Strain Fitness in Escherichia coli BW25113 around b2899

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygfZ and yqfA are separated by 195 nucleotidesyqfA and yqfB are separated by 163 nucleotidesyqfB and bglA are separated by 38 nucleotides b2898: ygfZ - putative global regulator (NCBI), at 3,039,335 to 3,040,315 ygfZ b2899: yqfA - predicted oxidoreductase, inner membrane subunit (NCBI), at 3,040,511 to 3,041,170 yqfA b2900: yqfB - hypothetical protein (NCBI), at 3,041,334 to 3,041,645 yqfB b2901: bglA - 6-phospho-beta-glucosidase A (NCBI), at 3,041,684 to 3,043,123 bglA Position (kb) 3040 3041 3042Strain fitness (log2 ratio) -1 0 1 2at 3040.336 kb on - strandat 3040.374 kb on - strandat 3040.405 kb on - strandat 3040.555 kb on - strandat 3040.682 kb on + strand, within yqfAat 3040.731 kb on + strand, within yqfAat 3040.766 kb on + strand, within yqfAat 3040.780 kb on + strand, within yqfAat 3040.811 kb on + strand, within yqfAat 3040.894 kb on + strand, within yqfAat 3040.959 kb on - strand, within yqfAat 3040.959 kb on - strand, within yqfAat 3040.961 kb on - strand, within yqfAat 3040.984 kb on + strand, within yqfAat 3040.984 kb on + strand, within yqfAat 3041.039 kb on + strand, within yqfAat 3041.066 kb on - strand, within yqfAat 3041.186 kb on + strandat 3041.186 kb on + strandat 3041.250 kb on - strandat 3041.449 kb on + strand, within yqfBat 3041.479 kb on - strand, within yqfBat 3041.480 kb on - strand, within yqfBat 3041.529 kb on + strand, within yqfBat 3041.529 kb on + strand, within yqfBat 3041.581 kb on + strand, within yqfBat 3041.607 kb on - strand, within yqfBat 3041.790 kb on + strandat 3041.798 kb on + strandat 3041.798 kb on + strandat 3041.907 kb on - strand, within bglAat 3041.907 kb on - strand, within bglAat 3041.907 kb on - strand, within bglAat 3041.920 kb on + strand, within bglAat 3041.920 kb on + strand, within bglAat 3042.006 kb on - strand, within bglAat 3042.053 kb on + strand, within bglAat 3042.067 kb on + strand, within bglAat 3042.067 kb on + strand, within bglAat 3042.083 kb on - strand, within bglAat 3042.087 kb on - strand, within bglAat 3042.110 kb on - strand, within bglAat 3042.132 kb on + strand, within bglAat 3042.140 kb on - strand, within bglA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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3,040,336 - +0.2
3,040,374 - +0.1
3,040,405 - +0.2
3,040,555 - +0.1
3,040,682 + yqfA b2899 0.26 +0.5
3,040,731 + yqfA b2899 0.33 +0.4
3,040,766 + yqfA b2899 0.39 +0.5
3,040,780 + yqfA b2899 0.41 +0.1
3,040,811 + yqfA b2899 0.45 -0.0
3,040,894 + yqfA b2899 0.58 +1.9
3,040,959 - yqfA b2899 0.68 +0.4
3,040,959 - yqfA b2899 0.68 +0.4
3,040,961 - yqfA b2899 0.68 +0.3
3,040,984 + yqfA b2899 0.72 +0.6
3,040,984 + yqfA b2899 0.72 +0.4
3,041,039 + yqfA b2899 0.80 +0.6
3,041,066 - yqfA b2899 0.84 +0.5
3,041,186 + +0.2
3,041,186 + +0.3
3,041,250 - +0.7
3,041,449 + yqfB b2900 0.37 +0.3
3,041,479 - yqfB b2900 0.46 +0.4
3,041,480 - yqfB b2900 0.47 +0.8
3,041,529 + yqfB b2900 0.62 +0.0
3,041,529 + yqfB b2900 0.62 -0.5
3,041,581 + yqfB b2900 0.79 +0.2
3,041,607 - yqfB b2900 0.88 +0.2
3,041,790 + +0.4
3,041,798 + +0.7
3,041,798 + +0.3
3,041,907 - bglA b2901 0.15 +0.3
3,041,907 - bglA b2901 0.15 -0.1
3,041,907 - bglA b2901 0.15 +0.3
3,041,920 + bglA b2901 0.16 +0.2
3,041,920 + bglA b2901 0.16 +0.8
3,042,006 - bglA b2901 0.22 +0.1
3,042,053 + bglA b2901 0.26 +0.3
3,042,067 + bglA b2901 0.27 +0.1
3,042,067 + bglA b2901 0.27 +0.5
3,042,083 - bglA b2901 0.28 +0.4
3,042,087 - bglA b2901 0.28 +0.5
3,042,110 - bglA b2901 0.30 -0.1
3,042,132 + bglA b2901 0.31 +0.4
3,042,140 - bglA b2901 0.32 +0.4

Or see this region's nucleotide sequence