strand, within yghX at 3145.240 kb on + strand, within yghX at 3145.496 kb on - strand, within yghX at 3145.501 kb on - strand, within yghX at 3145.507 kb on + strand, within yghX at 3145.507 kb on + strand, within yghX at 3145.573 kb on + strand, within yghX at 3145.581 kb on - strand, within yghX at 3145.581 kb on - strand, within yghX at 3145.640 kb on + strand at 3145.641 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr remove 3,143,475 + hybO b2997 0.28 -0.1 3,143,499 - hybO b2997 0.30 +0.6 3,143,515 - hybO b2997 0.31 +0.9 3,143,521 - hybO b2997 0.32 +0.6 3,143,656 + hybO b2997 0.44 +0.5 3,143,677 - -0.0 3,143,677 - hybO b2997 0.46 +0.0 3,143,799 - hybO b2997 0.57 +0.3 3,143,861 - hybO b2997 0.62 +0.6 3,143,897 + hybO b2997 0.65 +0.2 3,143,960 - hybO b2997 0.71 +0.9 3,143,995 + hybO b2997 0.74 +0.7 3,144,007 + hybO b2997 0.75 +0.3 3,144,091 + hybO b2997 0.83 +0.2 3,144,105 + hybO b2997 0.84 +0.2 3,144,105 + hybO b2997 0.84 -0.2 3,144,201 + -0.3 3,144,202 - +0.2 3,144,250 + +0.5 3,144,259 + +0.6 3,144,259 + +0.2 3,144,267 + +0.3 3,144,269 + +0.7 3,144,269 + +0.4 3,144,395 + +0.5 3,144,395 + +0.0 3,144,398 - +0.2 3,144,437 - +0.2 3,144,485 + +0.3 3,144,553 + yghW b2998 0.28 +0.1 3,144,553 + yghW b2998 0.28 +1.1 3,144,553 + yghW b2998 0.28 +0.9 3,144,553 + yghW b2998 0.28 +1.0 3,144,553 + yghW b2998 0.28 +1.5 3,144,560 - yghW b2998 0.31 -0.1 3,144,561 - yghW b2998 0.31 +0.8 3,144,564 + yghW b2998 0.32 +0.4 3,144,564 + yghW b2998 0.32 -0.2 3,144,659 - yghW b2998 0.65 -0.0 3,144,701 - yghW b2998 0.80 +0.3 3,144,845 + -0.3 3,144,854 - +1.0 3,144,854 - +0.2 3,144,911 + -0.2 3,144,992 + yghX b4658 0.14 +0.3 3,144,992 + yghX b4658 0.14 +0.7 3,145,000 - yghX b4658 0.15 +0.4 3,145,000 - yghX b4658 0.15 +0.5 3,145,009 + yghX b4658 0.16 -1.7 3,145,010 - yghX b4658 0.16 +0.4 3,145,034 + yghX b4658 0.19 +0.2 3,145,042 - yghX b4658 0.20 -0.2 3,145,042 - yghX b4658 0.20 -0.3 3,145,042 - yghX b4658 0.20 +0.6 3,145,042 - yghX b4658 0.20 +0.2 3,145,052 - yghX b4658 0.21 +0.0 3,145,080 + yghX b4658 0.24 -0.1 3,145,085 + yghX b4658 0.25 +0.0 3,145,152 + yghX b4658 0.33 +0.2 3,145,157 + yghX b4658 0.33 -0.1 3,145,240 + yghX b4658 0.43 +0.5 3,145,496 - yghX b4658 0.74 +0.1 3,145,501 - yghX b4658 0.75 +0.0 3,145,507 + yghX b4658 0.75 -0.0 3,145,507 + yghX b4658 0.75 +0.5 3,145,573 + yghX b4658 0.83 +0.4 3,145,581 - yghX b4658 0.84 +0.3 3,145,581 - yghX b4658 0.84 +0.3 3,145,640 + +0.6 3,145,641 - +0.3
Or see this region's nucleotide sequence