Strain Fitness in Escherichia coli BW25113 around b2996

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybB and hybA overlap by 11 nucleotideshybA and hybO are separated by 2 nucleotides b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186 hybB b2996: hybA - hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI), at 3,142,176 to 3,143,162 hybA b2997: hybO - hydrogenase 2, small subunit (NCBI), at 3,143,165 to 3,144,283 hybO Position (kb) 3142 3143 3144Strain fitness (log2 ratio) -1 0 1at 3141.192 kb on - strand, within hybBat 3141.215 kb on + strand, within hybBat 3141.215 kb on + strand, within hybBat 3141.223 kb on - strand, within hybBat 3141.240 kb on - strand, within hybBat 3141.240 kb on - strand, within hybBat 3141.286 kb on - strand, within hybBat 3141.341 kb on - strand, within hybBat 3141.341 kb on - strand, within hybBat 3141.472 kb on + strand, within hybBat 3141.486 kb on + strand, within hybBat 3141.554 kb on - strand, within hybBat 3141.632 kb on + strand, within hybBat 3141.642 kb on + strand, within hybBat 3141.642 kb on + strand, within hybBat 3141.657 kb on - strand, within hybBat 3141.676 kb on - strand, within hybBat 3141.733 kb on - strand, within hybBat 3141.738 kb on - strand, within hybBat 3141.738 kb on - strand, within hybBat 3141.751 kb on - strand, within hybBat 3141.751 kb on - strand, within hybBat 3141.779 kb on - strand, within hybBat 3141.804 kb on - strand, within hybBat 3141.808 kb on + strand, within hybBat 3141.843 kb on + strand, within hybBat 3141.851 kb on - strand, within hybBat 3141.888 kb on + strand, within hybBat 3141.901 kb on - strand, within hybBat 3141.901 kb on - strand, within hybBat 3141.913 kb on - strand, within hybBat 3141.955 kb on - strand, within hybBat 3141.955 kb on - strand, within hybBat 3142.110 kb on - strandat 3142.110 kb on - strandat 3142.271 kb on - strandat 3142.284 kb on - strand, within hybAat 3142.284 kb on - strand, within hybAat 3142.284 kb on - strand, within hybAat 3142.286 kb on - strand, within hybAat 3142.286 kb on - strand, within hybAat 3142.368 kb on + strand, within hybAat 3142.410 kb on + strand, within hybAat 3142.446 kb on - strand, within hybAat 3142.585 kb on - strand, within hybAat 3142.585 kb on - strand, within hybAat 3142.587 kb on + strand, within hybAat 3142.600 kb on + strand, within hybAat 3142.676 kb on - strand, within hybAat 3142.719 kb on - strand, within hybAat 3142.719 kb on - strand, within hybAat 3142.865 kb on + strand, within hybAat 3142.877 kb on + strand, within hybAat 3142.885 kb on - strand, within hybAat 3142.995 kb on - strand, within hybAat 3142.995 kb on - strand, within hybAat 3143.008 kb on + strand, within hybAat 3143.108 kb on - strandat 3143.108 kb on - strandat 3143.127 kb on + strandat 3143.127 kb on + strandat 3143.223 kb on + strandat 3143.230 kb on + strandat 3143.230 kb on + strandat 3143.277 kb on - strand, within hybOat 3143.279 kb on - strand, within hybOat 3143.410 kb on - strand, within hybOat 3143.410 kb on - strand, within hybOat 3143.456 kb on + strand, within hybOat 3143.471 kb on - strand, within hybOat 3143.475 kb on + strand, within hybOat 3143.499 kb on - strand, within hybOat 3143.515 kb on - strand, within hybOat 3143.521 kb on - strand, within hybOat 3143.656 kb on + strand, within hybOat 3143.677 kb on - strandat 3143.677 kb on - strand, within hybOat 3143.799 kb on - strand, within hybOat 3143.861 kb on - strand, within hybOat 3143.897 kb on + strand, within hybOat 3143.960 kb on - strand, within hybOat 3143.995 kb on + strand, within hybOat 3144.007 kb on + strand, within hybOat 3144.091 kb on + strand, within hybOat 3144.105 kb on + strand, within hybOat 3144.105 kb on + strand, within hybO

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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3,141,192 - hybB b2995 0.16 +0.8
3,141,215 + hybB b2995 0.18 +0.5
3,141,215 + hybB b2995 0.18 +0.6
3,141,223 - hybB b2995 0.18 +0.3
3,141,240 - hybB b2995 0.20 +0.3
3,141,240 - hybB b2995 0.20 +0.0
3,141,286 - hybB b2995 0.24 +0.1
3,141,341 - hybB b2995 0.28 -0.2
3,141,341 - hybB b2995 0.28 +0.3
3,141,472 + hybB b2995 0.39 +0.7
3,141,486 + hybB b2995 0.41 +0.9
3,141,554 - hybB b2995 0.46 +0.8
3,141,632 + hybB b2995 0.53 -0.1
3,141,642 + hybB b2995 0.54 +1.0
3,141,642 + hybB b2995 0.54 +0.1
3,141,657 - hybB b2995 0.55 -0.5
3,141,676 - hybB b2995 0.57 +0.3
3,141,733 - hybB b2995 0.61 +0.1
3,141,738 - hybB b2995 0.62 +0.3
3,141,738 - hybB b2995 0.62 +0.2
3,141,751 - hybB b2995 0.63 +0.1
3,141,751 - hybB b2995 0.63 +0.4
3,141,779 - hybB b2995 0.65 +0.0
3,141,804 - hybB b2995 0.68 +0.4
3,141,808 + hybB b2995 0.68 +0.2
3,141,843 + hybB b2995 0.71 +0.4
3,141,851 - hybB b2995 0.72 -0.0
3,141,888 + hybB b2995 0.75 +0.4
3,141,901 - hybB b2995 0.76 +0.6
3,141,901 - hybB b2995 0.76 +0.2
3,141,913 - hybB b2995 0.77 +0.5
3,141,955 - hybB b2995 0.80 +0.1
3,141,955 - hybB b2995 0.80 +0.3
3,142,110 - +0.4
3,142,110 - +0.3
3,142,271 - +0.3
3,142,284 - hybA b2996 0.11 +0.2
3,142,284 - hybA b2996 0.11 +1.1
3,142,284 - hybA b2996 0.11 +0.2
3,142,286 - hybA b2996 0.11 +0.6
3,142,286 - hybA b2996 0.11 +0.3
3,142,368 + hybA b2996 0.19 +0.4
3,142,410 + hybA b2996 0.24 +0.8
3,142,446 - hybA b2996 0.27 +0.6
3,142,585 - hybA b2996 0.41 +0.4
3,142,585 - hybA b2996 0.41 +1.1
3,142,587 + hybA b2996 0.42 +0.4
3,142,600 + hybA b2996 0.43 +0.1
3,142,676 - hybA b2996 0.51 +0.3
3,142,719 - hybA b2996 0.55 +0.1
3,142,719 - hybA b2996 0.55 +0.2
3,142,865 + hybA b2996 0.70 +0.6
3,142,877 + hybA b2996 0.71 +0.3
3,142,885 - hybA b2996 0.72 +0.3
3,142,995 - hybA b2996 0.83 +0.8
3,142,995 - hybA b2996 0.83 +0.4
3,143,008 + hybA b2996 0.84 +0.8
3,143,108 - +0.1
3,143,108 - +1.2
3,143,127 + +0.5
3,143,127 + +0.3
3,143,223 + +0.7
3,143,230 + +0.0
3,143,230 + -0.1
3,143,277 - hybO b2997 0.10 +1.1
3,143,279 - hybO b2997 0.10 -0.3
3,143,410 - hybO b2997 0.22 +0.3
3,143,410 - hybO b2997 0.22 +0.5
3,143,456 + hybO b2997 0.26 +0.5
3,143,471 - hybO b2997 0.27 +0.6
3,143,475 + hybO b2997 0.28 -0.1
3,143,499 - hybO b2997 0.30 +0.6
3,143,515 - hybO b2997 0.31 +0.9
3,143,521 - hybO b2997 0.32 +0.6
3,143,656 + hybO b2997 0.44 +0.5
3,143,677 - -0.0
3,143,677 - hybO b2997 0.46 +0.0
3,143,799 - hybO b2997 0.57 +0.3
3,143,861 - hybO b2997 0.62 +0.6
3,143,897 + hybO b2997 0.65 +0.2
3,143,960 - hybO b2997 0.71 +0.9
3,143,995 + hybO b2997 0.74 +0.7
3,144,007 + hybO b2997 0.75 +0.3
3,144,091 + hybO b2997 0.83 +0.2
3,144,105 + hybO b2997 0.84 +0.2
3,144,105 + hybO b2997 0.84 -0.2

Or see this region's nucleotide sequence