Strain Fitness in Escherichia coli BW25113 around b1919

Experiment: LB + carbenicillin regrowth after 48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyecC and yecS overlap by 4 nucleotidesyecS and yedO are separated by 14 nucleotidesyedO and fliY are separated by 104 nucleotidesfliY and fliZ are separated by 87 nucleotides b1917: yecC - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 1,995,086 to 1,995,838 yecC b1918: yecS - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 1,995,835 to 1,996,503 yecS b1919: yedO - putative 1-aminocyclopropane-1-carboxylate deaminase (VIMSS), at 1,996,518 to 1,997,504 yedO b1920: fliY - cystine transporter subunit (NCBI), at 1,997,609 to 1,998,409 fliY b1921: fliZ - orf, hypothetical protein (VIMSS), at 1,998,497 to 1,999,048 fliZ Position (kb) 1996 1997 1998Strain fitness (log2 ratio) -1 0 1at 1995.592 kb on + strand, within yecCat 1995.598 kb on - strand, within yecCat 1995.598 kb on - strand, within yecCat 1995.688 kb on + strand, within yecCat 1995.771 kb on - strandat 1995.777 kb on - strandat 1995.777 kb on - strandat 1995.779 kb on + strandat 1995.779 kb on + strandat 1995.779 kb on + strandat 1995.779 kb on + strandat 1995.880 kb on + strandat 1995.880 kb on + strandat 1995.968 kb on + strand, within yecSat 1995.976 kb on - strand, within yecSat 1996.355 kb on + strand, within yecSat 1996.445 kb on - strandat 1996.445 kb on - strandat 1996.446 kb on - strandat 1996.778 kb on - strand, within yedOat 1997.064 kb on - strand, within yedOat 1997.231 kb on - strand, within yedOat 1997.231 kb on - strand, within yedOat 1997.256 kb on - strand, within yedOat 1997.658 kb on + strandat 1997.811 kb on + strand, within fliYat 1997.811 kb on + strand, within fliYat 1997.931 kb on - strand, within fliYat 1998.266 kb on + strand, within fliYat 1998.375 kb on + strandat 1998.375 kb on + strandat 1998.387 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin regrowth after 48hr
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1,995,592 + yecC b1917 0.67 +0.1
1,995,598 - yecC b1917 0.68 +0.4
1,995,598 - yecC b1917 0.68 +0.2
1,995,688 + yecC b1917 0.80 +0.4
1,995,771 - +0.0
1,995,777 - -0.1
1,995,777 - +0.1
1,995,779 + -0.3
1,995,779 + +0.1
1,995,779 + +0.2
1,995,779 + -0.3
1,995,880 + +0.1
1,995,880 + +0.7
1,995,968 + yecS b1918 0.20 -0.1
1,995,976 - yecS b1918 0.21 +0.4
1,996,355 + yecS b1918 0.78 +0.1
1,996,445 - +0.3
1,996,445 - +0.4
1,996,446 - +0.2
1,996,778 - yedO b1919 0.26 +0.6
1,997,064 - yedO b1919 0.55 +0.3
1,997,231 - yedO b1919 0.72 +1.5
1,997,231 - yedO b1919 0.72 +0.4
1,997,256 - yedO b1919 0.75 +0.3
1,997,658 + +0.4
1,997,811 + fliY b1920 0.25 +0.0
1,997,811 + fliY b1920 0.25 +0.4
1,997,931 - fliY b1920 0.40 +0.0
1,998,266 + fliY b1920 0.82 +0.0
1,998,375 + -1.0
1,998,375 + -0.2
1,998,387 - +0.1

Or see this region's nucleotide sequence