Strain Fitness in Escherichia coli BW25113 around b2014

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeE and yeeF are separated by 178 nucleotidesyeeF and yoeI overlap by 11 nucleotidesyoeI and yeeY are separated by 214 nucleotides b2013: yeeE - predicted inner membrane protein (NCBI), at 2,082,491 to 2,083,549 yeeE b2014: yeeF - putative amino acid/amine transport protein (VIMSS), at 2,083,728 to 2,085,086 yeeF b4678: yoeI - expressed protein (RefSeq), at 2,085,076 to 2,085,138 yoeI b2015: yeeY - putative transcriptional regulator LYSR-type (VIMSS), at 2,085,353 to 2,086,282 yeeY Position (kb) 2083 2084 2085 2086Strain fitness (log2 ratio) -2 -1 0 1at 2082.775 kb on - strand, within yeeEat 2082.775 kb on - strand, within yeeEat 2082.851 kb on - strand, within yeeEat 2082.881 kb on + strand, within yeeEat 2082.897 kb on - strand, within yeeEat 2082.897 kb on - strand, within yeeEat 2082.915 kb on - strand, within yeeEat 2082.973 kb on + strand, within yeeEat 2082.973 kb on + strand, within yeeEat 2082.981 kb on - strand, within yeeEat 2083.040 kb on - strand, within yeeEat 2083.040 kb on - strand, within yeeEat 2083.126 kb on + strand, within yeeEat 2083.139 kb on + strand, within yeeEat 2083.183 kb on + strand, within yeeEat 2083.186 kb on - strand, within yeeEat 2083.186 kb on - strand, within yeeEat 2083.258 kb on - strand, within yeeEat 2083.310 kb on + strand, within yeeEat 2083.319 kb on - strand, within yeeEat 2083.319 kb on - strand, within yeeEat 2083.462 kb on + strandat 2083.470 kb on + strandat 2083.498 kb on - strandat 2083.498 kb on - strandat 2083.498 kb on - strandat 2083.537 kb on - strandat 2083.693 kb on - strandat 2083.807 kb on + strandat 2083.877 kb on - strand, within yeeFat 2083.887 kb on - strand, within yeeFat 2083.939 kb on + strand, within yeeFat 2083.957 kb on + strand, within yeeFat 2083.996 kb on + strand, within yeeFat 2084.160 kb on + strand, within yeeFat 2084.160 kb on + strand, within yeeFat 2084.200 kb on - strand, within yeeFat 2084.206 kb on - strand, within yeeFat 2084.260 kb on + strand, within yeeFat 2084.276 kb on + strand, within yeeFat 2084.284 kb on + strand, within yeeFat 2084.284 kb on + strand, within yeeFat 2084.421 kb on + strand, within yeeFat 2084.421 kb on + strand, within yeeFat 2084.450 kb on - strand, within yeeFat 2084.462 kb on - strand, within yeeFat 2084.569 kb on - strand, within yeeFat 2084.606 kb on - strand, within yeeFat 2084.606 kb on - strand, within yeeFat 2084.651 kb on + strand, within yeeFat 2084.651 kb on + strand, within yeeFat 2084.741 kb on + strand, within yeeFat 2084.790 kb on + strand, within yeeFat 2084.790 kb on + strand, within yeeFat 2084.803 kb on - strand, within yeeFat 2084.945 kb on - strand, within yeeFat 2084.945 kb on - strand, within yeeFat 2085.023 kb on + strandat 2085.023 kb on + strandat 2085.092 kb on + strand, within yoeIat 2085.736 kb on + strand, within yeeYat 2085.786 kb on + strand, within yeeYat 2085.813 kb on + strand, within yeeYat 2085.852 kb on - strand, within yeeYat 2085.860 kb on + strand, within yeeYat 2085.860 kb on + strand, within yeeYat 2085.866 kb on + strand, within yeeYat 2086.064 kb on - strand, within yeeY

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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2,082,775 - yeeE b2013 0.27 +0.6
2,082,775 - yeeE b2013 0.27 +0.7
2,082,851 - yeeE b2013 0.34 +0.1
2,082,881 + yeeE b2013 0.37 +0.9
2,082,897 - yeeE b2013 0.38 +0.3
2,082,897 - yeeE b2013 0.38 +0.3
2,082,915 - yeeE b2013 0.40 +0.1
2,082,973 + yeeE b2013 0.46 -0.6
2,082,973 + yeeE b2013 0.46 +0.8
2,082,981 - yeeE b2013 0.46 +0.1
2,083,040 - yeeE b2013 0.52 -0.5
2,083,040 - yeeE b2013 0.52 -0.3
2,083,126 + yeeE b2013 0.60 +0.8
2,083,139 + yeeE b2013 0.61 +0.0
2,083,183 + yeeE b2013 0.65 +0.7
2,083,186 - yeeE b2013 0.66 -0.1
2,083,186 - yeeE b2013 0.66 +0.6
2,083,258 - yeeE b2013 0.72 -0.2
2,083,310 + yeeE b2013 0.77 -0.0
2,083,319 - yeeE b2013 0.78 +0.3
2,083,319 - yeeE b2013 0.78 -0.3
2,083,462 + -0.1
2,083,470 + +0.2
2,083,498 - +0.8
2,083,498 - +0.5
2,083,498 - +0.2
2,083,537 - -0.3
2,083,693 - -0.8
2,083,807 + +0.4
2,083,877 - yeeF b2014 0.11 +0.2
2,083,887 - yeeF b2014 0.12 +0.3
2,083,939 + yeeF b2014 0.16 +0.9
2,083,957 + yeeF b2014 0.17 -0.5
2,083,996 + yeeF b2014 0.20 +1.5
2,084,160 + yeeF b2014 0.32 +0.3
2,084,160 + yeeF b2014 0.32 +0.7
2,084,200 - yeeF b2014 0.35 +0.1
2,084,206 - yeeF b2014 0.35 +0.7
2,084,260 + yeeF b2014 0.39 +0.7
2,084,276 + yeeF b2014 0.40 +0.4
2,084,284 + yeeF b2014 0.41 +0.6
2,084,284 + yeeF b2014 0.41 +0.9
2,084,421 + yeeF b2014 0.51 -0.8
2,084,421 + yeeF b2014 0.51 +0.7
2,084,450 - yeeF b2014 0.53 +0.6
2,084,462 - yeeF b2014 0.54 +0.3
2,084,569 - yeeF b2014 0.62 +0.6
2,084,606 - yeeF b2014 0.65 +1.1
2,084,606 - yeeF b2014 0.65 +0.1
2,084,651 + yeeF b2014 0.68 -0.8
2,084,651 + yeeF b2014 0.68 +0.9
2,084,741 + yeeF b2014 0.75 +0.2
2,084,790 + yeeF b2014 0.78 +0.5
2,084,790 + yeeF b2014 0.78 +0.4
2,084,803 - yeeF b2014 0.79 -0.0
2,084,945 - yeeF b2014 0.90 -0.2
2,084,945 - yeeF b2014 0.90 +1.5
2,085,023 + +0.5
2,085,023 + +0.6
2,085,092 + yoeI b4678 0.25 +0.9
2,085,736 + yeeY b2015 0.41 +0.4
2,085,786 + yeeY b2015 0.47 -1.9
2,085,813 + yeeY b2015 0.49 -0.2
2,085,852 - yeeY b2015 0.54 +0.3
2,085,860 + yeeY b2015 0.55 +0.0
2,085,860 + yeeY b2015 0.55 +0.2
2,085,866 + yeeY b2015 0.55 +0.6
2,086,064 - yeeY b2015 0.76 +0.1

Or see this region's nucleotide sequence